Advertisement

Molecular Composition, Fluidity of Membranes and Functional Properties of Human Liver Mitochondria and Microsomes

  • Gh. Benga

Abstract

Liver is a particularly useful tissue for the characterization of subcellular organelles since the purified fractions prepared from liver are derived mainly from a single cell type, the hepatocyte. This is not only because hepatocytes constitute 78% of the volume of liver (Blouin et al. 1977) but also because they are the major cell type broken by homogenization of the tissue (Dallner et al. 1966).

Keywords

Fatty Acid Composition Human Liver Brown Adipose Tissue Liver Mitochondrion Spin Label 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Benga Gh (1974) Fracţionarea subcelulară a ţesutului hepatic uman. I. Izolarea fracţiunii mitocondriale. St Cere Biochim 17:123–135Google Scholar
  2. Benga Gh (1975) Fracţionarea subcelulară a ficatului uman. II. Caracterizarea mitocondriilor in condiţii patologice. St Cerc Biochim 18:83–91Google Scholar
  3. Benga Gh, Borza V (1975) Differences in reactivity of cytochrome oxidase from human liver mitochondria with horse and human cytochrome c. Arch Biochem Biophys 169:354–357CrossRefGoogle Scholar
  4. Benga Gh (1983) Spin labelling. In: Wrigglesworth J (ed) Biochemical research techniques. John Willey & Sons, New York London, pp 79–117Google Scholar
  5. Benga Gh (ed) (1985) Structure and properties of cell membranes, vol 1-3. CRC, Boca RatonGoogle Scholar
  6. Benga Gh, Ferdinand W (1977) Increased content of hydrophobic amino acid residues in lipid-rich mitochondrial membranes: a comparison of rat and human liver mitochondria. Int J Biochem 8:17–20CrossRefGoogle Scholar
  7. Benga Gh, Holmes RP (1984) Interactions between components in biological membranes and their implications for membrane function. Prog Biophys Mol Biol 43:195–257PubMedCrossRefGoogle Scholar
  8. Benga Gh, Mureşan L (1974) Human liver mitochondria. III. ATPase activity as an index of mitochondrial damage. Biochem Med 10:131–145PubMedCrossRefGoogle Scholar
  9. Benga Gh, Tărmure C, Hodâmău A (1971) The determination of proteins in lipid-rich mitochondrial suspensions. Enzyme 12:574–577PubMedGoogle Scholar
  10. Benga Gh, Mureşan L, Hodârnău A, Dancea S (1972) Conditions for isolation and study of enzymic properties of human liver mitochondria. Biochem Med 6:508–521PubMedCrossRefGoogle Scholar
  11. Benga Gh, Hodârnău A, Böhm B, Borza V, Tilinca R, Dancea S, Petrescu I (1978) Human liver mitochondria: relation of a particular lipid composition to the mobility of spin-labelled lipids. Eur J Biochem 84:625–633PubMedCrossRefGoogle Scholar
  12. Benga Gh, Hodârnău A, Tilinca R, Porutiu D, Dancea S, Pop V, Wrigglesworth J (1979). Fractionation of human liver mitochondria: enzymic and morphological characterization of the inner and outer membranes as compared to rat liver mitochondria. J Cell Sci 35:417–429PubMedGoogle Scholar
  13. Benga Gh, Petrescu I, Tärmure C, Pop V (1980) Species related functional properties of mitochondria; comparison between rat and human liver mitochondria. Rev Roum Biol 25:147–154Google Scholar
  14. Benga Gh, Pop VI, Ionescu M, Hodârnău A, Tilinca R, Frangopol PT (1983) Comparison-of human and rat liver microsomes by spin label and biochemical analyses. Biochim Biophys Acta 750:194–199PubMedGoogle Scholar
  15. Blouin A, Bolender RP, Weibel ER (1977) Distribution of organelles and membranes between hepatocytes and non-hepatocytes in the rat liver parenchyma. J Cell Biol 72:441–445PubMedCrossRefGoogle Scholar
  16. Christiansen EN, Drahota Z, Duszynski J, Wojtczak L (1973) Transport of adenine nucleotides in mitochondria from the brown adipose tissue. Eur J Biochem 34:506–512PubMedCrossRefGoogle Scholar
  17. Dallner G, Siekevitz P, Palade GE (1966) Biogenesis of endoplasmic reticulum membranes. J Cell Biol 30:97–117PubMedCrossRefGoogle Scholar
  18. Dickerson RE (1972) The structure and history of an ancient protein. Sci Am 226:58–72PubMedCrossRefGoogle Scholar
  19. Engelman DM, Steitz TA, Goldman A (1986) Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Chem 15:321–353CrossRefGoogle Scholar
  20. Grav HJ, Pedersen JI, Christiansen EN (1970) Conditions in vitro which affect respiratory control and capacity for respiration-linked phosphorylation in brown adipose tissue. Eur J Biochem 12:11–23PubMedCrossRefGoogle Scholar
