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The Antigenic Structure of Lysozyme as Detected by T-cells

  • Ettore Appella
  • Shuji Tanaka
  • Zoltan A. Nagy
  • Luciano Adorini

Abstract

Both T cells and B cells are capable of recognizing antigens, however, their modes of recognition differ. B cells bind protein antigens by means of their surface immunoglobulins, whereas T cells recognize antigens on the surface of an antigen presenting cell (APC) and only in association with a cell surface molecule encoded by the major histocompatibility complex (MHC) (Rosenthal 1978; Unanue 1984). Experimental evidence has been provided which shows that B cells respond to both a native and a denatured antigen, eliciting antibodies specific for each of the two forms; T cells usually do not respond to antigens in the native form, only to antigens metabolically processed by the APC (Benjamin et al 1984; Ziegler and Unanue 1981).

Keywords

Major Histocompatibility Complex Major Histocompatibility Complex Molecule Antigenic Structure Immunodominant Epitope Major Histocompatibility Complex Haplotype 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adorini L, Sette A, Buus S, Grey HM (1988) Interaction of an immunodominant epitope with la molecules in T cell activation. Proc Natl Acad Sci USA (in press)Google Scholar
  2. Allen PM, Babbitt BP, Unanue ER (1987) T cell recognition of lysozyme: the biochemical basis of presentation. Immunol Rev 98: 171–187PubMedCrossRefGoogle Scholar
  3. Allen PM, Strydom DJ, Unanue ER (1984) Processing of lysozyme by macrophage: identification of the determinant recognized by two cell hybridomas. Proc Natl Acad Sci USA 81: 2489–2493PubMedCrossRefGoogle Scholar
  4. Benjamin DC et al (1984) The antigenic structure of proteins. Ann Rev Immunol 2: 67–102CrossRefGoogle Scholar
  5. Berkower IJ, Buckenmeyer GK, Berzofsky JA (1986) Molecular mapping of a histocompatibility-restricted immunodominant epitope with synthetic and natural peptides;. Implications for T cell antigenic structure. J Immunol 136: 2498–2503PubMedGoogle Scholar
  6. Buus S, Sette A, Colon SM, Jenis DK, Grey HM (1986) Isolation and characterization of antigen-la complexes involved in T cell recognition. Cell 47: 1071–1077PubMedCrossRefGoogle Scholar
  7. Cogswell JP, Gammon G, Wilbur S, Shastri N, Miller A, Sercarz E (1988) la-dependent selection of agretopes on the partially unfolded chicken lysozyme molecule affects subsequent processing pathways and the dominance of T cell determinants. In: Pernis B (ed) Antigen Presenting Cells: Diversity, Differentiation and Regulation. Alan R Liss, NY, p 115–126Google Scholar
  8. DeLisi C, Berzofsky J (1985) T cell antigenic sites tend to be amphipathic structures. Proc Natl Acad Sci USA 82: 7048–7052PubMedCrossRefGoogle Scholar
  9. Fox BS, Chen C, Fraga E, French CA, Singh B, Schwartz RH (1987) Functionally distinct agretopic and epitopic sites. J Immunol 139: 1578–1588PubMedGoogle Scholar
  10. Gammon G, Shastri N, Cogswell J, Wilbur S, Sadegh-Nasseri S, Krzych U, Miller A and Sercarz E (1987) The choice of T-cell epitopes utilized on a protein antigen depends on multiple factors distinct from, as well as at, the determinant site. Immunol Rev 98: 51–73CrossRefGoogle Scholar
  11. Rosenthal AS (1978) Determinant selection and macrophage function in genetic control of the immune response. Immunol Rev 40: 136–152PubMedCrossRefGoogle Scholar
  12. Rothbard JB, Taylor WR (1988) A sequence pattern common to T cell epitopes. EMBO J 7:93–100PubMedGoogle Scholar
  13. Sette A, Buus S, Colon S, Smith JA, Miles C, Grey HM (1987) Structural characteristics of an antigen required for its interaction with la and recognition by T cells. Nature 328: 395–399PubMedCrossRefGoogle Scholar
  14. Sette A, Colizzi V, Appella E, Doria G, Adorini L (1986) Analysis of lysozyme-specific immune responses by synthetic peptides. Eur J Immunol 16: 1–6PubMedCrossRefGoogle Scholar
  15. Unanue ER (1984) Antigen-presenting function of the macrophage. Ann Rev Immunol 2: 395–428CrossRefGoogle Scholar
  16. Ziegler K and Unanue ER (1981) Identification of a macrophage antigen-processing event required for I-region restricted antigen presentation to lymphocytes. J Immunol 127: 1869–1880PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Ettore Appella
    • 1
  • Shuji Tanaka
    • 1
  • Zoltan A. Nagy
    • 2
  • Luciano Adorini
    • 2
  1. 1.Laboratory of Cell BiologyNational Cancer InstituteBethesdaUSA
  2. 2.Preclinical ResearchSandoz Ltd.BaselSwitzerland

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