The Cholinergic Binding Site: From Sequence to Function
Whereas the sequence of the nicotinic acetylcholine receptor (AChR) has been known since 1983 (Numa et al 1983), a true understanding of how this receptor functions is far from clear. In fact, this situation is quite characteristic of much of today’s molecular biology. The purification of a given protein is readily feasible, N-terminal sequencing has been impressively perfected and the subsequent steps for cloning the desired protein and the’ generation of a postulated sequence have become almost routine. Yet strategies, which allow the efficient correlation between sequence and function, are still being developed and few have been proven effective.
KeywordsPlacebo Cysteine Acetylcholine Stein Oligosaccharide
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