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Zusammenfassung

Morphometrische und stereologische Arbeiten werden innerhalb der Medizin und Biologie durchgeführt, um etwas über die quantitative Zusammensetzung des Gewebes oder der Zellen aussagen zu können. Diese Untersuchungen werden an einem Präparat, meistens an einem Schnittpräparat ausgeführt, das über teilweise komplizierte Prozeduren hergestellt wird. Der Wert der Untersuchung steht und fällt mit dem Grad, in dem die Resultate den Verhältnissen in vivo entsprechen.

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Literatur

  1. Aherne WA, Dunnill MS (1982) Morphometry. Edward Arnold, London

    Google Scholar 

  2. Amsler B, Mihatsch MJ (1977) Spezialfärbungen an Epon-Semidünn-Schnitten für die Lichtmikroskopie. Schweiz Z Med Techn Lab Pers 4: 424

    Google Scholar 

  3. Böhm N, Sandritter W (1975) DNA in tumors: a cytophotometric study. Curr Top Pathol 60: 151

    PubMed  Google Scholar 

  4. Burck H (1973) Histologische Technik. Leitfaden für die Herstellung mikroskopischer Präparate in Unterricht und Praxis. Thieme, Stuttgart

    Google Scholar 

  5. Culling CFA (1963) Handbook of Histopathological Techniques. Butterworths, London

    Google Scholar 

  6. Drijver JS, Boon ME (1983) Manipulating the Papanicolaou staining method. Role of acidity in the EA counterstain. Acta Cytol 27: 693

    Google Scholar 

  7. Everette James A (1983) The newer imaging procedures in radiological sciences. Choices of informational content and image quality. NRCP Proceedings 4: 45

    Google Scholar 

  8. Fossa SD (1975) Feulgen DNA-values in transitional cell carcinoma of the human urinary bladder. Beitr Path 155: 44

    CAS  Google Scholar 

  9. Fossa SD, Kaalhus O (1977) Computer assisted image analysis of Feulgen-stained cell nuclei from transitional cell carcinoma of the human urinary bladder. Acta Pathol Microbiol Scand Sect A 85: 590

    CAS  Google Scholar 

  10. Geyer G (1973) Ultrahistochemie. VEB Gustav Fischer Verlag, Jena

    Google Scholar 

  11. Gundersen HJG, Osterby R (1981) Optimizing sampling efficiency of stereological studies in biology: Or “Do more less well“. J Microsc 121: 65

    Google Scholar 

  12. Gundersen HJG, Boysen M, Reith A (1981) Comparison of semiautomatic, digitizer tablet and simple point counting performance in morphometry. Virchows Arch (Cell Pathol) 37: 317

    Article  Google Scholar 

  13. Gupta M, Mayhew TM, Bedi KS, Sharma AK, White FH (1983) Interanimal variation and its influence on the overall precision of morphometric estimates based on nested sampling designs. J Microsc 131: 147

    Article  PubMed  CAS  Google Scholar 

  14. Haug H (1980) The significance of quantitative stereologic experimental procedures in pathology. Pathol Res Pract 166: 144

    Article  PubMed  CAS  Google Scholar 

  15. Heitz PU (1982) Immunocytochemistry. Theory and application. Acta Histochem 25 (suppl) 17

    CAS  Google Scholar 

  16. Helander KG (1983) Thickness variations within individual paraffin and glycol methacrylate sections. J Microsc 132: 223

    PubMed  CAS  Google Scholar 

  17. Holmes AH (1927) Petrographic Methods and Calculations. Murby, London

    Google Scholar 

  18. Mayhew TM, White FH, Gohari K (1982) Towards economy of effort in quantitative ultrastructural pathology: Efficient sampling schemes for studying experimental carcinogenesis. J Pathol 138: 179

    Google Scholar 

  19. McDowell EM, Trump BF (1976) Histologic fixatives suitable for diagnostic light and electron microscopy. Arch Pathol Lab Med 100: 405

    PubMed  CAS  Google Scholar 

  20. Polak JM, Van Noorden S (eds) (1983) Immunocytochemistry. Practical Applications in Pathology and Biology. Wright PSG, Bristol

