Skip to main content
SpringerLink
Log in
Book cover

Molecular Biology of Neuroreceptors and Ion Channels pp 519–540Cite as

Myotube Specific Expression of the Mouse AChR Delta Subunit Gene is Conferred by 148 BP of 5′ Flanking DNA

  • Conference paper

  • 112 Accesses

Part of the book series: NATO ASI Series ((ASIH,volume 32))

Abstract

The skeletal muscle AChR is a ligand-gated channel composed of four structurally related subunits. The four subunits assemble into a heteropentamer (alpha2, beta, gamma and delta) which binds acetylcholine and thus initiates a permeability change in the myofiber membrane that ultimately couples release of neurotransmitter to myofiber contraction (Fambrough, 1979; Karlin, 1980; Anderson, 1987).

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Anderson, D.J. 1987. Molecular biology of the acetylcholine receptor: structure and regulation of biogenesis. In: The Vertebrate Neuromuscular Junction. M.M. Salpeter, ed. Alan R. Liss, Inc. N.Y., N.Y. 285–315.

    Google Scholar 

  • Baldwin, T.J., C.M. Yoshihara, K. Blackmer, C.R. Kintner and S.J. Burden. 1988. Regulation of acetylcholine receptor transcript expression during development in Xenopus laevis. J. Cell Biol. 106: 469–478.

    Article  PubMed  CAS  Google Scholar 

  • Berg, D.K., R.B. Kelly, P.B. Sargent, P. Williamson and Z.W. Hall. 1972. Binding of alpha-bungarotoxin to acetylcholine receptors in mammalian muscle. Proc. Natl. Acad. Sci. USA. 69: 147–151.

    Article  PubMed  CAS  Google Scholar 

  • Bergsma, D.J., J.M. Grichnik, L.M. Gossett and R.J. Schwartz. 1986. Delimitation and characterization of cis-acting DNA sequences required for the regulated expression and transcriptional control of the chicken skeletal alpha-actin gene. Mol. Cell. Biol. 6: 2462–2475.

    PubMed  CAS  Google Scholar 

  • Billeter, R., W. Quitschke and B.M. Patterson. 1988. Approximately 1 kilobase of sequence 5’ to the two myosin light-chain lf/3f gene cap sites is sufficient for differentiation-dependent expression. Mol. Cell. Biol. 8: 1361–1365.

    PubMed  CAS  Google Scholar 

  • Bullock, P., J. Miller and M. Botchan. 1986. Effects of poly [d(pGpT)-d(pApC)] and poly [d(pCpG)-d(pCpG)] repeats on homologous recombination in somatic cells. Mol. Cell. Biol. 6: 3948–3953.

    PubMed  CAS  Google Scholar 

  • Burden, S.J. 1977. Development of the neuromuscular junction in the chick embryo: the number, distribution and stability of acetylcholine receptors. Dev. Biol. 57: 317–329.

    Article  PubMed  CAS  Google Scholar 

  • Chirgwin, J.M., A.E. Przybyla, R.J. MacDonald and W.J. Rutter. 1979. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. 18: 5294–5299.

    Article  PubMed  CAS  Google Scholar 

  • Crowder, C.M. and J. P. Merlie. 1986. DNase I-hypersensitive sites surround the mouse acetylcholine receptor delta-subunit gene. Proc. Natl. Acad. Sci. USA. 83: 8405–8409.

    Article  PubMed  CAS  Google Scholar 

  • Evans, S., Goldman, D., S. Heinemann and J. Patrick. 1987. Muscle acetylcholine receptor biosynthesis: regulation by transcript availability. J. Biol. Chem. 262: 4911–4916.

    PubMed  CAS  Google Scholar 

  • Fambrough, D.M. 1979. Control of acetylcholine receptors in skeletal muscle. Physiol. Rev. 59: 165–227.

    PubMed  CAS  Google Scholar 

  • Frischauf, A.M., H. Lehrach, A. Poustka and N. Murray. 1983. Lambda replacement vectors carrying polylinker sequences. J. Mol. Biol. 170: 827–842.

