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Protein Microsequence Analysis with Dansylamino-PITC

  • Hisashi Hirano
  • Brigitte Wittmann-Liebold

Abstract

In addition to PITC introduced first for the sequential degradation of proteins and peptides by Edman (1949), several other Edman-type coupling reagents for N-terminal sequencing have been synthesized as reviewed by Doolittle (1982). However, the use of most of these reagents is associated with some limitations for sequence analysis. For example, some of them carry a bulky side group which often sterically hinders the reactive group, resulting in a low coupling yield, and some produce a large number of reaction by-products caused by the side reactions. Such problems have restricted the use of these reagents in protein sequencing.

Keywords

Phosphoenolpyruvate Carboxylase Hair Dryer Phenyl Thiohydantoin Bulky Side Group Degradation Cycle 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

DABITC

4-[4-(dimethylamino)phenylazo]phenylisothiocyanate

DABTH

4-[4-(dimethylamino)phenylazo]phenylthiohydantoin

dansylamino-PITC

4-{[5-(dimethylamino)-1-naphthylsulfonyl] amino} phenylisothiocyanate

FITC

fluoresceinisothiocyanate

HPLC

high performance liquid chromatography

PITC

phenylisothiocyanate

PTC

phenylthiocarbamoyl

PTH

phenylthiohydantoin

TLC

thin-layer chromatography

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Hisashi Hirano
    • 1
  • Brigitte Wittmann-Liebold
    • 2
  1. 1.National Institute of Agrobiological ResourcesTsukuba, IbarakiJapan
  2. 2.Abteilung WittmannMax-Planck-Institut für Molekulare GenetikDahlemWest Germany

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