Analysis of Specificities Recognized by Monoclonal Antibodies and Cytotoxic T-Lymphocytes Using Chimeric H-2 Molecules
In order to develop structure-function studies on H-2 molecules, several groups (reviewed by Forman 1987) have taken advantage of the fact that the different domains of H-2 are encoded by separate exons. Chimeric molecules have been built in which a given domain of an H-2 antigen has been replaced by the corresponding domain of another H-2 antigen; this approach, dubbed “exon shuffling,” has permitted the assignment to a particular domain of the specificities recognized by monoclonal antibodies (McAbs). However, for the specificities recognized by alloreactive or syngeneic cytotoxic T-Iymphocytes (CTLs) this approach has yielded rather limited information: whereas in most cases it could be shown that the specificity of a given CTL is carried by the first two external domains, shuffling of these two domains usually destroyed the recognition by CTLs.
KeywordsExon Shuffling Chimeric Molecule Recombination Point Polymorphic Residue Separate Exon
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