Photosynthesis pp 331-341 | Cite as

Genotypic Response to Assimilate Demand in a Variegated Leaf Mutant of Nerium Oleander

  • C. C. Subbaiah
  • A. Gnanam


A naturally occurring variegated leaf biotype of Nerium oleander L. was tentatively identified to be a plastome coded mutant. The yellow sectors leaves in the mutant had less than 1–2% of the total quantity of chlorophyll, and 5–8% of the carotenoids, normally found in the wild type; and were totally lacking in light-dependent 02 evolution as well as CO2 fixation. However, the green sectors of the variegated leaves in the mutant compensated for the thinning effect imposed by the heterotrophic yellow tissue, since they had high rates of C02 assimilation. We looked at the basis of the superior photosynthetic efficiency of the green sectors of the mutant leaves, which was two times greater than that in the wild type leaves. The chloroplasts from the green sectors showed greater capacity for light utilisation with increased amounts of electron transport complexes (PQ and Cyt t) and ATP synthase per unit chlorophyll in their thylakoid membranes as compared to those in the wild type, thus resembling the chloroplasts from plants adapted to high irradiance. However, at the gross morphological level the variegated leaves mimicked shade leaves with greater surface areas and greater intercellular air space volume than the wild type leaves. We propose that these contrasting strategies at the organelle and organ levels help the green sectors to achieve greater photosynthetic rates and overcome the heterotrophic stress imposed by the non-green tissue. Also, these responses appeared to involve a co-ordinated gene expression in nucleo-cytoplasmic and plastid compartments.


Thylakoid Membrane Wild Type Leave Green Sector Genotypic Response Yellow Sector 
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  1. 1.
    Byott, G. S. (1976) New Phytol. 76, 295–299CrossRefGoogle Scholar
  2. 2.
    Lichtenthaler, H. K. and Wellburn, A. R. (1983) Biochem. Soc Transactions 603, 591–592Google Scholar
  3. 3.
    Younis, H., Winget, G. D. and Racker, E. (1977) J. Biol. Chem. 252, 1814–1816PubMedGoogle Scholar
  4. 4.
    Delepelaire and Chua, N.-H. (1982) in Methods in Chloroplast Molecular Biology (Edelman, M., Hallick, R. B. and Chua, N. H. eds.) pp.000, Elsevier Biomedical Press, AmsterdamGoogle Scholar
  5. 5.
    Bendall, D. S., Davenport, H. E. and Hill, R. (1971) Methods Enzymol. 23, 327–344CrossRefGoogle Scholar
  6. 6.
    Thomas, P. E., Ryan, D. and Levin, W. (1976) Anal. Biochem. 75, 168–176PubMedCrossRefGoogle Scholar
  7. 7.
    Waering, P. F., Khalifa, M. M. and Treharne, K. J. (1968) Nature (Lond.) 220,453–457CrossRefGoogle Scholar
  8. 8.
    Gifford, R. M. and Evans, L. T. (1981) Annu. Rev. Plant Physiol. 32, 485–509CrossRefGoogle Scholar
  9. 9.
    Wilhelm, C. and Wild, A. (1984) J. Plant Physiol. 115, 125–135Google Scholar
  10. 10.
    Chow, W. S. and Anderson, J. M. (1987) Aust. J. Plant Physiol. 14, 9–19CrossRefGoogle Scholar
  11. 11.
    Bjorkman, O., Boardman, N. K., Anderson, J. M., Thorne, S. W., Goodchild, D. J. and Pyliotis, N. A. (1972) Carengie Inst. Year Book 71, 115–135Google Scholar
  12. 12.
    Grahl, H. and Wild, A. (1975) in Environmental and Biological Control of Photosynthesis (Marcelle, R., ed.) pp. 107–113, Dr. W. Junk Publishers, The HagueGoogle Scholar
  13. 13.
    Berzborn, R. J., Müller, D., Roos, P. and Anderson, B. (1981) in Photosynthesis III. Structure and Molecular Organization of the Photosynthetic Apparatus (Akoyunoglou, G., ed.) pp. 107–120, Balban Intern. Science Series, PhiladelphiaGoogle Scholar
  14. 14.
    Leong, T. Y. and Anderson, J. M. (1984) Photosyn. Res. 5, 117–128CrossRefGoogle Scholar
  15. 15.
    Davies, E. C, Chow, W. S., LeFay, J. M. and Jordan, R. R. (1986) J. Exp. Bot. 37, 211–220CrossRefGoogle Scholar
  16. 16.
    Anderson, J. M. (1986) Annu. Rev. Plant Physiol. 37, 93–136CrossRefGoogle Scholar

Copyright information

© Narosa Publishing House 1989

Authors and Affiliations

  • C. C. Subbaiah
    • 1
  • A. Gnanam
    • 1
  1. 1.Department of Plant SciencesMadurai Kamaraj UniversityMaduraiIndia

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