Abstract
The structure of the g-actin monomer complexed with DNaseI has been solved by x-ray crystallography to 0.45nm resolution, Fig.l (Kabsch, Mannherz, & Suck, 1985). In the following we describe the structure of f-actin arrived at by a search procedure: the structure obtained from crystallography at 0.6nm is placed in all possible orientations in the F-actin helix; the fibre diffraction pattern is computed from the resulting structure and compared with the x-ray diffraction data from orientated gels of F-actin (Popp, Lednev, & Jahn, 1986) measured to 0.8 nm resolution. This process yielded five possible solutions at low resolution (2.0nm) only one of which successfully refined to high resolution (0.8nm). A full account of this study is in preparation (Holmes, et al. 1989). The best of the five possible solutions resulting from the low resolution search is shown in Fig. 2. The (intensity) R-factor is 0.12. To refine at high resolution we adopted an iterative least squares procedure. However, no solution would refine satisfactorily. The resulting R-factors ranged from 0.37–0.45.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Aebi, U., Millonig, R., Salvo, H., & Engel, A. (1986) The three- dimensional structure of the actin filament revisited. Annal. New York Acad. Sci 483, 100–119.
Amos, L. A., Huxley, H. E., Holmes, K. C., Goody, R. S., & Taylor, K. A. (1982) Structural evidence that myosin heads may interact with two sites on F-actin. Nature (Lond.) 5882, 467–469.
Egelman, E. H. & DeRosier, D. J. (1983). Structural studies of F- actin. in Actin: Its structure and function on muscle and non- muscle cells (ed. dos Remedios & Barden) pp 17–24. Academic Press London.
Holmes, K. C., Popp, D., Gebhard, W., Kabsch, W., Jahn, W., & Bryan,R. (1989) The structure of f-actin as revealed by analysis of the x- ray fibre diffraction pattern, (in preparation)
Kabsch, W., Mannherz, H. G. & Suck, D. (1985) Three dimensional structure of the complex of actin and DNase I at 4.5 A resolution. EMBO Journal 4, 2113–2118.
Milligan, R. A. & Flicker, P.F. (1987) Structural relationships of actin, myosin, and tropomyosin revealed by cryo-electron microscopy. J. Cell Biology 105, 29–39
O’Brien, E. J., Couch, J., Johnson, G. R. P., & Morris, E. P. (1983) Structure of actin and the thin filament, in Actin: Its structure and function on muscle and non-muscle cells (ed. dos Remedios & Barden ) pp 3–16. Academic Press, London.
Popp, D., Lednev, V. V., & Jahn, W. (1987) Methods of preparing well-orientated sols of f-actin containing filaments suitable for x-ray diffraction. J. molec. Biol. 197, 679–684
Sutoh, K. (1982) Identification of myosin binding sites on the actin sequence. Biochemistry 21, 3654–3661
Taylor, D. L., Reidler, J., Spudich, J. A., & Stryer, L. (1981) Detection of actin assembly by fluorescence energy transfer. J. Cell Biology 89, 362–367.
Trinick, J., Cooper, J., Seymour, J., & Egelman, E.H. (1986) Cryoelectron microscopy and three-dimensional reconstruction of actin filaments. J. of Microscopy 141, 349–360.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Holmes, K.C., Popp, D., Gebhard, W., Kabsch, W. (1989). The Structure of F-Actin Calculated from X-Ray Fibre Diagrams and the 0.6 nm Crystal Structure. In: Aebi, U., Engel, J. (eds) Cytoskeletal and Extracellular Proteins. Springer Series in Biophysics, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73925-5_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-73925-5_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73927-9
Online ISBN: 978-3-642-73925-5
eBook Packages: Springer Book Archive