The Use and Misuse of Viruses in Cloning and Expression in Plants

  • Roger Hull
Conference paper
Part of the NATO ASI Series book series (volume 41)

Abstract

Plant viruses have attracted considerable attention as possible vectors for introducing “foreign” genes into plants. Two basic systems have been studied: integration of viral sequences into the host chromosome, and use as episomal vectors. Integrated promoter sequences from DNA viruses, primarily the cauliflower mosaic virus 35S promoter, have been used successfully. Episomal vectors have been constructed both from DNA and RNA viral sequences for both the transient and the constitutive expression of genes; some major problems remain to be fully overcome. The use of viral sequences in plants, whether as gene vectors or to confer non-conventional forms of resistance, raises the possibility that there may be risks on the field release of modified plants.

Keywords

Maize Codon Recombination Polyethylene Glycol 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Roger Hull
    • 1
  1. 1.John Innes InstituteAFRC Institute of Plant Science ResearchNorwichUK

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