Summary
Comparison of amino acid sequences of Mn-stabilising protein (MSP) of Anacystis nidulans R2 and spinach reveals 5 clusters of conserved residues. Domains essential for the functioning of MSP are likely to be situated in these clusters. The two cysteine residues of MSP are conserved. They are likely to form a disulfide bond which plays an important role in maintaining the tertiary structure of the protein.
The precursor of the Anacystis MSP possesses an N-terminal signal peptide composed of 28 amino acid residues, the structure for translocation across the thylakoid membrane. The following has been found upon expression of the MSP gene in Escherichia coli. (I) The expression gives a polypeptide of 30 kDa, which is indistinguishable in size from the authentic mature MSP although the gene directs the precursor. (II) The 30-kDa polypeptide is located at the outer surface of the cytoplasmic membrane. These findings suggest that the precursor MSP synthesised in E. coli is processed to the mature protein by a signal peptidase of E. coli.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Murata, N. and Miyao, M. (1985) Trends Biochem. Sci. 10, 122–124
Kuwabara, T. and Murata, N. (1982) Plant Cell Physiol. 23, 533–539
Miyao, M. and Murata, N. (1984) FEBS Lett. 170, 350–354
Ono, T. and Inoue, Y. (1983) FEBS Lett. 164, 255–260
Kuwabara, T., Miyao M., Murata, T. and Murata, N. (1985) Biochim. Biophys. Acta 806, 283–289
Tang, X.-S. and Satoh, K. (1985) FEBS Lett. 179, 60–64
Satoh, K., Ohno, T. and Katoh, S. (1985) FEBS Lett. 180, 326–330
Ikeuchi, M., Yuasa, M. and Inoue, Y. (1985) FEBS Lett. 185, 316–322
Ono, T. and Inoue, Y. (1985) Biochim. Biophys. Acta 806, 331–340
Miyao, M., Murata, N., Lavorel, J., Maison-Peteri, B., Boussac, A. and Etienne, A.-L. (1987) Biochim. Biophys. Acta 890, 151–159
Oh-oka, H., Tanaka, S., Wada, K., Kuwabara, T. and Murata, N. (1986) FEBS Lett. 197, 63–66
Watanabe, A., Minami, E., Murase, M., Shinohara, K., Kuwabara, T. and Murata, N. (1987) in: Progress in Photosynthesis Research, Vol. 4 (Biggins, J., ed.) pp. 629–636, Nijhoff, Dordrecht
Tyagi, A., Hermans, J., Steppuhn, J., Jansson, Ch., Vater, F. and Herrmann, R.G. (1987) Mol. Gen. Genet. 207, 288–293
Kuwabara, T., Reddy K.J. and Sherman, L.A. (1987) Proc. Natl Acad. Sci. USA 84, 8230–8234
Kuwabara, T. and Murata, N. (1979) Biochim. Biophys. Acta 581, 228–236
Tanaka, S. and Wada, K. (1987) Photosynthesis Res., in press
Murata, N., Miyao, M. and Kuwabara, T. (1983) in: The Oxygen Evolving System of Photosynthesis (Inoue, Y., Crofts, A.R., Govindjee, Murata, N., Renger, G., Satoh, K., eds.) pp. 213–222, Academic Press, Tokyo
Andersson, B., Larsson, C., Jansson, C., Ljungberg, U. and Åkerlund, H.-E. (1984) Biochim. Biophys. Acts 766, 21–28
Westhoff, P., Jansson, C., Klein-Hitpass, L., Berzborn, R., Larsson, C. and Bartlett, S.G. (1985) Plant Mol. Biol. 4, 137–146
Minami, E., Shinohara, K., Kuwabara, T. and Watanabe, A. (1986) Arch. Biochem. Biophys. 224, 517–527
Karlin-Neumann, G.A. and Tobin, E.M. (1986) EMBO J. 5, 9–13
Smeekens, S., Bauerle, C., Hageman, J., Keegstra, K. and Weisbeek, P. (1986) Cell 46, 365–375
Chia, C.P. and Arntzen, C.J. (1986) J. Cell Biol. 103, 725–731
Chou, P.Y. and Fasman, G.D. (1978) Annu. Rev. Biochem. 47, 251–276
Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) in: Molecular Cloning: A Laboratory Manual, pp. 440–441, Cold Spring Harbor Laboratory, NY
Minsky, A., Summers, R.G. and Knowles, J.R. (1986) Proc. Natl Acad. Sci. USA, 83, 4180–4184
Hattori, T., Ichihara, S. and Nakamura, K. (1987) Eur. J. Biochem. 166, 533–538
Author information
Authors and Affiliations
Editor information
Rights and permissions
Copyright information
© 1989 Narosa Publishing House
About this paper
Cite this paper
Kuwabara, T. (1989). Primary Structure and Expression in Escherichia coli of the Mn-stabilising Protein Involved in Photosystem II Water Oxidation. In: Singhal, G.S., Barber, J., Dilley, R.A., Govindjee, Haselkorn, R., Mohanty, P. (eds) Photosynthesis. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74221-7_6
Download citation
DOI: https://doi.org/10.1007/978-3-642-74221-7_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-74223-1
Online ISBN: 978-3-642-74221-7
eBook Packages: Springer Book Archive