Abstract
Disturbances of growth are very common in the human epidermis, and there is mounting evidence that epidermal growth processes can be modified by the action of certain pharmacological agents; consequently, in many instances, investigators may find themselves in the situation where a measurement of proliferative rate in epidermis, possibly of the human, is mandatory. The question then arises, which measurement to make, and how to interpret the results? It is the purpose of this chapter to offer advice on both of these facets.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Aherne WA, Camplejohn RS, Wright NA (1977) An introduction to cell population kinetics. Arnold, London
Al-Mukhtar MYT, Polak J, Bloom SR, Wright NA (1982) The search for appropriate measurements of proliferative and morphological status in studies of intestinal adaptation. In: Robinson JWL, Dowling RH, Riecken EO (eds) Mechanisms of intestinal adaptation. MTP Press, Lancaster, pp 3–28
Bauer FW, de Grood RM (1975) Impulse cytophotometry in psoriasis. Br J Dermatol 93:225–228
Bauer FW, Crombag NHMN, de Grood RM, de Jongh GJ (1980) Flow cytometry as a tool for the study of cell kinetics in epidermis. Br J Dermatol 102:629–639
Bauer FW, Crombag NHCMN, Boezemann JBM, de Grood RM (1981) Flow cytometry as a tool for the study of cell kinetics in skin. 2. Cell kinetic data for psoriasis. Br J Dermatol 104:271–276
Burholt DR, Schultze B, Maurer W (1976) Mode of growth of the jejunal crypt cells of the rat; an autoradiographic study using double labelling with 3H and 14C-thymidine in lower and upper parts of the crypts. Cell Tissue Kinet 9:107–117
Camplejohn RS (1983) Kinetic measurements in normal human epidermis in vivo. In: Wright NA, Camplejohn RS (eds) Psoriasis: cell proliferation. Livingstone, Edinburgh, pp 163–172
Camplejohn RS, Gelfant S, Chalker D (1981) An attempt to use vincristine and colcemid to measure proliferative rates in normal human epidermis in vivo. Br J Dermatol 104:243–248
Colamonici OR, Trepei JB, Neckers LM (1985) Phorbol ester enhances deoxynucleoside incorporation while inhibiting proliferation of K562 cells. Cytometry 6:591–596
Dolbeare F, Gratzner H, Pallavicini MG, Gray JW (1983) Flow cytometric measurement of total DNA content and incorporated bromodeoxyuridine. Proc Natl Acad Sci USA 80:5573–5577
Duffill M, Wright NA, Shuster S (1976) The cell proliferation kinetics of psoriasis examined by three in vivo techniques. Br J Dermatol 94:355–362
Evensen A (1962) Significance of the mitotic duration in evaluating the kinetics of cellular proliferation. Nature 195:718–719
Fisher LB (1968) Determination of the normal rate and duration of mitosis in human epidermis. Br J Dermatol 80:24–28
Frentz G, Moller U, Christensen I (1980) DNA flow cytometry on human epidermis. I. Methodological studies on normal skin. J Invest Dermatol 74:119–121
Gelfant S, Drewinko B, Chalker D, Eisinger M (1983) Cycling and non-cycling human ger-minative epidermal cells — the continuous 3H-thymidine labelling method in vivo and flow cytometric studies. In: Wright NA, Camplejohn RS (eds) Psoriasis: cell proliferation. Livingstone, Edinburgh, pp 209–217
Goodwin PG, Hamilton S, Fry L (1974) The cell cycle time in psoriasis. Br J Dermatol 90:517–524
Gray JW, Carver JH, George YS, Mendelsohn MC (1977) Rapid cell cycle analysis by measurement of the radioactivity per cell in a narrow window in S phase (RCS). Cell Tissue Kinet 10:97–109
Grove GL (1979) Epidermal cell kinetics in psoriasis. Int J Dermatol 18:111–122
Laurence EB, Spargo DJ, Thornley AL (1979) Cell proliferation kinetics of epidermis and sebaceous glands in relation to chalone action. Cell Tissue Kinet 12:615–633
Marks R (1975) Is there a relationship between clinical morphology and epidermal cell kinetics? Proc R Soc Med 68:161–167
Marks R, Dykes PJ (1983) Steroids, squamous epithelium and psoriasis. In: Wright NA, Camplejohn RS (eds) Psoriasis: cell proliferation. Livingstone, Edinburgh, pp 327–335
Maurer HR (1981) Potential pitfalls of [3H] thymidine techniques to measure cell proliferation. Cell Tissue Kinet 14:111–120
Maurer HR, Laerum OD (1976) Granulocyte chalone testing: a critical review. In: Houck JC (ed) Chalones. North-Holland, Amsterdam, pp 331–350
Pullman H, Lennartz KJ, Steigleder GK (1974) In vitro estimation of cell proliferation in normal and psoriatic epidermis with special regard to diurnal variations. Arch Dermatol Forsch 250:177–184
Ralf IG (1983) The metaphase arrest method in human epidermis. In: Wright NA, Camplejohn RS (eds) Psoriasis: cell proliferation. Livingstone, Edinburgh, pp 80–92
Ralfs IG, Dawber R, Ryan T, Duffill M, Wright NA (1981) The kinetics of metaphase arrest in psoriatic human epidermis; an examination of optimal experimental conditions for determining the birth rate. Br J Dermatol 104:231–242
Schultze B, Maurer W, Hagenbusch H (1976) A two emulsion atuoradiographic technique and the discrimination of the three different types of labelling after double labelling with 3H and 14C thymidine. Cell Tissue Kinet 9:245–255
Tvermyr EMF (1972) Circadian rhythms in hairless mouse epidermal DNA-synthesis as measured by double labelling with 3H thymidine (H3TdR). Virchows Arch [B] 11:43–54
Vorhees JJ (1976) Cyclic AMP and cyclic GMP in epidermal physiology and pathophysiology. Curr Probl Dermatol 6:107–153
Weinstein GD, Frost P (1968) Abnormal cell proliferation in psoriasis. J Invest Dermatol 50:257–262
Wright NA (1983) Statistical and operational problems in cell kinetic methodology in human epidermis. In: Wright NA, Camplejohn RS (eds) Psoriasis: cell proliferation. Livingstone, Edinburgh, pp 117–123
Wright NA, Alison MR (1984) The biology of epithelial cell populations. Clarendon, Oxford
Wright NA, Appleton DR (1980) The metaphase arrest method — a critical review. Cell Tissue Kinet 13:643–663
Yanagisawa M, Dolbeare F, Todoroki T, Gray JW (1985) Cell cycle analysis using numerical simulations of bivariate DNA/bromodeoxyuridine distributions. Cytometry 6:550–562
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Dover, R., Wright, N.A. (1989). Methods for the Study of Proliferative Rates in Epidermis. In: Greaves, M.W., Shuster, S. (eds) Pharmacology of the Skin II. Handbook of Experimental Pharmacology, vol 87 / 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74054-1_1
Download citation
DOI: https://doi.org/10.1007/978-3-642-74054-1_1
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-74056-5
Online ISBN: 978-3-642-74054-1
eBook Packages: Springer Book Archive