Distribution of [3H]N-Methylcarbamylcholine Binding Sites in Rat Brain: Correlation with Nicotinic Autoreceptors
Nicotinic receptors in the mammalian brain have been studied using a variety of radioligands including [3H]acetylcholine (Schwartz et al., 1982; Schwartz and Kellar, 1983), [3H]nicotine (Clarke et al., 1985) and [125I]-α-bungarotoxin (Clarke et al., 1986). These compounds have been shown to bind to multiple recognition sites in the CNS, one of which may be a high-affinity nicotinic receptor (Clarke et al., 1985). More recently, rat brain cortical nicotinic receptors have been characterized using [3H]N-methylcarbamylcholine ([3H]-MCC) (Boksa and Quirion, 1987). This novel nicotinic ligand binds specifically, saturably and with high affinity to a single class of binding sites in rat brain homogenates (Abood and Grassi, 1986; Boksa and Quirion, 1987); thus, it is thought to be a superior ligand to study the brain nicotinic receptor. The pattern of ligand specificity for the displacement of [3H]MCC suggested that this ligand bound to high affinity nicotinic receptors (Boksa and Quirion, 1987).
KeywordsFrontal Cortex Nicotinic Receptor Chronic Nicotine Striatal Slice Nicotinic Cholinergic Receptor
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