Structural Studies on Crystals of Ribosomal Particles
Ribosomes, the universal cell organelles on which protein biosynthesis takes place, consist of two subunits which associate upon initiation of the biosynthetic process. Each ribosomal subunit is a defined assembly of proteins and ribonucleic acid chains. A typical bacterial ribosome, e.g. that of Escherichia coli, has a molecular weight of 2.300.000 Dalton. Its large (50S) subunit is of 1.450.000 Dalton and consists of 33 different proteins and 2 RNA chains. The small subunit (30S) is of 850.000 Dalton and contains 21 proteins and 1 RNA chain (Wittmann, 1982).
KeywordsCrystallization Polyethylene Glycol Bacillus Polypeptide
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- Gewitz HS, Glotz C, Piefke J, YonathA, Wittmann HG (1988) Two—dimensional crystalline sheets of Bacillus stearo-thermophilus 50S ribosomal subunits containing a nascent polypeptide chain. Biochimie 70: 645–648Google Scholar
- Glotz C, Miissig J, Gewitz HS, Makowski I, Arad T, Yonath A, Wittmann HG (1987) Three—dimensional crystals of ribosomes and their subunits from eu— and archaebacteria. Biochem Internat 15: 887–895Google Scholar
- Yonath A, Leonard KR, Weinstein S, Wittmann HG (1987) Approaches to the determination of the three-dimensional architecture of ribosomal particles. Cold Spring Harbor Symposium 52: 729–741Google Scholar