Abstract
Glutathione is involved in the protection of phagocytes against oxidative damage and in the assembly and preservation of microtubules during phagocytosis (Voetmann et al 1980). The phagocytotic activity may be impaired by depletion of GSH in phagocytes. This may lead to a failure of unspecific and specific defense mechanisms, such as the clearance of microparticles from blood by Kupffer cells (Altura 1980), which are resident macrophages in the liver. In the present study the blood clearance of microparticles in rats was used to investigate the effects of GSH-depleting electrophilic substances on the phagocytosis in vivo.
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Abbreviations
- CC:
-
Colloidal carbon
- DEM:
-
Diethyl maleate
- GSH:
-
Reduced glutathione
- MMS:
-
Tc-99m-labeled millimicrospheres of human serum albumin
References
Altura BM (1980) Reticuloendothelial cells and host defense. Adv Microcirc 9: 252–294
Hennighausen G (1986) Effects of diethyl maleate on non-protein sulfhydryl content and cellular functions of mouse thymocytes in vitro. Arch Toxicol Suppl 9: 297–301
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Sedlak J, Lindsay RH (1968) Estimation of total protein-bound and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25: 192–205
Voetman AA, Loos JA, Roos D (1980) Changes in the levels of glutathione in phagocytosing human neutrophiles. Blood 55: 741–747
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© 1989 Springer-Verlag Berlin Heidelberg
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Hennighausen, G., Engel, M. (1989). Depletion of Hepatic Glutathione in Rats Impairs Phagocytosis In Vivo . In: Chambers, P.L., Chambers, C.M., Greim, H. (eds) Biological Monitoring of Exposure and the Response at the Subcellular Level to Toxic Substances. Archives of Toxicology, vol 13. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74117-3_62
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DOI: https://doi.org/10.1007/978-3-642-74117-3_62
Publisher Name: Springer, Berlin, Heidelberg
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