Abstract
Simultaneous induction of vitamin E deficiency exacerbates most of the Se-deficiency disorders in animals (Thompson and Scott 1969, 1970). These two nutrients have sparing effects upon the dietary requirement for both (Bieri 1961; Hamilton and Tappel 1963), and hence are considered to have overlapping, complementary functions in the antioxidative defense mechanisms in the cells of different mammalian organs. The quantitative significance remains yet unclear, however, regarding the protection afforded by glutathione peroxidase (GSH-Px) compared with that afforded by vitamin E (Tappel 1980; Levander 1986). This chapter presents three lines of experimental evidence pointing to the predominant function of GSH-Px in antioxidant defense mechanisms.
To whom all correspondence should be addressed
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bieri JG (1961) The nature of the action of selenium in replacing vitamin E. Am J Clin Nutr 9:89— 96
Bieri JG, Poukka RKH (1970) In vitro hemolysis as related to rat erythrocyte content of atocopherol and polyunsaturated fatty acids. J Nutr 100: 557–564
Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37: 911–917
Hamilton JW, Tappel AL (1963) Lipid antioxidant activity in tissues and proteins of selenium-fed animals J Nutr 79: 493–502
Kim CH, Yasumoto K, Suzuki T, Yoshida M (1988) Failure of glutathione in suppressing tertbutyl hydroperoxide-induced hemolysis in vitro of selenium-deficient rat erythrocytes. Nutr Res 8: 767–775
Kim CH, Yasumoto K, Suzuki T, Yoshida M (1989) tert-Butyl hydroperoxide-induced hemolysis of a-tocopherol-decreased erythrocytes from selenium-deficient and selenium-adequate rats. J Nutr Sci Vitaminol 34: 481–490
Levander OA (1986) Selenium. In: Mertz W (ed) Trace elements in human and animal nutrition, 2nd edn. Academic Press, London New York, pp 209–279
Merril CR, Dunau ML, Goldman D (1981) A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal Biochem 110: 201–207
Rice-Evans C, Baysal E, Hochstein P (1985) t-Butyl hydroperoxide-induced perturbations of human erythrocytes as a model for oxidant stress. Biochim Biophys Acta 815: 426–432
Rouser G, Fleischer S, Yamamoto A (1970) Two-dimensional thin-layer chromatographic separation of polar lipids and determination of phospholipids by phosphorus analysis of spots. Lipids 5: 494–496
Seaman C, Wyss S, Piomelli S (1980) The decline in energetic metabolism with aging of the erythrocyte and its relationship to cell death. Am J Hematol 8: 31–42
Seaman C, Wyss S, Piomelli S (1980) The decline in energetic metabolism with aging of the erythrocyte and its relationship to cell death. Am J Hematol 8: 31–42
Tanaka H, Mino M (1986) Membrane-to-membrane transfer of tocopherol in red blood cells. J Nutr Sci Vitaminol 32: 463–474
Tappel AL (1980) Vitamin E and selenium protection from in vivo lipid peroxidation. In: Levander OA, Cheng L (eds) Micronutrient interactions: vitamin, minerals, and hazardous elements. NY Acad Sci, New York, pp 18–31
Thompson JN, Scott ML (1969) Role of selenium in the nutrition of the chick. J Nutr 97:335 —342
Thompson JN, Scott ML (1970) Impaired lipid and vitamin E absorption related to atrophy of the pancreas in selenium-deficient chicks. J Nutr 100: 797–809
Yamamoto Y, Niki E, Eguchi J, Kamiya Y, Shimasaki H (1985) Oxidation of biological membranes and its inhibition: free radical chain oxidation of erythrocyte ghost membranes by oxygen. Biochim Biophys Acta 819: 29–36
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Yasumoto, K., Kim, C.H., Suzuki, T. (1989). Role of Glutathione Peroxidase and α-Tocopherol in Protecting Rat Erythrocytes Against tert-Butyl Hydroperoxide-Induced Hemolysis in Vitro. In: Wendel, A. (eds) Selenium in Biology and Medicine. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74421-1_13
Download citation
DOI: https://doi.org/10.1007/978-3-642-74421-1_13
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-74423-5
Online ISBN: 978-3-642-74421-1
eBook Packages: Springer Book Archive