Mac-1+ Bone Marrow Cells Include Precursors of B Cells and T Cells
Previous studies of lymphomas and normal cells suggested that the putatively macrophage-specific antigen, Mac-1 may be expressed by precursors of the B cell lineage. This possibility was tested by analyzing the potential of sorted Mac-1+ cells from adult bone marrow (BM) to reconstitute CB.17-scid mice or sublethally irradiated B6-Ly-5.2 congenic mice. The results show that Mac-1+ BM cells include precursors to both T and B lymphocytes as determined by FACS analysis of spleen, bone marrow and thymus from mice inoculated with Mac-1+ cells. Three color FACS analysis showed that thymocyte subpopulations from B6-congenic mice inoculated with Mac-1+ cells were composed largely of donor cells, indicating that the donor-derived peripheral T cells had undergone normal thymic processing.
KeywordsDonor Cell FACS Analysis Scid Mouse Congenic Mouse Radiation Chimera
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