C-terminal Sequencing: A New Look at the Schlack-Kumpf Thiocyanate Degradation Procedure

  • Adam S. Inglis
  • John F. K. Wilshire
  • Franca Casagranda
  • Robert L. Laslett

Abstract

Application of the thiocyanate degradation procedure (Schlack and Kumpf 1926) to a peptide or protein (I) converts the C-terminal amino acid into a substituted thiohydantoin derivative (II) which can be subsequently cleaved to give a shortened (by one amino acid) peptide or protein (III) together with the C-terminal amino acid thiohydantoin (IV):

Keywords

High Performance Liquid Chromatography Proline Thiol Lysozyme Triethylamine 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Adam S. Inglis
    • 1
  • John F. K. Wilshire
    • 1
  • Franca Casagranda
    • 1
  • Robert L. Laslett
    • 2
  1. 1.Division of BiotechnologyCSIROParkvilleAustralia
  2. 2.Faculty of Applied ScienceSwinburne Institute of TechnologyHawthornAustralia

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