Skip to main content
SpringerLink
Log in
Book cover

Praxis der elektrophoretischen Trennmethoden pp 223–278Cite as

Praxis der präparativen Free-Flow-EIektrophorese

  • Chapter

Part of the book series: Anleitungen für die chemische Laboratoriumspraxis ((LABORATORIUM,volume 23))

Zusammenfassung

Das Prinzip der Free-Flow-Elektrophorese wird erstmals von Hannig und Grassmann [1-4] beschrieben. Während es sich bei den etablierten physikalisch-chemischen Trennverfahren zur Reinigung und Isolierung von Biopolymeren meist um diskontinuierliche Prozesse handelt, deren Trennkapazität durch die jeweils einsetzbare Probenmenge begrenzt ist, stellt die Free-Flow-Elektrophorese ein kontinuierliches Verfahren dar, bei dem die Elektrolyt- und Probenlösung die Trennanordnung senkrecht zu einem angelegten elektrischen Feld durchströmen. Je nach Trennprinzip lassen sich dabei folgende Verfahren unterscheiden:

  • Zonenelektrophorese

  • Isotachophorese

  • Isoelektrische Fokussierung

  • Feldsprungelektrophorese Somit steht mit der Free-Flow-Elektrophorese eine vielseitige Arbeitstechnik zur Verfügung, deren Anwendungsbereich die Trennung von anorganischen und organischen Ionen, von Peptiden, Proteinen, Enzymen bis hin zur Trennung von Zellen, subzellulären Partikeln, Viren sowie Bakterien umfaßt. Ein großer Vorteil der Free-Flow-Elektrophorese beruht auf dem Verzicht von Trägermaterialien zur antikonvektiven Stabilisierung des Trennsystems. Dadurch werden alle störenden Wechselwirkungen der Probe mit einem Träger vermieden. Die Anforderungen an die Apparaturen und an die Arbeitstechniken sind jedoch relativ hoch.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   59.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   79.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. Grassmann W, Hannig K, DBP 805 399, 24. 05. 1949

    Google Scholar 

  2. Grassmann W (1950) Über ein Verfahren zur Stofftrennung durch Kataphorese, Angew Chem 62, 170

    Google Scholar 

  3. Grassmann W, Hannig K (1950) Ein einfaches Verfahren zur kontinuierlichen Trennung von Stoffgemischen auf Filterpapier durch Elektrophorese. Naturwissenschaften 37, 397

    CAS  Google Scholar 

  4. Grassmann W (1951) Neue Verfahren der Elektrophorese auf dem Eiweißgebiet. Naturwissenschaften 38, 200

    CAS  Google Scholar 

  5. Barrolier J, Watzke E, Gibian H (1958) Einfache Apparatur für die trägerfreie kontinuierliche Durchlauf-Elektrophorese. Naturforsch 13B, 754

    Google Scholar 

  6. Hannig K (1961) Die trägerfreie kontinuierliche Elektrophorese und ihre Anwendung. Z Anal Chem 181, 244

    CAS  Google Scholar 

  7. Hannig K (1964) Eine Neuentwicklung der trägerfreien kontinuierlichen Elektrophorese. Hoppe Seyler’s Z Physiol Chem 338, 211

    CAS  Google Scholar 

  8. Hannig K, Wirth H, Meyer B, Zeiller K (1975) Free-flow Electrophoresis I. Theoretical and experimental investigations. Hoppe Seyler’s Z Physiol Chem 356, 1209

    CAS  Google Scholar 

  9. Kolin A (1967) Preparative electrophoresis in liquid columns stabilizised by electromagnetic rotation II. Artefacts, stability and resolution. J Chromatog 26, 180

    CAS  Google Scholar 

  10. Strickler A, Sachs T (1973) Continuous free-flow electrophoresis Crescent phenomen. Prep Biochem 3, 769

    CAS  Google Scholar 

  11. Hannig K, Heidrich H G, Die trägerfreie Ablenkungselektrophorese; in: “Handbuch der Elektrophorese”. Verlag Chemie, Weinheim, im Druck

    Google Scholar 

  12. Barth F, Gruetter M, Kessler R, Manz HJ (1986) The use of free-flow electrophoresis in the purification of recombinant human tissue plasminogen activator expressed in yeast. Electrophoresis 7, 372

    CAS  Google Scholar 

  13. Kessler R, Manz HJ, Walliser HP (1986) Purification of r-DNA products with free-flow electrophoresis. Thrd. Eng. Found. Conf. on Recovery of Bioproducts, Uppsala, Schweden

    Google Scholar 

  14. Kohlrausch F (1897) Über Konzentrationsverschiebungen durch Elektrolyse im Innern von Lösungen und Lösungsmittelgemischen. Ann Phys 62, 209

