Signals and Structures Involved in Early Interactions between Plants and Viruses or Pseudoviruses
One mechanism by which stable plant RNA virus particles might be disassembled in vivo involves recruitment of cell factors and ribosomes by a short, easily exposed portion of RNA. No cell specificity exists during virus uptake or early cotranslational disassembly. Virus-like reporter ribonucleocapsids were synthesized in vitro to study the mechanism, sequence-dependence and site of disassembly and transient gene expression without concomitant replication. “Pseudoviruses” and transcapsidated viral RNAs can elucidate the mechanism(s) of genetically engineered, coat protein-mediated cross-protection in electroporated tobacco protoplasts or plants.
KeywordsCoat Protein Tobacco Mosaic Virus Mesophyll Protoplast Cowpea Chlorotic Mottle Virus Wheat Germ Extract
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