Monoclonal Antibody 127 Recognizes a Subpopulation of Chicken Hexabrachion/Tenascin

  • V. A. Lightner
  • C. Pegram
  • D. Bigner
  • H. P. Erickson
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 3)

Abstract

Hexabrachion/tenascin is an extracellular matrix protein with a distinct spatial and temporal distribution in tissue (Erickson and Lightner, 1988). In chicken embryo fibroblast cultures it is isolated as a hexameric protein with disulfide linked subunits of 250, 225 and 215 Kd (Chiquet and Fambrough, 1984a,b; Erickson and Lightner, 1988). These subunits have very similar proteolytic cleavage patterns, indicating they are related peptides (Chiquet and Fambrough, 1984b). Pulse chase experiments (Chiquet and Fambrough, 1984b) and recently published sequence data (Jones et al.,1988; Pearson et al.,1988) suggest they result from alternative splicing of the tenascin mRNA. However, nothing is known about the functional differences between these subunits or how they are assembled into the hexameric structure.

Keywords

Glycerol Bromide Disulfide Myeloma Cyanogen 

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References

  1. Bourdon, M.A., Wikstrand, C.J., Furthmayr, H., Matthews, T.J. and Bigner, D.D. (1983) Cancer Res. 43: 2796–2805PubMedGoogle Scholar
  2. Chiquet, M. and Fambrough, D.M. (1984a) J. Cell Biol. 98: 1937–1946PubMedCrossRefGoogle Scholar
  3. Chiquet, M. and Fambrough, D.M. (1984b) J.Cell Biol. 98: 1926–1936PubMedCrossRefGoogle Scholar
  4. Engvall, E. and Perlmann, P. (1972) J.Immunol. 109: 129–135PubMedGoogle Scholar
  5. Erickson, H.P. and Iglesias, J.L. (1984) Nature 311: 267–269PubMedCrossRefGoogle Scholar
  6. Erickson, H.P. and Lightner, V.A. (1988) Adv.Cell Biol. 2: in pressGoogle Scholar
  7. Erickson, H.P. and Taylor, H.C. (1987) J.Cell Biol. 105: 1387–1394PubMedCrossRefGoogle Scholar
  8. Jones, F.S., Burgoon, M.P., Hoffman, S., Crossin, K.L., Cunningham, B. A. and Edelman, G.M. (1988) Proc.Natl.Acad.Sci.U.S.A. 85:2186–2190PubMedCrossRefGoogle Scholar
  9. Marton, L.S., Gulcher, J.R. and Stefansson, K. (1987) J. Cell Biol. 105: 135a, (Abstract)Google Scholar
  10. Pearson, C.A., Pearson, D., Shibahara, S., Hofsteenge, J. and Chiquet-Ehrismann, R. (1988) EMBO J. 7: 2977–2982PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • V. A. Lightner
    • 1
    • 2
  • C. Pegram
    • 1
    • 2
  • D. Bigner
    • 1
    • 2
  • H. P. Erickson
    • 1
    • 2
  1. 1.Department of Cell BiologyDuke University Medical CenterDurhamUSA
  2. 2.Department of PathologyDuke University Medical CenterDurhamUSA

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