Delivery of c-myc Antisense Phosphorothioate Oligodeoxynucleotides to Hematopoietic Cells in Culture by Liposome Fusion: Specific Reduction in c-myc Protein Expression Correlates with Inhibition of Cell Growth and DNA Synthesis
To understand the role of individual genes in the regulation of growth and differentiation, their specific inhibition is necessary. When the gene products are surface proteins, such as growth factor receptors, such inhibition may be accomplished by use of monoclonal antibodies capable of interfering with the binding of ligand to receptor (Neckers and Cossman 1983). However, when the protein of interest is localized to an intracellular compartment, antibodies must be delivered by microinjection to one cell at a time. While possible, such methodology is tedious, requires specialized apparatus and techniques, and does not lend itself to the study of cells growing in suspension culture (i.e., most hematopoietic cells).
KeywordsHL60 Cell Leukemia Cell Line HL60 Promyelocytic Leukemia Cell Line Promyelocytic Leukemia Cell Line HL60 Phosphorothioate Oligodeoxynucleotides
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- Dash, P, Lotan, I, Knapp, M, Kandel, ER, Goelet, P (1987) Selective elimination of mRNAs in vivo: Complementary oligodeoxynucleotides promote RNA degradation by an RNase H — like activity. Proc. Natl. Acad. Sci. USA 84:7896–7900 progress from GO to Gl. Nature 328:445–449Google Scholar
- Heikkila, R, Schwab, G, Wickstrom, E, Loke, SL, Pluznik, D, Watt, R, Neckers, LM (1987) A c-myc antisense oligodeoxynucleotide inhibits entry into S phase but notGoogle Scholar
- Papahadjopoulos, D, Vail, WJ, Jacobson, K, Poste, G (1975) Cochleate lipid cylinders: formation by fusion of unilamellar lipid vesicles. Biochim. Biophys. Acta 394:483–491Google Scholar
- Stein, CA, Cohen, JS (in press) Oligodeoxynucleotides: Towards a new chemotherapy. Cancer Res.Google Scholar
- Stein, CA, Mori, K, Loke, SL, Subasinghe, C, Shinozuka, K, Cohen, JS, Neckers, LM (in press) Phosphorothioate and normal oligodeoxynucleotides with 5′ — linked acridine: Characterization and preliminary Kinetics of of cellular uptake. GeneGoogle Scholar