  21. Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219:1–14PubMedGoogle Scholar
  22. Hayashida T, Portman OW (1960) Swelling of liver mitochondria from rats fed diets deficient in essential fatty acids. Proc Soc Exp Biol Med 103:656–659PubMedGoogle Scholar
  23. Hwang DH, Chanmugam P, Anding R (1982) Effects of dietary 9-trans, 12-trans linoleate on arachidonic acid metabolism in rat platelets. Lipids 17:307–313PubMedCrossRefGoogle Scholar
  24. Kleemann W, McConnell MM (1974) Lateral phase separations in Escherichia coli membranes. Biochim Biophys Acta 345:220–230PubMedCrossRefGoogle Scholar
  25. Linden CD, Wright KL, McConnell HM, Fox CF (1973) Lateral phase separations in membrane lipids and the mechanism of sugar transport in E. coli. Proc Nat Acad Sci USA 70:2271–2275PubMedCrossRefGoogle Scholar
  26. McConnell HM, McFarland BG (1970) Physics and chemistry of spin labels. Q Rev Biophys 3:91–136PubMedCrossRefGoogle Scholar
  27. Mountford CE, Wright LC (1988) Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem Sci 13:172–177PubMedCrossRefGoogle Scholar
  28. Nicholls DG (1974) Hamster brown-adipose-tissue mitochondria. The control of respiration and the proton electrochemical potential gradient by possible physiological effectors of the proton conductance of the inner membrane. Eur J Biochem 49:573PubMedCrossRefGoogle Scholar
  29. Richardson T, Tappel AL, Gruger EH (1961) Essential fatty acids in mitochondria. Arch Biochem Biophys 94:1–6PubMedCrossRefGoogle Scholar
  30. Richardson T, Tappel AL, Smith LM, Houle CR (1962) Polyunsaturated fatty acids in mitochondria. J Lipid Res 3:344–350Google Scholar
  31. Rieder R, Bosshard HR (1978a) Cytochrome bc 1 and cytochrome oxidase can bind to the same surface domain of the cytochrome c molecule. FEBS Lett 92:223–226PubMedCrossRefGoogle Scholar
  32. Rieder R, Bosshard HR (1978b) The cytochrome c oxidase binding site on cytochrome c. Differential chemical modification of lysine residues in free and oxidase-bound cytochrome c. J Biol Chem 253:6045–6053PubMedGoogle Scholar
  33. Rouser E, Nelson GJ, Fleischer S, Simon G (1968) Lipid composition of animal cell membranes, organelles and organs. In: Chapman D (ed) Biological membranes. Physical fact and function, vol 1. Academic Press, New York London, pp 5–69Google Scholar
  34. Schmidt E, Schmidt FW (1970) Enzyme activities in human liver. Enzymol Biol Clin 11:67–129Google Scholar
  35. Segrest JP, Feldmann RJ (1974) Membrane proteins: amino acid sequence and membrane penetration. J Mol Biol 87:853–858PubMedCrossRefGoogle Scholar
  36. Shimshick EJ, McConnell HM (1973a) Lateral phase separations in phospholipid membranes. Biochemistry 12:2351–2360PubMedCrossRefGoogle Scholar
  37. Shimshick EJ, McConnell HM (1973b) Lateral phase separations in binary mixtures of cholesterol and phospholipids. Biochem Biophys Res Commun 53:446PubMedCrossRefGoogle Scholar
  38. Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175:720–731PubMedCrossRefGoogle Scholar
  39. Smith L, Davies HC, Nava ME (1976) Evidence for binding sites on cytochrome c for oxidases and reductases from studies of different cytochromes c of known structure. Biochemistry 15:5827–5831CrossRefGoogle Scholar
  40. Stancliff RC, Williams MA, Utsumi K, Packer L (1969) Essential fatty acid deficiency and mitochondrial function. Arch Biochem Biophys 131:629–642PubMedCrossRefGoogle Scholar
  41. Toader C, Acalovschi I, Toader I, Manta I, Hodárnáu A, Benga Gh (1976) Factors influencing the establishment of the normal values of the respiratory activities of human liver mitochondria. Enzyme 21:232–242PubMedGoogle Scholar
  42. Van Den Bosch H (1974) Phosphoglyceride metabolism. Annu Rev Biochem 43:243–277PubMedCrossRefGoogle Scholar
  43. Vanderkooi G, Capaldi RA (1972) A comparative study of the amino acid composition of membrane proteins and other proteins. Ann NY Acad Sci 195:135–138PubMedCrossRefGoogle Scholar
  44. Von Heijne G (1981) Membrane proteins. The amino acid composition of membrane penetrating segments. Eur J Biochem 120:275–278CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Gh. Benga
    • 1
  1. 1.Department of Cell Biology, Faculty of MedicineMedical and Pharmaceutical Institute Cluj-Napoca, and Institute of Hygiene and Public HealthCluj-NapocaRumania

Personalised recommendations