Protein Sequence Analysis by Tandem Quadrupole Fourier Transform Mass Spectrometry

  • Donald F. Hunt
  • Jeffrey Shabanowitz
  • John R. YatesIII
  • Patrick R. Griffin
  • Nian Zhou Zhu

Abstract

Protein sequence analysis by tandem mass spectrometry (Hunt et al 1981) involves digestion of the sample by site specific reagents such as cyanogen bromide or proteolytic enzymes, partial fractionation of the resulting mixture of oligopeptides by microbore, reverse-phase, high-performance, liquid chromatography (HPLC) and direct analysis of peptides in each HPLC fraction by particle-bombardment, collision activated dissociation (Hunt et al 1986) or laser photodissociation (Hunt et al 1987), mass spectrometry on a multianalyzer instrument. Here we describe results obtained by laser photodissociation on a newly developed tandem quadrupole Fourier transform mass spectrometer (TQ-FTMS) (Hunt et al 1987). This instrument is shown schematically in Fig. 1A and consists of a standard ion source, two sets of quadrupole rods, an ion cyclotron resonance (ICR) cell housed within a 7 Tesla superconducting magnet, and an argon fluoride excimer laser for photodissociation of trapped ions.

Keywords

HPLC Glycerol Acetonitrile Amide Glycine 

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References

  1. Brinegar AC, Fox JE, Cooper G, Stevens A, Hauer CR, Shabanowitz J, Hunt DF (1988) Characterization of a Benzyladenine Binding Site Peptide Isolated from a Wheat Cytokinin Binding Protein: Sequence Analysis and Identification of a Single Affinity Labeled Histidine Residue by Mass Spectrometry. Proc Natl Acad Sei USA, In PressGoogle Scholar
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Donald F. Hunt
    • 1
  • Jeffrey Shabanowitz
    • 1
  • John R. YatesIII
    • 1
  • Patrick R. Griffin
    • 1
  • Nian Zhou Zhu
    • 1
  1. 1.Department of ChemistryUniversity of VirginiaCharlottesvilleUSA

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