Transdifferentiation to a Neuronal Phenotype in Adult Bovine Chromaffin Cells: Effects of αMSH, bFGF and Histamine

  • Barbara A. Demeneix
  • Nikolai Kley
Conference paper
Part of the NATO ASI Series book series (volume 32)


The effects of histamine, basic fibroblast growth factor (bFGF) and a melanocyte stimulating hormone (αMSH) on neurite outgrowth, proenkephalin A (pENK) and tyrosine hydroxylase (TH) gene expression have been studied in primary cultures of chromaffin cells from the adult bovine adrenal medulla. All three factors promote transdifferentiation to the neuronal phenotype (assessed by neurite outgrowth) but histamine acts with a much shorter latency than bFGF and αMSH. Moreover, only histamine produces any modification of pENK gene expression at the times examined (8,24,48 and 72h). Possible intracellular signalling mechanisms linked to transdifferentiation in bovine chromaffin cells are discussed.


PC12 Cell Nerve Growth Factor Tyrosine Hydroxylase Neurite Outgrowth Chromaffin Cell 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Barbara A. Demeneix
    • 1
  • Nikolai Kley
    • 1
  1. 1.Department of NeuropharmacologyMax-Planck-Institut für PsychiatriePlanegg-MartinsriedGermany

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