Putative Ethylene Binding Protein(S) from Abscission Zones of Phaseolus Vulgaris
Ethylene binding sites exist in abscission zones of Phaseolus vulgaris (Hall el al., 1987). However, attempts to characterise these binding sites have been unsuccessful due to both the low concentrations of binding sites present in a zone two cells thick and the low maximum specificity of 14Cethylene. An alternative approach has now been taken using an ethylene binding protein (EBP) purified from developing cotyledons of Phaseolus vulgaris as previously described by Thomas et al. (1985) and Williams (1988). Polyclonal antibodies (Anti-EBP) were raised against the EBP and characterised in terms of immune response, sensitivity and specificity (Western blotting) using a colloidal gold labelled goat-anti-rabbit secondary antibody. Antibodies, specific to the EBP were used to probe abscission zones from the same tissue. In this paper, evidence will be presented for the existence of putative EBP (cross reacting antigens) in abscission zones from Phaseolus vulgaris. However, the nature and biological function of these proteins is unknown at present.
KeywordsColloidal Gold Integral Membrane Protein Abscission Zone Silver Enhancement Petiole Tissue
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