Abstract
The development of transgenic mice carrying specific cancer-promoting genes represents a major advance in molecular oncology (for reviews, see Cory and Adams, 1988; Hanahan, 1987). In principle, the effect of a particular oncogene can be assessed for every cell type within the animal by this approach. The transgene can either be linked to a catholic regulatory sequence and expressed throughout all tissues, or its expression can be confined to a particular cell lineage by fusing it to a tissue-specific promoter/enhancer control element. Our own interests center on hematopoietic neoplasia, so we have produced transgenic mice harboring oncogenes targeted for constitutive expression in hematopoietic cells. The principal regulatory sequence we have used is the immunoglobulin (Ig) heavy chain enhancer (Eµ). While the function of this sequence is to direct expression of Ig heavy chain (IgH) genes in B lymphoid cells, it may also be active in at least some T lymphoid and even myeloid cells since the IgH locus is transcriptionally active in certain T and myeloid cell lines (Kemp et al, 1980a) and thymocytes (Kemp et al, 1980b) and an immunoglobulin µ transgene can be expressed in T as well as B cells (Grosschedl et al, 1984).
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© 1988 Springer-Verlag Berlin · Heidelberg
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Harris, A.W. et al. (1988). Transgenic Mouse Models for Hematopoietic Tumorigenesis. In: Potter, M., Melchers, F. (eds) Mechanisms in B-Cell Neoplasia 1988. Current Topics in Microbiology and Immunology, vol 141. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74006-0_12
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DOI: https://doi.org/10.1007/978-3-642-74006-0_12
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