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Biosynthesis and Mode of Insertion of a Stalked Intrinsic Membrane Protein of the Small Intestinal Brush Border

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Dynamics of Membrane Proteins and Cellular Energetics

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Abstract

As revealed by electron microscopy (negative staining) the outer, luminal surface of brush border membranes is studied with lollipop-like proteins. These proteins have been identified with a number of enzymes, nearly all of them hydrolases. These proteins protrude into the lumen, and are attached to the membrane bilayer via a hydrophobic “stalk” and an embedded hydrophobic segment (hence the name of “stalked intrinsic membrane proteins” (Brunner et al., 1979) (see more below). As these enzymes of the plasma membrane exert their function on the outside of the membrane they are referred to as ectoenzymes. An extended but certainly not exhaustive list of the hydrolases of brush border membranes is given in Table 1 (for recent reviews see Kenny and Turner, 1987). Much attention has been paid in the last ten years or so to the mode of membrane insertion and biosynthesis of these proteins (for a recent review, see Semenza 1986).

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  1. G. Semenza
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Editors and Affiliations

  1. Laboratoire de Biochimie et Biologie Moléculaire, Université de Franche-Comté UA CNRS 531, 25030, Besançon Cedex, France

    Norbert Latruffe  & Yves Gaudemer  & 

  2. Laboratoire de Biochimie, CENG — DRF Biochimie UA CNRS 1130, 38041, BP 85 X, Grenoble Cedex, France

    Pierre Vignais

  3. Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstrasse 28, 3012, Bern, Switzerland

    Angelo Azzi

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Semenza, G. (1988). Biosynthesis and Mode of Insertion of a Stalked Intrinsic Membrane Protein of the Small Intestinal Brush Border. In: Latruffe, N., Gaudemer, Y., Vignais, P., Azzi, A. (eds) Dynamics of Membrane Proteins and Cellular Energetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73905-7_2

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  • DOI: https://doi.org/10.1007/978-3-642-73905-7_2

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