Developmental Regulation and Modulation of Neuronal Nicotinic Acetylcholine Receptor Channels

  • Linda K. Simmons
  • Brenda L. Moss
  • Stephen M. Schuetze
  • Lorna W. Role
Conference paper
Part of the NATO ASI Series book series (volume 25)


To date the best characterized receptor-channel complex is the muscle nicotinic acetylcholine receptor (AChR). Several studies demonstrate that muscle AChRs are subject to various forms of regulation. Distinct changes in channel properties, receptor distribution and subunit composition are observed over the course of development. Also, acute changes in the degree of receptor phosphorylation induce a functional change in the rate of receptor desensitization (for a review, see Schuetze and Role 1987). These extensive studies of muscle AChR regulation provide an important intellectual framework for studies of other ligand-gated channels in the central and peripheral nervous system.


Single Channel Sympathetic Neuron Patch Pipette Amplitude Histogram Single Channel Activity 
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  1. Albuquerque, E., Deshpande, S., Aracava, Y., Alkondon, M. and Daly, J.W. (1986) A possible involvement of cyclic AMP in the expression of desensitization of the nicotinic acetylcholine receptor. A study with forskolin and its analogs. F.E.B.S. Lett. 199:113–120CrossRefGoogle Scholar
  2. Clapham, D. E. and Neher, E. (1984) Substance P reduces acetylcholine-induced currents in isolated bovine chromaffin cells. J. Physiol. 347: 255–277PubMedGoogle Scholar
  3. Downing, J. D., Harish, O. H., and Role, L. W. (1987) Modulation of neuronal acetylcholine receptor desensitization by ganglionic peptides and agents that activate protein kinase C. Soc. Neurosci. Abs. 13: 704Google Scholar
  4. Downing, J. D. and Role, L. W. (1987) Activators of protein kinase C enhance acetylcholine receptor desensitization in sympathetic ganglion neurons. PNAS 84: 7739–7743PubMedCrossRefGoogle Scholar
  5. Downing, J. D. and Role, L. W. Desensitization of the neuronal acetylcholine receptor: characterization and modulation by ganglionic peptides (In preparation)Google Scholar
  6. Eusebi, F., Grassi, C, Nervi, C, Caparale, S., Adamo, B. and Molinaro, M. (1987) Acetylcholine may regulate its own nicotinic receptor-channel through the C-kinase system. Proc. Roy. Soc. Lond. B 230:355–365CrossRefGoogle Scholar
  7. Hamill, O. P., Marty, A., Neher, E., Sakmann, B. and Sigworth, F. J. (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv 391, 85–100PubMedCrossRefGoogle Scholar
  8. Hayashi, M., Edgar, D. and Thoenen, H. (1983) The development of substance P, somatostatin and vasoactive intestinal polypeptide in sympathetic and spinal sensory ganglia of the chick embryo. Neurosci. 10:31–39CrossRefGoogle Scholar
  9. Huganir, R. L., Delcour, A. H., Greengard, P. and Hess, G. P. (1986) Phosphorylation of the nicotinic acetylcholine receptor regulates its rate of desensitization. Nature 321: 774–776PubMedCrossRefGoogle Scholar
  10. Margiotta, J. F. and Berg, D. K. (1986) Enkephalin and substance P modulate synaptic properties of chick ciliary ganglion neurons in cell culture. Neurosci. 18: 175–182CrossRefGoogle Scholar
  11. Middleton, P., Jaramillo, F. and Schuetze, S. M. (1986) Forskolin increases the rate of acetylcholine receptor desensitization at rat soleus endplates. PNAS 83: 4967–4971PubMedCrossRefGoogle Scholar
  12. New, H.V. and Mudge, A.W. (1986) Distribution and ontogony of SP, CGRP, SOM, and VIP in chick sensory and sympathetic ganglia. Dev. Biol. 116:337–346PubMedCrossRefGoogle Scholar
  13. Role, L. W. (1984) Substance P modulation of acetylcholine-induced currents in embryonic chicken sympathetic and ciliary ganglion neurons. PNAS 81: 2924–2928PubMedCrossRefGoogle Scholar
  14. Role, L. W. (1988) Neural regulation of acetylcholine sensitivity in embryonic sympathetic neurons. PNAS 85: 2925–2829CrossRefGoogle Scholar
  15. Sakmann, B., Patlak, J., and Neher, E. (1980) Single acetylcholine-activated channels show burst-kinetics in presence of desensitizing concentrations of agonist. Nature 286:71–73PubMedCrossRefGoogle Scholar
  16. Schuetzé, S. M. and Role, L. W. (1987) Developmental regulation of nicotinic acetylcholine receptors. Ann. Rev. Neuroci. 10: 403–457CrossRefGoogle Scholar
  17. Valenta, D. C. C, Downing, J. D. and Role, L. W. (1987) Peptidergic regulation of catecholamine secretion in embryonic sympathetic neurons. Soc. Neurosci. Abs. 13: 702Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • Linda K. Simmons
    • 1
  • Brenda L. Moss
    • 1
    • 2
  • Stephen M. Schuetze
    • 1
  • Lorna W. Role
    • 1
  1. 1.Depts of Anatomy and Cell Biology in the Center for Neurobiology and BehaviorColumbia University College of Physicians of SurgeonsNYUSA
  2. 2.Dept of Biological SciencesColumbia UniversityNYUSA

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