Delivery of c-myc Antisense Phosphorothioate Oligodeoxynucleotides to Hematopoietic Cells in Culture by Liposome Fusion: Specific Reduction in c-myc Protein Expression Correlates with Inhibition of Cell Growth and DNA Synthesis

  • S. L. Loke
  • C. Stein
  • X. Zhang
  • M. Avigan
  • J. Cohen
  • L. M. Neckers
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 141)

Abstract

To understand the role of individual genes in the regulation of growth and differentiation, their specific inhibition is necessary. When the gene products are surface proteins, such as growth factor receptors, such inhibition may be accomplished by use of monoclonal antibodies capable of interfering with the binding of ligand to receptor (Neckers and Cossman 1983). However, when the protein of interest is localized to an intracellular compartment, antibodies must be delivered by microinjection to one cell at a time. While possible, such methodology is tedious, requires specialized apparatus and techniques, and does not lend itself to the study of cells growing in suspension culture (i.e., most hematopoietic cells).

Keywords

Codon Leukemia Polyethylene Glycol Explosive 

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References

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1988

Authors and Affiliations

  • S. L. Loke
    • 1
  • C. Stein
    • 2
  • X. Zhang
    • 1
  • M. Avigan
    • 1
  • J. Cohen
    • 2
  • L. M. Neckers
    • 1
  1. 1.Laboratory of PathologyNCI, NIHBethesdaUSA
  2. 2.Medicine BranchNCI, NIHBethesdaUSA

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