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Distribution of [3H]N-Methylcarbamylcholine Binding Sites in Rat Brain: Correlation with Nicotinic Autoreceptors

  • P. A. Lapchak
  • D. M. Araujo
  • R. Quirion
  • B. Collier
Part of the NATO ASI Series book series (volume 25)

Abstract

Nicotinic receptors in the mammalian brain have been studied using a variety of radioligands including [3H]acetylcholine (Schwartz et al., 1982; Schwartz and Kellar, 1983), [3H]nicotine (Clarke et al., 1985) and [125I]-α-bungarotoxin (Clarke et al., 1986). These compounds have been shown to bind to multiple recognition sites in the CNS, one of which may be a high-affinity nicotinic receptor (Clarke et al., 1985). More recently, rat brain cortical nicotinic receptors have been characterized using [3H]N-methylcarbamylcholine ([3H]-MCC) (Boksa and Quirion, 1987). This novel nicotinic ligand binds specifically, saturably and with high affinity to a single class of binding sites in rat brain homogenates (Abood and Grassi, 1986; Boksa and Quirion, 1987); thus, it is thought to be a superior ligand to study the brain nicotinic receptor. The pattern of ligand specificity for the displacement of [3H]MCC suggested that this ligand bound to high affinity nicotinic receptors (Boksa and Quirion, 1987).

Keywords

Frontal Cortex Nicotinic Receptor Chronic Nicotine Striatal Slice Nicotinic Cholinergic Receptor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. A. Lapchak
    • 1
    • 3
  • D. M. Araujo
    • 2
    • 3
  • R. Quirion
    • 2
    • 3
  • B. Collier
    • 1
    • 3
  1. 1.Department of PharmacologyMcGill UniversityMontrealCanada
  2. 2.Department of PsychiatryMcGill UniversityMontrealCanada
  3. 3.Douglas Hospital Research CenterVerdunCanada

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