    Article  PubMed  CAS  Google Scholar 

  • Gorman, C.M., L.F. Moffat and B.H. Howard. 1982. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Mol. Cell. Biol. 2: 1044–1051.

    PubMed  CAS  Google Scholar 

  • Grichnik, J.M., D.J. Bergsma and R.J. Schwartz. 1986. Tissue restricted and stage specific transcription is maintained within 411 nucleotides flanking the 5’ end of the chicken alpha-skeletal actin gene. Nucleic. Acids Res. 14: 1683–1701.

    Article  PubMed  CAS  Google Scholar 

  • Hamada, N., M. Seidman, B. H. Howard and C.M. Gorman. 1984. Enhanced gene expression by the poly (dT-dG)-poly (dC-dA) sequence. Mol. Cell. Biol. 4: 2622–2630.

    PubMed  CAS  Google Scholar 

  • Heidmann, O., A. Buonanno, B. Geoffroy, B. Robert, J.L. Guenet, J.P. Merlie and J.P. Changeux. 1986. Chromosomal localization of muscle nicotinic acetylcholine receptor genes in the mouse. Science. 234: 866–868.

    Article  PubMed  CAS  Google Scholar 

  • Hu, M.C.T. S.B. Sharp and N. Davidson. 1986. The complete sequence of the mouse skeletal alpha-actin gene reveals several conserved inverted repeat sequences outside the protein coding region. Mol. Cell. Biol. 6: 15–25.

    PubMed  Google Scholar 

  • Karlin, A. 1980. Molecular properties of nicotinic acetylcholine receptors. In: The Cell Surface and Neuronal Function. C. Cotman, G. Post, and G. Nicolson, eds. Elsevier/North Holland. 191–260.

    Google Scholar 

  • Klarsfeld, A. and J.P. Changeux. 1985. Activity regulates the levels of acetylcholine receptor alpha-subunit mRNA in cultured chicken myotubes. Proc. Natl. Acad. Sci. USA. 82: 4558–4562.

    Article  PubMed  CAS  Google Scholar 

  • Klarsfeld, A., P. Daubas, B. Bourachot and J.P. Changeux. 1987. A 5’-flanking region of the chicken acetylcholine receptor alpha-subunit gene confers tissue specificity and developmental control of expression in transfected cells. Mol. Cell. Biol. 7: 951–955.

    PubMed  CAS  Google Scholar 

  • Konieczny, S.F. and C.P. Emerson. 1987. Complex regulation of the muscle- specific contractile protein (troponin I) gene. Mol. Cell. Biol. 7: 3065–3075.

    PubMed  CAS  Google Scholar 

  • Laimins, L.A., G. Khoury, C. Gorman, B. Howard and P. Gruss. 1982. Host- specific activation of gene expression of 72 base pair repeats of Simian virus 40 and Moloney murine leukemia virus. Proc. Natl. Acad. Sci. USA. 79: 6453–6457.

    Article  PubMed  CAS  Google Scholar 

  • LaPolla, R.J., K. Mixter-Mayne and N. Davidson. 1984. Isolation and characterization of a cDNA clone for the complete protein coding region of the delta subunit of the mouse acetylcholine receptor. Proc. Natl. Acad. Sci. USA. 81: 7970–7974.

    Article  PubMed  CAS  Google Scholar 

  • Lomo, T. and R.H. Westgaard, 1975. Control of ACh senstivity in rat muscle fibers. Cold Spring Harbor Symp. Quant. Biol. 40: 263–274.

    Google Scholar 

  • Maniatis, T., E.F. Fritsch and J. Sambrook. 1982. Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.

    Google Scholar 

  • Melton, D.A., P.A. Krieg, M.R. Rebagliati, K. Zinn and M. Green. 1984. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12: 7035–7056.

    Article  PubMed  CAS  Google Scholar 

  • Merlie, J.P. K.E. Isenberg, S.D. Russell and J.R. Sanes. 1984. Denervation supersensitivity in skeletal muscle: analysis with a cloned cDNA probe. J. Cell Biol. 99: 332–335.

