Signals and Structures Involved in Early Interactions between Plants and Viruses or Pseudoviruses

  • T. Michael A. Wilson
  • Kitty A. Plaskitt
  • John W. Watts
  • Jane K. Osbourn
  • Peter A. C. Watkins
Part of the NATO ASI Series book series (volume 41)

Abstract

One mechanism by which stable plant RNA virus particles might be disassembled in vivo involves recruitment of cell factors and ribosomes by a short, easily exposed portion of RNA. No cell specificity exists during virus uptake or early cotranslational disassembly. Virus-like reporter ribonucleocapsids were synthesized in vitro to study the mechanism, sequence-dependence and site of disassembly and transient gene expression without concomitant replication. “Pseudoviruses” and transcapsidated viral RNAs can elucidate the mechanism(s) of genetically engineered, coat protein-mediated cross-protection in electroporated tobacco protoplasts or plants.

Keywords

Starch Codon Fractionation Polypeptide Hull 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • T. Michael A. Wilson
    • 1
  • Kitty A. Plaskitt
    • 1
  • John W. Watts
    • 1
  • Jane K. Osbourn
    • 1
  • Peter A. C. Watkins
    • 1
  1. 1.John Innes InstituteAFRC Institute of Plant Science ResearchNorwich, NorfolkUK

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