Regulation, maturation and function of tomato fruit polygalacturonase

  • Alan B. Bennett
  • Dean DellaPenna
  • Robert L. Fischer
  • James Giovannoni
  • James Lincoln
Part of the NATO ASI Series book series (volume 35)

Abstract

Ripening represents a major transition in tomato fruit development. This developmental transition is manifested by alterations in metabolism that contribute to final fruit colour, flavour and texture. It has been proposed, and a great deal of supporting evidence advanced, that these alterations in metabolism result from specific alterations in gene expression (Briggs et al., 1986; DellaPenna et al., 1986; Lincoln et al., 1987; Mansson et al., 1985; Maunders et al., 1987; Rattanapanone et al., 1978; Speirs et al., 1984).

Keywords

Chlorophyll Propylene Polypeptide Hemicellulose Pectin 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ali ZM, Brady CJ (1982) Purification and characterization of the polygalacturonase of tomato fruits. Aust J. Plant Physiol 9:155-169Google Scholar
  2. Baldwin EA, Pressey R (1988) Tomato polygalacturonase elicits ethylene production in tomato fruit. J Amer Soc Hort Sci 113:92–95Google Scholar
  3. Bennett, AB, DellaPenna D (1987a) Polygalacturonase gene expression in ripening tomato fruit. In: D Nevins, R Jones (ed) Tomato biotechnology, pp 299–308Google Scholar
  4. Bennett AB, DellaPenna D (1987b) Polygalacturonase: its importance and regulation in ripening. In: WW Thompson, E Nothnagle, R Huffaker (eds) Plant senescence: Its biochemistry and physiology. Amer Soc of Plant Physiol, Rockville, pp 98–107Google Scholar
  5. Biggs MS, Harriman RW, Handa AK (1986) Changes in gene expression during tomato fruit ripening. Plant Physiol 81:395–403PubMedCrossRefGoogle Scholar
  6. Brady CJ (1987) Fruit ripening. Annu Rev Plant Physiol 38:155–178.CrossRefGoogle Scholar
  7. Brady CJ, MacAlpine G, McGlasson WB, Ueda Y (1982) Polygalacturonase in tomato fruits and the induction of ripening. Aust J Plant Physiol 9:171–178CrossRefGoogle Scholar
  8. Crookes PR, Grierson D (1983) Ultrastructure of tomato fruit ripening and the role of polygalacturonase isoenzymes in cell wall degradation. Plant Physiol 72:1088–1093PubMedCrossRefGoogle Scholar
  9. DellaPenna D, Alexander DC, Bennett AB (1986) Molecular cloning of tomato fruit polygalacturonase: Analysis of polygalacturonase mRNA levels during ripening. Proc Natl Acad Sci USA 83:6420–6424PubMedCrossRefGoogle Scholar
  10. DellaPenna D, Lincoln J, Fischer R, Bennett AB (1989) Transcriptional analysis of polygalacturonase and other ripening-associated genes in normal and mutant tomato fruit. Plant Physiol (in press)Google Scholar
  11. DellaPenna D, Bennett AB (1988) In vitro synthesis and processing of tomato fruit polygalacturonase. Plant Physiol 86:1057–1063PubMedCrossRefGoogle Scholar
  12. Giovannoni J, DellaPenna D, Bennett AB, Fischer R (1989) Expression of a chimeric polygalacturonase gene in transgenic rin fruit results in pectin degradation but not fruit softening. Plant Cell 1:53–63PubMedCrossRefGoogle Scholar
  13. Grierson D, Tucker GA, Keen J, Ray J, Bird CR, Schuch W (1986) Sequencing and identification of a cDNA clone for tomato polygalac turonase. Nucl Acids Res 14:8595–8603PubMedCrossRefGoogle Scholar
  14. Hobson GE (1964) Polygalacturonase in normal and abnormal tomato fruit. Biochem J 92:324–332PubMedGoogle Scholar
  15. Hobson DJ (1965) The firmness of tomato fruit in relation of polygalacturonase activity. J Hort Sci 40:66–72Google Scholar
  16. Huber DJ (1981) Polyuronide degradation and hemicellulose modifications in ripening tomato fruit. J Amer Soc Hort Sci 108:405–409Google Scholar
  17. Huber DJ (1983) The role of cell wall hydrolases in fruit softening. Hort Rev 5:169–219Google Scholar
  18. Lincoln JE, Cordes S, Read E, Fischer RL (1987) Regulation of gene expression by ethylene during tomato fruit development. Proc Natl Acad Sci USA 84:2793–2797PubMedCrossRefGoogle Scholar
  19. Mansson PE, Hsu D, Stalker D (1985) Characterization of fruit specific cDNAs from tomato. Mol Gen Genet 200:356–361CrossRefGoogle Scholar
  20. Maunder MJ, Holdsworth MJ, Slater A, Knapp JE, Bird CR, Schuch W, Grierson D (1987) Ethylene stimulates the accumulation of ripening-related mRNAs in tomatoes. Plant Cell and Environ 10:177–184Google Scholar
  21. Rattanapanone N, Spiers J, Grierson D (1978) Evidence for changes in messenger RNA content related to tomato fruit ripening. Phytochem 17:1485–1486CrossRefGoogle Scholar
  22. Sato T, Kusaba S, Nakagawa H, Ogura N (9184) Cell free synthesis of a putative precursor of polygalacturonase in tomato fruits. Plant and Cell Physiol 25:1069–1071Google Scholar
  23. Sheehy RE, Pearson J, Brady CJ, Hiatt WR (1987) Molecular characterization of tomato fruit polygalacturonase. Mol Gen Genet 208:30–36CrossRefGoogle Scholar
  24. Sheehy RE, Kramer M, Hiatt WR (1988) Reduction of polygalacturonase activity in tomato fruit by antisense RNA. Proc Natl Acad Sci USA 85:8805–8809PubMedCrossRefGoogle Scholar
  25. Smith CJS, Watson CF, Ray J, Bird CR, Morris PC, Schuch W, Grierson D (1988) Antisense RNA inhibition of polygalacturonase gene expression in transgenic tomatoes. Nature 334:724–726CrossRefGoogle Scholar
  26. Speirs J, Brady CJ, Grierson D, Lee E (1984) Changes in ribosome organization and mRNA abundance in ripening tomato fruit. Aust J Plant Physiol 11:225–233CrossRefGoogle Scholar
  27. Themmen APN, Tucker GA, Grierson D (1982) Degradation of isolated tomato cell walls by purified polygalacturonase in vitro. Plant Physiol 69:122–124PubMedCrossRefGoogle Scholar
  28. Tucker D (1982) Synthesis of polygalacturonase during tomato fruit ripening. Planta 155:64–67CrossRefGoogle Scholar
  29. Wallner SJ, Bloom HL (1977) Characteristics of tomato cell wall degradation in vitro. Implication for the study of fruit-softening enzymes. Plant Physiol 60:207–210PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Alan B. Bennett
    • 1
  • Dean DellaPenna
    • 1
  • Robert L. Fischer
    • 1
    • 2
  • James Giovannoni
    • 1
    • 2
  • James Lincoln
    • 1
    • 2
  1. 1.Mann Laboratory Department of Vegetable CropsUniversity of CaliforniaDavisUSA
  2. 2.Division of Molecular Plant BiologyUniversity of CaliforniaBerkeleyUSA

Personalised recommendations