Altered Growth Phenotype of a Burkitt’s Lymphoma Line Following the Introduction and Stable Expression of the EBNA 2A Gene
Using recombinant retrovirus-mediated transfer, the coding region for the Epstein-Barr virus-encoded EBNA 2A gene was selectively introduced and stably expressed in the Louckes Burkitt lymphoma cell line. Transfected cells displayed the following altered growth characteristics when compared with the parental line: (i) maintenance of proliferation at reduced serum concentrations, (ii) improved seeding efficiency, (iii) production of a unique growth-enhancing factor, (iv) increased sensitivity to the unique factor. These growth-related changes were accompanied by the appearance of large amounts of soluble CD23 fragments in medium conditioned by Louckes cells expressing EBNA 2A. Tumor-promoting phorbol esters were found to induce similar phenotypic change in the Louckes parental line as seen on the introduction of the EBNA 2A gene. Possible mechanisms by which EBNA 2A provides a growth advantage to B lymphoma cells are discussed.
KeywordsPhorbol Myristic Acetate Burkitt Lymphoma Latent Membrane Protein Soluble CD23 Final 16hr
Unable to display preview. Download preview PDF.
- Cairns J A, Flores-Romo L, Millsum M J, Guy G R, Ledbetter J A, Gillis S, Gordon J (1988) Soluble CD23 is released by B lymphocytes cycling in response to interleukin 4 and anti-Bp50 (CDw40) Eur. J. Immunol. (In press).Google Scholar
- Gregory C D, Murray R J, Edwards C J, Rickinson A B (1988) Down-regulation of cell adhesion molecules LFA-3 and ICAM-1 in Epstein-Barr virus-positive Burkitt’s lymphoma underlines tumour cell escape from virus-specific T cell surveillance. J. Exp. Med. (In press).Google Scholar