MHC + X pp 186-192 | Cite as

Analysis of Specificities Recognized by Monoclonal Antibodies and Cytotoxic T-Lymphocytes Using Chimeric H-2 Molecules

  • J. F. Abastado
  • J. Maryanski
  • C. Jaulin
  • P. Kourilsky
Conference paper

Abstract

In order to develop structure-function studies on H-2 molecules, several groups (reviewed by Forman 1987) have taken advantage of the fact that the different domains of H-2 are encoded by separate exons. Chimeric molecules have been built in which a given domain of an H-2 antigen has been replaced by the corresponding domain of another H-2 antigen; this approach, dubbed “exon shuffling,” has permitted the assignment to a particular domain of the specificities recognized by monoclonal antibodies (McAbs). However, for the specificities recognized by alloreactive or syngeneic cytotoxic T-Iymphocytes (CTLs) this approach has yielded rather limited information: whereas in most cases it could be shown that the specificity of a given CTL is carried by the first two external domains, shuffling of these two domains usually destroyed the recognition by CTLs.

Keywords

Recombination Resi Fluores Kanamycin 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • J. F. Abastado
    • 1
  • J. Maryanski
    • 2
  • C. Jaulin
    • 1
  • P. Kourilsky
    • 1
  1. 1.Unité de Biologie Moléculaire du Gène, U277 de l’Institut National de la Santé et de la Recherche Médicale, UA 535 du Centre National de la Recherche Scientifique, Dpt. d’immunologieInstitut PasteurParisFrance
  2. 2.Ludwig Institute for Cancer Research, Lausanne BranchChemin des BoveressesEpalingesSwitzerland

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