    Google Scholar 

  21. Polak JM, Varndell IM (eds) (1984) Immunolabelling for Electronmicroscopy. Elsevier, Amsterdam

    Google Scholar 

  22. Reith A, Barnard T, Rohr HP (1976) Stereology of cellular reaction patterns. CRC Crit Rev Toxicol 4: 219

    PubMed  CAS  Google Scholar 

  23. Reith A (1979) Enzyme topology and morphometry. In: Schmidt E, Schmidt FW, Trautschold I, Friedel R (eds) Advances in Clinical Enzymology. Karger, Basel, 53

    Google Scholar 

  24. Reith A, Mayhew TM (1980) Caveat bei der morphometrisch-stereologischen Bestimmung von Biomembranen. Gegenbauers morph Jahrb, Leipzig 126, 206

    Google Scholar 

  25. Reith A, Kraemer M, Vassy J (1984) The influence of mode of fixation, type of fixative and vehicles on the same rat liver: a morphometric/stereologic study by light and electron microscopy. Scanning Electron Microscopy II: 645

    Google Scholar 

  26. Reith A, Mayhew TM (eds) (1985) Stereology and Morphometry in Electron Microscopy. An Illustration of Problems and Solutions. Hemisphere Publishing Corporation, New York

    Google Scholar 

  27. Rigaut JP (1983) Image analysis in electron microscopy. In: Johannessen JV (ed) Electron Microscopy in Human Medicine. Vol I lb, McGraw-Hill, New York, 199

    Google Scholar 

  28. Rigaut JP, Margules S, Boysen M, Chalumeau M, Reith A (1982) Karyometry of pseudostratified, metaplastic and dysplastic nasal epithelium by morphometry and stereology. I. A general model for automated image analysis of epithelia. Pathol Res Pract 174: 342

    Google Scholar 

  29. Rigaut JP, Berggren P, Robertson B (1983) Automated techniques for the study of lung alveolar stereological parameters with the IBAS image analyser on optical microscopy sections. J Microsc 130: 53

    Article  PubMed  CAS  Google Scholar 

  30. Romeis B (1948) Mikroskopische Technik. R Oldenburg, München

    Google Scholar 

  31. Sandritter W, Thomas C (1977) Histopathologie. FK Schattauer Verlag, Stuttgart

    Google Scholar 

  32. Sternberger LA (1979) Immunocytochemistry. Theory and Application. 2nd edn, Wiley, New York

    Google Scholar 

  33. Thiebaut F, Rigaut JP, Feren K, Reith A (1984) Smooth-surfaced controland transformed C3H/10T-1/2 cells differ in cytology: a study by secondary electron, backscattered electron and image analysis. Scanning Electron Microscopy 1 II. 1249

    Google Scholar 

  34. Thiebaut F, Rigaut JP, Reith A (1984) A new method for easy staining of cell junctions by silver nitrate in light and electron microscopy. Mikroskopie (Wien) 41: 155

    Google Scholar 

  35. Thiebaut F, Rigaut JP, Reith A (1984) Improvement in the specificity of the silver staining technique for AgNOR-associated acidic proteins in paraffin sections. Stain Technol 59: 181

    PubMed  CAS  Google Scholar 

  36. Thompson SW, Hunt RD (1963) Selected Histochemical and Histopathological Techniques. Butterworths, London

    Google Scholar 

  37. Vanroelen Ch, Vakaet L (1984) The influence of tissue handling before fixation on the morphology of the chick blastoderm. J Microsc 134, 173

    Article  PubMed  CAS  Google Scholar 

  38. Weibel ER (1979) Stereological Methods. Vol 1: Practical Methods for Biological Morphometry. Academic Press, London

    Google Scholar 

  39. Wenzelides K, Korek G, Voss K (1981) Bestimmung des relativen RNA-Gehaltes von Leberzellkernen bei der Färbung mit Gallocyanin-Chromalaun. Acta Histochem 69: 307

    PubMed  CAS  Google Scholar 

  40. Wenzelides K, Hillman I, Voss K (1982) Zur Darstellung der Leberzellgrenzen mit Anilinblau und ihre vollautomatische Erfassung mit einem Bildanalysesystem. Acta Histochem 70: 193

    PubMed  CAS  Google Scholar 

  41. Williams MA (1977) Autoradiography and immunocytochemistry. In: Glauert AM (ed) Practical Methods in Electron Microscopy. Vol 6, Part I, North Holland, Amsterdam, 1

    Google Scholar 

  42. Zimmerman HW (1983) Physikalisch-chemische Grundlagen der Färbung für manuelle und apparative Zytodiagnostik. In: Wittekind D (ed) Manuelle und automatisierte Zytodiagnostik. Microscopica Acta Suppl 6: 45

