Advertisement

Mechanisms of Sinusoidal Bile Acid Transport

  • B. L. Blitzer
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)

Abstract

During the past decade, the Na+-dependence of hepatic conjugated bile acid uptake has been well established. Initial studies in the isolated perfused rat liver, isolated rat hepatocytes, and hepatocyte monolayer culture first demonstrated Na+-dependence and ouabain inhibition of taurocholate uptake (for a review, see Blitzer and Boyer, 1982).

Keywords

Bile Acid Conjugate Bile Acid Bile Acid Transport Unconjugated Bile Acid Uphill Transport 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anwer MS, O’Maille ERL, Hofman AF, Di Pietro RA, Michelotti E (1985) Influence of side-chain charge on hepatic transport of bile acids and bile acid analogues. Am J Physiol 249: G479–G488PubMedGoogle Scholar
  2. Aronson PS (1983) Mechanisms of active H+ secretion in the proximal tubule. Am J Physiol 245: F647–F659PubMedGoogle Scholar
  3. Blitzer BL, Boyer JL (1982) Cellular mechanisms of bile formation. Gastroenterology 82: 346–357PubMedGoogle Scholar
  4. Blitzer BL, Bueler RL (1985) Kinetic and energetic aspects of the inhibition of taurocholate uptake by Na+-dependent amino acids: studies in rat liver plasma membrane vesicles. Am J Physiol 249: G120–G124PubMedGoogle Scholar
  5. Blitzer BL, Donovan CB (1984) A new method for the rapid isolation of basolateral plasma membrane vesicles from rat liver: characterization, validation, and bile acid transport studies. J Biol Chem 259: 9295–9301PubMedGoogle Scholar
  6. Blitzer BL, Lyons L (1985) Enhancement of Na+-dependent bile acid uptake by albumin: direct demonstration in rat basolateral liver plasma membrane vesicles. Am J Physiol 249: G34–G38PubMedGoogle Scholar
  7. Blitzer BL, Ratoosh SL, Donovan CB (1983) Amino acid inhibition of bile acid uptake by isolated rat hepatocytes: relationship to dissipation of transmembrane Na+ gradient. Am J Physiol 245: G399–G403PubMedGoogle Scholar
  8. Blitzer BL, Ratoosh SL, Donovan CB, Boyer JL (1982) Effects of inhibitors of Na+-coupled ion transport on bile acid uptake by isolated rat hepatocytes. Am J Physiol 243: G48–G53PubMedGoogle Scholar
  9. Blitzer BL, Terzakis C, Scott KA (1986) Hydroxyl/bi1e acid exchange: a new mechanism for the uphill transport of cholate by basolateral liver plasma membrane vesicles. J Biol Chem 261: 12042–12046PubMedGoogle Scholar
  10. Caflisch C, Zimmerli B, Hugentobler G, Meier PJ (1987) pH gradient driven cholate uptake into rat liver plasma membrane vesicles represents nonionic diffusion rather than a carrier mediated process. Gastroenterology 92: 1722Google Scholar
  11. Dember L, Blitzer BL (1984) Conjugation is a requirement for Na+-coupled bile acid transport by rat basolateral liver plasma membrane vesicles. Gastroenterology 86: 1315Google Scholar
  12. Edmondson JW,Miller BA, Lumeng L (1985) Effect of glucagon on hepatic taurocholate uptake: relationship to membrane potential. Am J Physiol 249: G427–G433PubMedGoogle Scholar
  13. Fitz JG, Scharschmidt BF (1987) Regulation of transmembrane electrical potential in rat hepatocytes in situ. Am J Physiol 252: G56–G64PubMedGoogle Scholar
  14. Forker EL, Luxon BA (1981) Albumin helps mediate removal of taurocholate by rat liver. J Clin Invest 67: 1517–1522PubMedCrossRefGoogle Scholar
  15. Hardison WGM, Bellentani S, Heasley V, Shelhamer D (1984) Specificity of an Na+-dependent taurocholate transport site in isolated rat hepatocytes. Am J Physiol 246: G477–G483PubMedGoogle Scholar
  16. Horie T, Mizuma T, Kasai S, Awazu IM (1982) Taurocholate transport by rat liver sinusoidal membrane vesicles. Am J Physiol 254: G465–G470Google Scholar
  17. Inoue M, Kinne R, Tran T, Arias IM (1982) Taurocholate trans- 4 port by rat liver sinusoidal membrane vesicles. Hepatology 2: 572–579PubMedCrossRefGoogle Scholar
  18. Karniski LP, Aronson PS (1987) Anion exchange pathways for CI- transport in rabbit renal microvillus membranes. Am J Physiol 253: F513–F521PubMedGoogle Scholar
  19. Kramer W, Bickel U, Buscher HP, Gerok W, Kurz G (1982) Bile salt-binding polypeptides in plasma membranes of hepatocytes revealed by photoaffinity labelling. Eur J Biochem 129: 13–24PubMedCrossRefGoogle Scholar
  20. Ledford CH, Blitzer BL (1988) Identification of the hepatic Na+-dependent bile acid transporter through substrate protection and sulfhydryl labelling. Gastroenterology 94: A560Google Scholar
  21. Meier PJ, Meier-Abt AS, Barrett C, Boyer JL (1984) Mechanisms of taurocholate transport in canalicular and basolateral rat liver plasma membrane vesicles. J Biol Chem 259: 10614–10622PubMedGoogle Scholar
  22. Moseley RH? Meier PJ, Aronson PS, Boyer JL (1986) Na-H exchange in rat liver basolateral but not canalicular plasma membrane vesicles. Am J Physiol 250:G35–G43Google Scholar
  23. Roda A, Fini A (1984) Effect of nuclear hydroxy substituents on aqueous solubility and acidic strength of bile acids. Hepatology 4: 72S–76SPubMedCrossRefGoogle Scholar
  24. Von Dippe P, Ananthanarayanan M, Drain P, Levy D (1986) Purification and reconstitution of bile acid transport system from hepatocyte sinusoidal plasma membranes. Biochim Biophys Acta 862: 353–360Google Scholar
  25. Von Dippe P, Levy D (1983) Characterization of the bile acid transport system in normal and transformed hepatocytes. J Biol Chem 258: 8896–8901Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • B. L. Blitzer
    • 1
  1. 1.Liver Study UnitUniversity of Cincinnati College of MedicineCincinnatiUSA

Personalised recommendations