Insulin pp 169-181 | Cite as

Insulin Receptor Structure

  • R. A. Roth
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 92)

Abstract

The insulin receptor serves to not only concentrate insulin on the appropriate target cells, but also to initiate the responses of these cells to the hormone. Consequently, a great deal of research has been focused on this molecule. Early efforts were directed at quantitating the interaction of radioactively labeled insulin with its receptor (for reviews of these studies see GAMMELTOFT 1984; ROTH and GRUNFELD 1981 ; KAHN 1976). From these studies it was possible to
  1. 1

    Estimate the number of insulin receptors on different cells under various physiological conditions;

     
  2. 2

    Show that the interaction of insulin with its receptor results in a subsequent decrease in the number of cell surface receptors (the phenomenon called “downregulation”);

     
  3. 3

    Show that the interaction of insulin with its receptors is not a simple one since Scatchard plots of binding data were curvilinear, a phenomenon which has been interpreted to mean that insulin was interacting with two populations of receptors or that there was one class of receptors which exhibits negative cooperativity;

     
  4. 4

    Show that the rank order of potency of different insulin analogs varies in their binding to insulin receptors in different tissues, suggesting that the insulin receptor might differ in these tissues.

     

Keywords

Urea Carbohydrate Tyrosine Cysteine Electrophoresis 

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© Springer-Verlag Berlin Heidelberg 1990

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  • R. A. Roth

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