    Article  PubMed  CAS  Google Scholar 

  • Miledi, R. and L.T. Potter. 1971. Acetylcholine receptors in muscle fibers. Nature. 233: 599–603.

    Article  PubMed  CAS  Google Scholar 

  • Nef, P., A. Mauronf R. Stalder, C. Alliod and M. Ballivet. 1984. Structure, linkage, and sequence of the two genes encoding the delta and gamma subunits of the nicotinic acetylcholine receptor. Proc. Natl. Acad. Sci. USA. 81: 7975–7979.

    Article  PubMed  CAS  Google Scholar 

  • Noda, M., H. Takahashi, T. Tanabe, M. Toyosato, S. Kikyotani, T. Hirose, M. Asai, H. Takashima, S. Inayama, T. Miyata and S. Numa. 1983. Structural homology of Torpedo californica acetylcholine receptor subunits. Nature, 302: 528–532.

    Article  PubMed  CAS  Google Scholar 

  • Patterson, B. and J. Prives. 1973. Appearance of acetylcholine receptor in differentiating cultures of embryonic chick breast muscle. J. Cell Biol. 59: 241–245.

    Article  Google Scholar 

  • Salpeter, M.M. 1987. Development and neural control of the neuromuscular junction and of the junctional acetylcholine receptor. In: The Vertebrate Neuromuscular Junction. M.M. Salpeter, ed. Alan R. Liss, Inc., N.Y. 55–115.

    Google Scholar 

  • Sanger, F., S. Nicklen and A.R. Coulson. 1977. DNA sequencing with chain termination inhibitors. Proc. Natl. Acad. Sci. USA. 74: 5463–5467.

    Article  PubMed  CAS  Google Scholar 

  • Selden, R.F., K.B. Howie, M.E. Rowe, H.M. Goodman and D.D. Moore. 1986. Human growth hormone as a reporter gene in regulation studies employing transient gene expression. Mol. Cell. Biol. 6: 3173–3179.

    PubMed  CAS  Google Scholar 

  • Shirakata, M., Y. I. Nabeshima, K. Konishi, and Y. Fujii-Kuriyama. 1988. Upstream regulatory region for inducible expression of the chicken skeletal myosin alkali light-chain gene. Mol. Cell. Biol. 8: 2581–2588.

    PubMed  CAS  Google Scholar 

  • Southern, P.J. and P. Berg. 1982. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J. Mol. Appl. Gen. 1: 327–341.

    CAS  Google Scholar 

  • Treco, D. and N. Arnheim. 1986. The evolutionarily conserved repetitive sequence d(TG-AC)n promotes reciprocal exchange and generates unusual recombinant tetrads during yeast meiosis. Mol. Cell. Biol. 6: 3934–3947.

    PubMed  CAS  Google Scholar 

  • Wigler, M., A. Pellicer, S. Silverstein, R. Axel, G. Urlaub and L. Chasin. 1979. DNA mediated transfer of the adenine phospho-ribosyltransferase locus into mammalian cells. Proc. Natl. Acad. Sci. USA. 76: 1373–1376.

    Article  PubMed  CAS  Google Scholar 

  • Yu, L., R. J. LaPolla and N. Davidson. 1986. Mouse muscle nicotinic acetylcholine receptor gamma subunit: cDNA sequence and gene expression. Nucleic Acids Res. 14: 3539–3555.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biology Department, 16-820, Massachusetts Institute of Technology, Cambridge, Massachusetts, 02139, USA

    T. J. Baldwin & S. J. Burden

Authors
  1. T. J. Baldwin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. S. J. Burden
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Ernährungsphysiologie, Rheinlanddamm 201, 4600, Dortmund, Germany

    Alfred Maelicke

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Baldwin, T.J., Burden, S.J. (1989). Myotube Specific Expression of the Mouse AChR Delta Subunit Gene is Conferred by 148 BP of 5′ Flanking DNA. In: Maelicke, A. (eds) Molecular Biology of Neuroreceptors and Ion Channels. NATO ASI Series, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74155-5_42

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-74155-5_42

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74157-9

  • Online ISBN: 978-3-642-74155-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation