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Putative Ethylene Binding Protein(S) from Abscission Zones of Phaseolus Vulgaris

  • Cathal P. Connern
  • A. R. Smith
  • R. Turner
  • M. A. Hall
Part of the NATO ASI Series book series (volume 35)

Abstract

Ethylene binding sites exist in abscission zones of Phaseolus vulgaris (Hall el al., 1987). However, attempts to characterise these binding sites have been unsuccessful due to both the low concentrations of binding sites present in a zone two cells thick and the low maximum specificity of 14Cethylene. An alternative approach has now been taken using an ethylene binding protein (EBP) purified from developing cotyledons of Phaseolus vulgaris as previously described by Thomas et al. (1985) and Williams (1988). Polyclonal antibodies (Anti-EBP) were raised against the EBP and characterised in terms of immune response, sensitivity and specificity (Western blotting) using a colloidal gold labelled goat-anti-rabbit secondary antibody. Antibodies, specific to the EBP were used to probe abscission zones from the same tissue. In this paper, evidence will be presented for the existence of putative EBP (cross reacting antigens) in abscission zones from Phaseolus vulgaris. However, the nature and biological function of these proteins is unknown at present.

Keywords

Colloidal Gold Integral Membrane Protein Abscission Zone Silver Enhancement Petiole Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Hall MA, Howarth CJ, Robertson D, Sanders IO, Smith AR, Smith PG, Starling RJ, Tang Z-D, Thomas CJR, Williams RAN (1987) Ethylene binding proteins. In: Jackson MB, Fox JE (eds) Molecular biology of plant growth control, Alan R Liss, New York, pp 335–344Google Scholar
  2. Osborne DJ, McManus MT (1985) Target cells for ethylene action. In: Roberts JA, Tucker GA (eds) Ethylene and plant development. Butterworths, London, p 197Google Scholar
  3. Thomas CJR, Smith AR, Hall MA (1985) Partial purification of an ethylene binding site from Phaseolus vulgaris L. cotyledons. Planta 164: 272–277CrossRefGoogle Scholar
  4. Williams R (1988) Purification of an ethylene binding protein from Phaseolus vulgaris L. cotyledons. PhD Thesis, University College of Wales, AberystwythGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Cathal P. Connern
    • 1
  • A. R. Smith
    • 1
  • R. Turner
    • 1
  • M. A. Hall
    • 1
  1. 1.Department of Biological SciencesUniversity College of WalesAberystwyth, DyfedWales, UK

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