Depletion of Hepatic Glutathione in Rats Impairs Phagocytosis In Vivo

  • G. Hennighausen
  • M. Engel
Conference paper
Part of the Archives of Toxicology book series (TOXICOLOGY, volume 13)


Glutathione is involved in the protection of phagocytes against oxidative damage and in the assembly and preservation of microtubules during phagocytosis (Voetmann et al 1980). The phagocytotic activity may be impaired by depletion of GSH in phagocytes. This may lead to a failure of unspecific and specific defense mechanisms, such as the clearance of microparticles from blood by Kupffer cells (Altura 1980), which are resident macrophages in the liver. In the present study the blood clearance of microparticles in rats was used to investigate the effects of GSH-depleting electrophilic substances on the phagocytosis in vivo.


Human Serum Albumin Kupffer Cell Blood Clearance Resident Macrophage Hepatic Glutathione 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Colloidal carbon


Diethyl maleate


Reduced glutathione


Tc-99m-labeled millimicrospheres of human serum albumin


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Altura BM (1980) Reticuloendothelial cells and host defense. Adv Microcirc 9: 252–294Google Scholar
  2. Hennighausen G (1986) Effects of diethyl maleate on non-protein sulfhydryl content and cellular functions of mouse thymocytes in vitro. Arch Toxicol Suppl 9: 297–301PubMedCrossRefGoogle Scholar
  3. Orrenius S (1985) Biochemical mechanisms of cytotoxicity, TIPS, FEST - Suppl 15–20Google Scholar
  4. Sedlak J, Lindsay RH (1968) Estimation of total protein-bound and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25: 192–205PubMedCrossRefGoogle Scholar
  5. Voetman AA, Loos JA, Roos D (1980) Changes in the levels of glutathione in phagocytosing human neutrophiles. Blood 55: 741–747PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • G. Hennighausen
    • 1
  • M. Engel
    • 1
  1. 1.Institute of Pharmacology and ToxicologyWilhelm-Pieck-University of RostockRostockGermany

Personalised recommendations