C-terminal Sequencing: A New Look at the Schlack-Kumpf Thiocyanate Degradation Procedure
Application of the thiocyanate degradation procedure (Schlack and Kumpf 1926) to a peptide or protein (I) converts the C-terminal amino acid into a substituted thiohydantoin derivative (II) which can be subsequently cleaved to give a shortened (by one amino acid) peptide or protein (III) together with the C-terminal amino acid thiohydantoin (IV):
KeywordsHigh Performance Liquid Chromatography High Performance Liquid Chromatography Acetic Anhydride Ammonium Thiocyanate High Performance Liquid Chromatography Chromatogram
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