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Isolation of Intact Chloroplasts - Criteria of Integrity

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Dynamics of Membrane Proteins and Cellular Energetics
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Abstract

Intact chloroplasts are defined as those with an intact envelope as revealed by the ability to carry out light-dependent CO2 fixation ; ribonuclease and protease resistant RNA and protein synthesis ; starch, fatty acids and amino acids synthesis ; and to show low rates of ferricyanide reduction which are greatly increased by exposure to hypotonic conditions (for a review see Douce and Joyard, 1979).

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References

  • Bruinsma J. (1961). A comment on the spectrophotometry deter-mination of chlorophyll. Biochim. Biophys. Acta, 52: 576–578.

    Article  PubMed  CAS  Google Scholar 

  • Douce R. & Ooyard J. (1979). Structure and function of the plastid envelope. Adv. Bot. Res., 7: 1–116.

    Article  CAS  Google Scholar 

  • Edwards G. & Walker D. A. (1983). C3 C4: mechanical, and cellular and environmental regulation, of photosynthesis. Blackwell Sci. Publ. Oxford.

    Google Scholar 

  • Gunning B.E.S. & Steer M.W. (1975). Ultrastructure and the Biology of Plant Cells. Arnold, London.

    Google Scholar 

  • Heber U. & Santarius K.A. (1970). Direct and indirect transfer of ATP and ADP across the chloroplast envelope. Z. Naturforseh, 25: 718–728.

    CAS  Google Scholar 

  • Heldt H.W. (1976). Metabolite transport in intact spinach chloroplasts. in “The intact Chloroplast” (Barber 3. ed) pp. 215–234, Elsevier, Amsterdam.

    Google Scholar 

  • Jenkins C.L.D., & Russ V.J. (1984). Large scale, rapid prepa-ration of functional nesophyll chloroplasts from zea mays and other C4 species. Plant Sci. Lett., 35: 19–24.

    Article  CAS  Google Scholar 

  • Mourioux G. & Douce R. (1981). Slow passive diffusion of orthophosphate between intact isolated chloroplasts and suspending medium. Plant Physiol., 67: 470–473.

    Article  PubMed  CAS  Google Scholar 

  • Walker D.A. (1976). CO2 fixation by intact chloroplasts: Photosynthetic induction and its relation to transport phenomena and control mechanisms, in “The Intact Chloroplast” (Barber 3. ed) pp. 235–278, Elsevier, Amsterdam.

    Google Scholar 

  • Walker D.A. (1987). The use of the oxygen electrode and fluo-rescence probes in simple measurements of photosynthesis. Research institute for photosynthesis, University of Shef-field (distributed by Packard Publishing Limited, 16, Lynch Down, Funtington, Chichester, West Sussex P018 9LR).

    Google Scholar 

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© 1988 Springer-Verlag Berlin Heidelberg

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Douce, R., Joyard, J., Neuburger, M. (1988). Isolation of Intact Chloroplasts - Criteria of Integrity. In: Latruffe, N., Gaudemer, Y., Vignais, P., Azzi, A. (eds) Dynamics of Membrane Proteins and Cellular Energetics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73905-7_11

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  • DOI: https://doi.org/10.1007/978-3-642-73905-7_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-50047-6

  • Online ISBN: 978-3-642-73905-7

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