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Synthetic, Multi-Deuteriated Cholesterol as a Quantitative Probe of the Formation of Cholesterol Oxidation Products

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Natural Products Chemistry III

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Abstract

The synthesis of 24, 24, 25, 26, 26, 26, 27, 27, 27 - 2H9 - cholesterol is described. A method for use of the compound as an internal monitor for determination of the origin of oxidation products of cholesterol and their quantitation in naturally occurring media is discussed. In the analytical procedure the labeled cholesterol is added to the sample immediately upon collection thus subjecting the labeled and unlabeled substrates to identical oxidation conditions during the analyte isolation process and corrects for procedural artifacts. Conventional capillary gas chromatography-flame ionization detection (GC-FID) and combined gas chromatography-mass spectrometry (GC-MS) have been used. Quantitation with an accuracy of 5% or better at the low part per million level for some of the common oxidation products is demonstrated.

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Author information

Authors and Affiliations

  1. Department of Chemistry and Barnett Institute of Chemical Analysis, Northeastern University, Boston, MA, 02115, USA

    B. A. Wasilchuk, P. Feibush, P. W. Le Quesne & Paul Vouros

Authors
  1. B. A. Wasilchuk
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  2. P. Feibush
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  3. P. W. Le Quesne
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  4. Paul Vouros
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Editor information

Editors and Affiliations

  1. HEJ Research Institute of Chemistry, University of Karachi, Karachi-32, Pakistan

    Atta-ur-Rahman

  2. Department of Chemistry, Northeastern University, 360 Huntington Avenue, Boston, MA, 02115, USA

    Philip William Le Quesne

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© 1988 Springer-Verlag Berlin Heidelberg

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Wasilchuk, B.A., Feibush, P., Le Quesne, P.W., Vouros, P. (1988). Synthetic, Multi-Deuteriated Cholesterol as a Quantitative Probe of the Formation of Cholesterol Oxidation Products. In: Atta-ur-Rahman, Le Quesne, P.W. (eds) Natural Products Chemistry III. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74017-6_15

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  • DOI: https://doi.org/10.1007/978-3-642-74017-6_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74019-0

  • Online ISBN: 978-3-642-74017-6

  • eBook Packages: Springer Book Archive

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