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Cystatins and Cystatin-like Domains of Multifunctional Proteins: Solid-phase Microsequencing as a Tool to Study Structure-Function Relationships of Protein Inhibitors

  • Werner Machleidt
  • Anna Esterl
  • Irmgard Assfalg-Machleidt
  • Ulrich Thiele
  • Rudolf Vogel
  • Werner Müller-Esterl

Abstract

The cystatin superfamily has grown rapidly within the past few years. It comprises proteins that are structurally related to chicken cystatin, a small protein inhibitor of cysteine proteinases (Barrett et al 1986; Barrett 1987). Many other miniprotein inhibitors of cysteine proteinases can be grouped into family 1 (stefins) and family 2 (cystatins in a more restricted sense), and cystatin-like domains of about the same size have been found in multifunctional proteins like the kininogens (family 3).

Keywords

Cysteine Proteinase Limited Proteolysis Cyanogen Bromide Turk Versus Human Cystatin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Werner Machleidt
    • 1
  • Anna Esterl
    • 1
  • Irmgard Assfalg-Machleidt
    • 1
  • Ulrich Thiele
    • 1
  • Rudolf Vogel
    • 2
  • Werner Müller-Esterl
    • 2
  1. 1.Institut für Physiologische ChemiePhysikalische Biochemie und Zellbiologie der Universität MünchenMünchen 2Germany
  2. 2.Abteilung für Klinische Chemie und Klinische Biochemie in der Chirurgischen Klinik Innenstadt der Universität MünchenMünchen 2Germany

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