    Google Scholar 

  43. Bahr GF, Bibbo M, Oehme M, Puls J, Frank R, Reale MD, Wied GL (1978) An automated device for the production of cell preparations suitable for automatic assessment. Acta Cytol 22: 243

    PubMed  CAS  Google Scholar 

  44. Burger G, Jutting U, Soost HJ (1983) Notwendigkeit und Aufbau von zytologischen Datenbanken. Microsc Acta (Suppl 6 ): 103

    CAS  Google Scholar 

  45. Eason PJ, Tucker JH (1979) The preparation of cervical scrape material for automated cytology using gallocyanin chrome-alum stain. J Histochem Cytochem 27: 25

    Article  PubMed  CAS  Google Scholar 

  46. Driel-Kulker AMJ van, Ploem-Zaaijer JJ, van der Zwan M, Tanke HJ (1980) A preparation technique for exfoliated and aspirated cells allowing different staining procedures. Analyt Quant Cytol 2: 243

    Google Scholar 

  47. Erhardt R, Reinhardt ER, Schlipf W, Bloss WH (1980) FAZYTAN: A system for fast automated cell segmentation, cell image analysis and feature extraction based on TV-image pickup and parallel processing. Analyt Quant Cytol 2: 25

    CAS  Google Scholar 

  48. Hedley DW, Friedlander ML, Taylor JW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31: 1333

    Article  PubMed  CAS  Google Scholar 

  49. Husain OAN, Page-Roberts BA, Millet JA (1978) A sample preparation for automated cervical cancer screening. Acta Cytol 22: 15

    PubMed  CAS  Google Scholar 

  50. IAC Committee on Automated Cytology (Chairman: Dorothy L Rosenthal) (1984) Specifications for automated cytodiagnostic systems proposed by the International Academy of Cytology. Analyt Quant Cytol 6: 146

    Google Scholar 

  51. Koss LG (1984) Cytochemistry. Acta Cytol 28: 353

    CAS  Google Scholar 

  52. Leif RC, Gall S, Dunlap LA, Railey C, Zucker RM, Leif B (1975) Centrifugal cytology IV: The preparation of fixed stained dispersions of gynecological cells. Acta Cytol 19: 159

    PubMed  CAS  Google Scholar 

  53. Otto K, Höffken H, Soost HJ (1979) Components and results of a new preparation technique for automated analysis of cervical samples. Analyt Quant Cytol 1: 127

    CAS  Google Scholar 

  54. Otto K, Höffken H, Soost HJ (1979) Sedimentation velocity separation: a preparation method for cervical samples. J Histochem Cytochem 27: 14

    Article  PubMed  CAS  Google Scholar 

  55. Oud PS, Zahniser DJ, Harbers-Hendriks R, van Boekel MCG, Raaijmakers, MCT, Vooijs PG (1981) The development of a cervical smear preparation procedure for the BioPEPR image analysis system. Analyt Quant Cytol 3: 73

    CAS  Google Scholar 

  56. Oud PS, Zahniser DJ, Raaijmakers MCT, Vooijs PG, van de Walle RT (1981) Thionine-FeulgenCongo red staining of cervical smears for the BioPEPR image analysis system. Analyt Quant Cytol 3: 289

    CAS  Google Scholar 

  57. Ploem JS, van Driel-Kulker AMJ, Mesker WE (1984) Preparation of monolayer smears from paraffin-embedded tissue for image cytometry of nuclear DNA content. In: Abstracts of the 13th Europ Congr of Cytol, 27. - 29. Sept 1984, Varna, pp 167

    Google Scholar 

  58. Rosenthal DL, Stern E, McLatchie C, Wu A, Lagasse LD, Wall R, Castleman KR (1979) A simple method of producing a monolayer of cervical cells for digital image processing. Analyt Quant Cytol 1: 84

    CAS  Google Scholar 

  59. Schenck U, Neugebauer D, Otto K, Soost HJ, Wagner D (1981) Monolayer preparation of cervical samples: Cytomorphologic characteristics and differential cytology. Analyt Quant Cytol 3: 285

    Google Scholar 

  60. Schenck U, Schwarz G (1983) Probleme der Präparationstechnik. Microsc Acta (Suppl 6 ): 13

    CAS  Google Scholar 

  61. Schwarz, G (1983) Ein Konzept für die routinemäßige Herstellung von automatengerechten gynäkologischen Präparaten. Microsc Acta (Suppl 6 ): 29

