The Albino Perinatal Lethal Mutation: Identification of Affected mRNAs and Mapping of the Locus by Pulsed-Field Gel Electrophoresis

  • Gavin D. Kelsey
  • Siegfried Ruppert
  • Michael Burchart
  • Andreas Schedl
  • Wolfgang Schmid
  • Günther Schütz
Part of the NATO ASI Series book series (volume 34)

Summary

The analysis of chromosomal deletions overlapping at the albino locus in the mouse has led to the postulation of a number of loci in this region essential for viability. One of these, the perinatal survival locus, is thought to be important for the differentiation of the liver since its deletion results in the deficiency of a set of liver-specific enzymes and ultrastructural abnormalities in the hepatocyte. By differential cDNA screening we have isolated and examined the expression of a panel of mRNAs influenced by deletion of the locus. This has been done in an attempt to elucidate the role of the normal gene product. In addition, as a prelude to cloning the perinatal survival locus, a long-range restriction map has been established onto which the extents of albino locus deletions are being placed.

Keywords

Serine Polypeptide Dexamethasone Glucocorticoid Fibrinogen 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Gavin D. Kelsey
    • 1
  • Siegfried Ruppert
    • 1
  • Michael Burchart
    • 1
  • Andreas Schedl
    • 1
  • Wolfgang Schmid
    • 1
  • Günther Schütz
    • 1
  1. 1.Institut für Zell- und Tumorbiologie Deutsches KrebsforschungszentrumHeidelbergFederal Republic of Germany

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