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Molecular Basis of Membrane-Associated Diseases pp 285–302Cite as

Molecular Composition, Fluidity of Membranes and Functional Properties of Human Liver Mitochondria and Microsomes

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Abstract

Liver is a particularly useful tissue for the characterization of subcellular organelles since the purified fractions prepared from liver are derived mainly from a single cell type, the hepatocyte. This is not only because hepatocytes constitute 78% of the volume of liver (Blouin et al. 1977) but also because they are the major cell type broken by homogenization of the tissue (Dallner et al. 1966).

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References

  • Benga Gh (1974) Fracţionarea subcelulară a ţesutului hepatic uman. I. Izolarea fracţiunii mitocondriale. St Cere Biochim 17:123–135

    CAS  Google Scholar 

  • Benga Gh (1975) Fracţionarea subcelulară a ficatului uman. II. Caracterizarea mitocondriilor in condiţii patologice. St Cerc Biochim 18:83–91

    Google Scholar 

  • Benga Gh, Borza V (1975) Differences in reactivity of cytochrome oxidase from human liver mitochondria with horse and human cytochrome c. Arch Biochem Biophys 169:354–357

    Article  CAS  Google Scholar 

  • Benga Gh (1983) Spin labelling. In: Wrigglesworth J (ed) Biochemical research techniques. John Willey & Sons, New York London, pp 79–117

    Google Scholar 

  • Benga Gh (ed) (1985) Structure and properties of cell membranes, vol 1-3. CRC, Boca Raton

    Google Scholar 

  • Benga Gh, Ferdinand W (1977) Increased content of hydrophobic amino acid residues in lipid-rich mitochondrial membranes: a comparison of rat and human liver mitochondria. Int J Biochem 8:17–20

    Article  CAS  Google Scholar 

  • Benga Gh, Holmes RP (1984) Interactions between components in biological membranes and their implications for membrane function. Prog Biophys Mol Biol 43:195–257

    Article  PubMed  CAS  Google Scholar 

  • Benga Gh, Mureşan L (1974) Human liver mitochondria. III. ATPase activity as an index of mitochondrial damage. Biochem Med 10:131–145

    Article  PubMed  CAS  Google Scholar 

  • Benga Gh, Tărmure C, Hodâmău A (1971) The determination of proteins in lipid-rich mitochondrial suspensions. Enzyme 12:574–577

    PubMed  CAS  Google Scholar 

  • Benga Gh, Mureşan L, Hodârnău A, Dancea S (1972) Conditions for isolation and study of enzymic properties of human liver mitochondria. Biochem Med 6:508–521

    Article  PubMed  CAS  Google Scholar 

  • Benga Gh, Hodârnău A, Böhm B, Borza V, Tilinca R, Dancea S, Petrescu I (1978) Human liver mitochondria: relation of a particular lipid composition to the mobility of spin-labelled lipids. Eur J Biochem 84:625–633

    Article  PubMed  CAS  Google Scholar 

  • Benga Gh, Hodârnău A, Tilinca R, Porutiu D, Dancea S, Pop V, Wrigglesworth J (1979). Fractionation of human liver mitochondria: enzymic and morphological characterization of the inner and outer membranes as compared to rat liver mitochondria. J Cell Sci 35:417–429

    PubMed  CAS  Google Scholar 

  • Benga Gh, Petrescu I, Tärmure C, Pop V (1980) Species related functional properties of mitochondria; comparison between rat and human liver mitochondria. Rev Roum Biol 25:147–154

    CAS  Google Scholar 

  • Benga Gh, Pop VI, Ionescu M, Hodârnău A, Tilinca R, Frangopol PT (1983) Comparison-of human and rat liver microsomes by spin label and biochemical analyses. Biochim Biophys Acta 750:194–199

    PubMed  CAS  Google Scholar 

  • Blouin A, Bolender RP, Weibel ER (1977) Distribution of organelles and membranes between hepatocytes and non-hepatocytes in the rat liver parenchyma. J Cell Biol 72:441–445

    Article  PubMed  CAS  Google Scholar 

  • Christiansen EN, Drahota Z, Duszynski J, Wojtczak L (1973) Transport of adenine nucleotides in mitochondria from the brown adipose tissue. Eur J Biochem 34:506–512

    Article  PubMed  CAS  Google Scholar 

  • Dallner G, Siekevitz P, Palade GE (1966) Biogenesis of endoplasmic reticulum membranes. J Cell Biol 30:97–117

    Article  PubMed  CAS  Google Scholar 

  • Dickerson RE (1972) The structure and history of an ancient protein. Sci Am 226:58–72

    Article  PubMed  CAS  Google Scholar 

  • Engelman DM, Steitz TA, Goldman A (1986) Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins. Annu Rev Biophys Chem 15:321–353

    Article  CAS  Google Scholar 

  • Grav HJ, Pedersen JI, Christiansen EN (1970) Conditions in vitro which affect respiratory control and capacity for respiration-linked phosphorylation in brown adipose tissue. Eur J Biochem 12:11–23

    Article  PubMed  CAS  Google Scholar 

  • Halliwell B, Gutteridge JMC (1984) Oxygen toxicity, oxygen radicals, transition metals and disease. Biochem J 219:1–14

    PubMed  CAS  Google Scholar 

  • Hayashida T, Portman OW (1960) Swelling of liver mitochondria from rats fed diets deficient in essential fatty acids. Proc Soc Exp Biol Med 103:656–659

    PubMed  CAS  Google Scholar 

  • Hwang DH, Chanmugam P, Anding R (1982) Effects of dietary 9-trans, 12-trans linoleate on arachidonic acid metabolism in rat platelets. Lipids 17:307–313