    CAS  Google Scholar 

  62. Schwarz G, Schwarz M (1983) Ermittlung notwendiger Zellzahlen zur sicheren Diagnostik bei gynäkologischen Suspensionspräparaten und mögliche Konsequenzen für die automatisierte Präparateanalyse. Microsc Acta (Suppl 6 ): 21

    CAS  Google Scholar 

  63. Schwarz, G, Schwarz M, Schenck U (1983) Effect of the special properties of monolayer cell preparations for automated cervical cytology on visual evaluation and classification: With an estimation of the number of cells required tobe screened. Analyt Quant Cytol 5: 189

    Google Scholar 

  64. Schwarz G (1984) Präparationstechnik für die automatische Zytologie. In: Automation der zytologischen Diagnostik. Deutsche Forschungs-u. Versuchsanstalt für Luft-u. Raumfahrt e.V. (DFVLR) (Hrsg) (1984) Köln: TÜV Rheinland 1984:55; Reihe: Ergebnisberichte zum Programm “Forschung und Entwicklung im Dienste der Gesundheit“, DFVLR NT - 1 84/3

    Google Scholar 

  65. Schwarz G (1985) A new centrifugal preparation method in clinical cytology and effects on the morphologic and cytochemical features of the cells. Acta Histochem (Suppl)

    Google Scholar 

  66. Schwarz G (1985) Automatisierung in der Zytologie - eine Begriffserklärung. Cyto-Info (VDCA) 5 (4): 19

    Google Scholar 

  67. Schwarz G, Roch J (1985) Zytomorphologische Heterogenität der Intermediärzellenpopulationen bei Exfoliativmaterial der Cervix uteri. Verh Dtsch Ges Path 69: 624

    Google Scholar 

  68. Schwarz G (1986) Die Arbeitsweise von vollautomatisch und interaktiv arbeitenden Zytoanalyse-Systemen. Cyto-Info (VDCA) 6 (2): 48

    Google Scholar 

  69. Soost HJ, Baur S (1980) Gynäkologische Zytodiagnostik: Lehrbuch und Atlas. 4. völlig neu bearbeitete Auflage. Georg Thieme Verlag, Stuttgart New York

    Google Scholar 

  70. Soost HJ, Brem W, Höbel W, Otto K, Schwarz G (1983) Comparison of Papanicolaou staining and Feulgen staining for automated prescreening Analyt Quant Cytol 5: 61

    CAS  Google Scholar 

  71. Soost HJ (1983) Automatisierte Zytodiagnostik aus der Sicht des klinischen Zytologen. Microsc Acta (Suppl 6 ): 169

    CAS  Google Scholar 

  72. Tanaka N, Ikeda H, Ueno T, Okamoto Y, Hosoi S (1981) CYBEST-CDMS: automated cell dispersion and monolayer smearing device for CYBEST. Analyt Quant Cytol 3: 96

    CAS  Google Scholar 

  73. Tanke HJ, van Ingen EM, Ploem JS (1979) Acriflavine-Feulgen-stilbene staining: A procedure for automated cervical cytology with a television based system ( LEYTAS ). J Histochem Cytochem 27: 84

    Google Scholar 

  74. Wenzelides K (1984) Hat die Präparationstechnik Einfluß auf die automatische Mikroskopbildanalyse? Bild und Ton 37: 56

    Google Scholar 

  75. Wied GL, Bartels PH, Bibbo M (1983) Die Begriffswelten der visuellen und automatischen Zytodiagnostik - wie eng berühren sie sich? Microsc Acta (Suppl 6 ): 179

    CAS  Google Scholar 

  76. Wittekind D, Reinhardt ER, Kretschmer V, Zipfel E (1983) Influence of staining on fast automated cell segmentation, feature extraction and cell image analysis. Analyt Quant Cytol 5: 55

    CAS  Google Scholar 

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© 1988 Springer-Verlag Berlin Heidelberg

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Reitan, J.B., Reith, A., Schwarz, G. (1988). Präparier- und Färbetechniken. In: Burger, G., Oberholzer, M., Gössner, W. (eds) Morphometrie in der Zyto- und Histopathologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73764-0_6

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  • DOI: https://doi.org/10.1007/978-3-642-73764-0_6

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