Glutamate-sodium Cotransport in the Kidney: An Example for the Plasticity of Transport Systems

  • Rolf K. H. Kinne
Conference paper
Part of the Proceedings in Life Sciences book series (LIFE SCIENCES)


Glutamate is an important metabolic intermediate as well as a neurotransmitter. Therefore, the question of its transport across plasma membranes has generated considerable interest. In the liver, the results concerning the ability to transport glutamate have been controversial. In liver slices (Hems et al., 1968) and in the perfused liver (Ross et al., 1967) the parenchymal cell membrane was found to be relatively impermeable to glutamate, whereas sodium-dependent glutamate transport was observed in rat hepatocytes in primary monolayer culture (Gebhardt and Mecke, 1983) and in a mixed preparation of plasma membrane vesicles from rat liver (Sips et al., 1982). The discrepancy between these different results was resolved recently by Ballatori et al. (1986) who demonstrated that the sodium-L-glutamate cotransport system was only present in the canalicular domains of liver plasma membranes. Since this domain is not exposed in slices or in the intact organ the strong polarity of the hepatocyte with regard to the distribution of transport systems (Kinne, 1987) could be made responsible for the divergence in the experimental results.


Brush Border Membrane Glutamate Uptake Liver Plasma Membrane Primary Monolayer Culture Cotransport System 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Ballatori N, Moseley RH, Boyer JL (1986) Sodium gradient- dependent L-glutamate transport is localized to the canalicular domain of liver plasma membranes. Studies in rat liver sinusoidal and canalicular membrane vesicles. J Biol Chem 261: 6216–6221PubMedGoogle Scholar
  2. Burckhardt G, Kinne R, Stange G, Murer H (1980) The effects of potassium and membrane potential on sodium-dependent glutamic acid uptake. Biochim Biophys Acta 599: 191–201PubMedCrossRefGoogle Scholar
  3. Fukuhara Y, Turner J (1985) Cation dependence of renal outer cortical brush border membrane L-glutamate transport. Am J Physiol 248: F869–F875PubMedGoogle Scholar
  4. Gebhardt R, Mecke D (1983) Glutamate uptake by cultured rat hepatocytes is mediated by hormonally inducible, sodium- dependent transport systems. FEBS Lett 161: 275–278PubMedCrossRefGoogle Scholar
  5. Heinz E, Sommerfeld DL, Kinne RKH (1988) F.1 ectrogeni ci ty of sodium/L-glutam ate cotransport in rabbit renal brush- border membranes: a réévaluation. Biochim Biophys Acta 937: 300–308PubMedCrossRefGoogle Scholar
  6. Hems R, Stubbs M, Krebs HA (1968) Restricted permeability of rat liver for qlutamate and succinate. Biochem J 107: 807–815PubMedGoogle Scholar
  7. Kinne RKH (1987) Modulation of membrane transport in epi- thelia: lessons for the liver. In: Reutter W, Popper H, Arias IM, Heinrich PC, Keppler D, Landmann L (eds) Modulation of liver cell expression, Falk Symposium 43. MTP Press Ltd, Lancaster/Boston/The Hague/Dordrecht, p 95–106Google Scholar
  8. Koepsell H, Korn K, Ferguson D, Menuhr H, Ollig D, Haase W (1984) Reconstitution and partial purification of several Na+ cotransport systems from renal brush-border membranes. Properties of the L-qlutamate transporter in proteolipo- somes. J Biol Chem 259: 6548–6558PubMedGoogle Scholar
  9. Nelson PJ, Dean GE, Aronson PS, Rudnick G (1983) Hydrogen ion cotransport by the renal brush border glutamate transporter. Biochemistry 22: 5459–5463PubMedCrossRefGoogle Scholar
  10. Ross BD, Hems R, Krebs HA (1967) The rate of g 1 uconeogenesi s from various precursors in the perfused rat liver. Biochem J 102: 942–951PubMedGoogle Scholar
  11. Sacktor B (1981) L-Glutamate transport in renal plasma membrane vesicles. Mol Cell Biochem 39: 239–251PubMedCrossRefGoogle Scholar
  12. Sacktor B, Lepor N, Schneider EG (1981) Stimulation of the efflux of L-glutamate from renal brush-border membrane vesicles by extravesicular potassium. Biosci Rep 1: 709–713PubMedCrossRefGoogle Scholar
  13. Sacktor B, Schneider EG (1980) The singular effect of an internal K+ gradient (Kl+ K+) on the Na+ gradient (Na+ Na+)-dependent transport of L-glutamate in renal brush border membrane vesicles. Int J Biochem 12: 229–234PubMedCrossRefGoogle Scholar
  14. Samarzija I, Fromter E (1975) Electrical studies on amino acid transport across brushborder membrane of rat proximal tubule in vivo. Pfliigers Arch 359: R119Google Scholar
  15. Samarzija I, Fromter E (1976) Renal transport of glutamate and aspartate. Evidence for Na-dependent uptake from the peritubular surface into proximal tubular cells. Pflügers Arch 365: R15CrossRefGoogle Scholar
  16. Schneider EG, Hammerman MR, Sacktor B (1980) Sodium gradient- dependent L-glutamate transport in renal brush border membrane vesicles. Evidence for an e1ectroneutral mechanism. J Biol Chem 255: 7650–7656PubMedGoogle Scholar
  17. Sips HJ, De Graaf PA, Van Dam K (1982) Transport of L-aspartate and L-glutamate in plasma-membrane vesicles from rat liver. Eur J Biochem 122: 259–264PubMedCrossRefGoogle Scholar
  18. Turner RJ (1985) Stoichiometry of cotransport systems. Ann NY Acad Sci 456: 10–25PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Rolf K. H. Kinne
    • 1
  1. 1.Max-Planck-Institut für SystemphysiologieDortmund 1Germany

Personalised recommendations