    Article  PubMed  CAS  Google Scholar 

  • Kleemann W, McConnell MM (1974) Lateral phase separations in Escherichia coli membranes. Biochim Biophys Acta 345:220–230

    Article  PubMed  CAS  Google Scholar 

  • Linden CD, Wright KL, McConnell HM, Fox CF (1973) Lateral phase separations in membrane lipids and the mechanism of sugar transport in E. coli. Proc Nat Acad Sci USA 70:2271–2275

    Article  PubMed  CAS  Google Scholar 

  • McConnell HM, McFarland BG (1970) Physics and chemistry of spin labels. Q Rev Biophys 3:91–136

    Article  PubMed  CAS  Google Scholar 

  • Mountford CE, Wright LC (1988) Organization of lipids in the plasma membranes of malignant and stimulated cells: a new model. Trends Biochem Sci 13:172–177

    Article  PubMed  CAS  Google Scholar 

  • Nicholls DG (1974) Hamster brown-adipose-tissue mitochondria. The control of respiration and the proton electrochemical potential gradient by possible physiological effectors of the proton conductance of the inner membrane. Eur J Biochem 49:573

    Article  PubMed  CAS  Google Scholar 

  • Richardson T, Tappel AL, Gruger EH (1961) Essential fatty acids in mitochondria. Arch Biochem Biophys 94:1–6

    Article  PubMed  CAS  Google Scholar 

  • Richardson T, Tappel AL, Smith LM, Houle CR (1962) Polyunsaturated fatty acids in mitochondria. J Lipid Res 3:344–350

    CAS  Google Scholar 

  • Rieder R, Bosshard HR (1978a) Cytochrome bc 1 and cytochrome oxidase can bind to the same surface domain of the cytochrome c molecule. FEBS Lett 92:223–226

    Article  PubMed  CAS  Google Scholar 

  • Rieder R, Bosshard HR (1978b) The cytochrome c oxidase binding site on cytochrome c. Differential chemical modification of lysine residues in free and oxidase-bound cytochrome c. J Biol Chem 253:6045–6053

    PubMed  CAS  Google Scholar 

  • Rouser E, Nelson GJ, Fleischer S, Simon G (1968) Lipid composition of animal cell membranes, organelles and organs. In: Chapman D (ed) Biological membranes. Physical fact and function, vol 1. Academic Press, New York London, pp 5–69

    Google Scholar 

  • Schmidt E, Schmidt FW (1970) Enzyme activities in human liver. Enzymol Biol Clin 11:67–129

    CAS  Google Scholar 

  • Segrest JP, Feldmann RJ (1974) Membrane proteins: amino acid sequence and membrane penetration. J Mol Biol 87:853–858

    Article  PubMed  CAS  Google Scholar 

  • Shimshick EJ, McConnell HM (1973a) Lateral phase separations in phospholipid membranes. Biochemistry 12:2351–2360

    Article  PubMed  CAS  Google Scholar 

  • Shimshick EJ, McConnell HM (1973b) Lateral phase separations in binary mixtures of cholesterol and phospholipids. Biochem Biophys Res Commun 53:446

    Article  PubMed  CAS  Google Scholar 

  • Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175:720–731

    Article  PubMed  CAS  Google Scholar 

  • Smith L, Davies HC, Nava ME (1976) Evidence for binding sites on cytochrome c for oxidases and reductases from studies of different cytochromes c of known structure. Biochemistry 15:5827–5831

    Article  CAS  Google Scholar 

  • Stancliff RC, Williams MA, Utsumi K, Packer L (1969) Essential fatty acid deficiency and mitochondrial function. Arch Biochem Biophys 131:629–642

    Article  PubMed  CAS  Google Scholar 

  • Toader C, Acalovschi I, Toader I, Manta I, Hodárnáu A, Benga Gh (1976) Factors influencing the establishment of the normal values of the respiratory activities of human liver mitochondria. Enzyme 21:232–242

    PubMed  CAS  Google Scholar 

  • Van Den Bosch H (1974) Phosphoglyceride metabolism. Annu Rev Biochem 43:243–277

    Article  PubMed  Google Scholar 

  • Vanderkooi G, Capaldi RA (1972) A comparative study of the amino acid composition of membrane proteins and other proteins. Ann NY Acad Sci 195:135–138

    Article  PubMed  CAS  Google Scholar 

  • Von Heijne G (1981) Membrane proteins. The amino acid composition of membrane penetrating segments. Eur J Biochem 120:275–278

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Cell Biology, Faculty of Medicine, Medical and Pharmaceutical Institute Cluj-Napoca, and Institute of Hygiene and Public Health, 6 Pasteur St, 3400, Cluj-Napoca, Rumania

    Gh. Benga

Authors
  1. Gh. Benga
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Editor information

Editors and Affiliations

  1. Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstraße 28, CH-3012, Bern, Switzerland

    Angelo Azzi

  2. Institute of Physiology, Czechoslovak Academy of Sciences, Videnska 1083, CS-142 20, Praha 4, Czech Republic

    Zdenek Drahota

  3. Institute of Medical Biochemistry and Chemistry, University of Bari, Piazza G. Cesare, I-70124, Bari, Italy

    Sergio Papa

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© 1989 Springer-Verlag Berlin Heidelberg

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Benga, G. (1989). Molecular Composition, Fluidity of Membranes and Functional Properties of Human Liver Mitochondria and Microsomes. In: Azzi, A., Drahota, Z., Papa, S. (eds) Molecular Basis of Membrane-Associated Diseases. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74415-0_24

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  • DOI: https://doi.org/10.1007/978-3-642-74415-0_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74417-4

  • Online ISBN: 978-3-642-74415-0

  • eBook Packages: Springer Book Archive

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