    Google Scholar 

  15. Kendall J, Crittenden FD (1923) Separation of isotopes. Proc Nat Acad Sci 9, 75

    CAS  Google Scholar 

  16. Acta Isotachophoretica 1976-1979, Reference List, LKB, Bromma, Schweden (1980)

    Google Scholar 

  17. Instrument Manual, LKB 2127 Tachophor, LKB, Bromma, Schweden (1977)

    Google Scholar 

  18. Kiso Y, Hirokawa T (1979) Correlation between formula weights and absolute mobilities obtained by isotachophoresis for alkylammonium and carboxylate ions. Chem Lets 891

    Google Scholar 

  19. Pospichal J, Demi M (1985) Determination of relative ionic mobilities by capillary isotachophoresis. J Chromatog 320, 139

    CAS  Google Scholar 

  20. Appl. Data No. 1, Capillary Type Isotachophoretic Analyzer, Shimadzu, Tokyo Japan

    Google Scholar 

  21. Kolin A (1954) Electrophoresis in density gradients combined with pH and/or conductivity gradients. J Chem Phys 22, 1628

    CAS  Google Scholar 

  22. Wagner H, Speer W (1978) Kontinuierliche trägerfreie Isoelektrische Fokussierung. J Chromatog 157, 259

    CAS  Google Scholar 

  23. Svensson H (1961) Isoelectric fractionation, analysis and characterization of ampholytes in natural pH-gradients I. The differential equation of solute concentrations at a steady state and its solution for simple cases. Acta Chem Scand 15, 325

    CAS  Google Scholar 

  24. Svensson H (1962) Isoelectric fractionation, analysis and characterization of ampholytes in natural pH-gradients II. The buffering capacity and conductance of isoionic ampholytes. Acta Chem Scand 16, 456

    CAS  Google Scholar 

  25. Svensson H (1962) Description of apparatus for electrolysis in columns stabilized by density gradients and direct determination of isoionic points. Arch Biochem Biophys Suppl 1, 132

    CAS  Google Scholar 

  26. Vesterberg O, Svensson H (1966) Isoelectric fractionation, analysis and characterization of ampholytes in natural pH-gradients IV. Further studies on the resolving power in connection with separation of myoglobins. Acta Chem Scand 20, 820

    CAS  Google Scholar 

  27. Binion S, Rodkey IS (1981) Simplified method for synthesizing ampholytes suitable for use in isoelectric focusing of immunoglobulins in agarose gel. Anal. Biochem 112, 232

    Google Scholar 

  28. Good NE, Winget GD (1966) Hydrogen ion buffers for biological research. Biochem 5, 467

    CAS  Google Scholar 

  29. Wagner H, Neupert D (1978) Kontinuierliche elektrophoretische Ionenfokussierung zur Trennung und Anreicherung von Metallionen. J Chromatog 147, 281

    CAS  Google Scholar 

  30. Wagner H, Neupert D (1978) Kontinuierliche elektrophoretische Ionenfokussierung II. Trennung und Anreicherung von Metallionen. J Chromatog 156, 219

    CAS  Google Scholar 

  31. Wagner H, Blatt H (1981) Kontinuierliche elektrophoretische Ionenfokussierung. Fresenius Z Anal Chem 306, 208

    CAS  Google Scholar 

  32. Wagner H, Kessler R (1983) Preparative free-flow protein focusing at the boundaries between solutions of different conductivity: Field step focusing; in “Analytical and Preparative Isotachophoresis”, Hrsg. Holloway CJ. Elsevier Scient Publ Comp Amsterdam

    Google Scholar 

  33. Wagner H, Heydt A, Paul H, Anwendung der Feldsprungelektrophorese zur Konzentrierung und Reinigung von Pflanzenviren. J Virol Meth, im Druck

    Google Scholar 

  34. Philpot JL (1983) in: “Methodological Developments in Biochemistry”. Hrsg. Reid E. Longmans England

    Google Scholar 

  35. Biostream Appl. Notes, CJB Developments Limited, England

    Google Scholar 

  36. Dickerson CH (1980) Developments of biological standardization; in: “3rd gen. Meeting of ESACT”, Hrsg. Karger S, Basel

    Google Scholar 

  37. Bier M, Egen NB, Allgyer TT, Twitty GE, Mosher RA (1979) in: “Peptides: Structure and Biological Function”, Hrsg. Gross E, Meienhofer J. Eds Pierce Chem. Co., Rockford, Illinois

    Google Scholar 

  38. Mosher RA, Egen NB, Bier M: Recycling instrumentation for preparative scale electrophoresis; in: “Protein purification: Micro to Macro”, Ucla Symp. on molecular and cellular biology, New Series 68, Hrsg. Burgess RLiss AR, New York (1987)

    Google Scholar 

  39. Binion SB, Rodkey LS, Egen NB, Bier M (1982) Rapid purification of antibodies to single band purity using recycling IEF. Electrophoresis 3, 284

    CAS  Google Scholar 

  40. Hannig K, Comsa J (1963) Fractionnement electrophoretic de l’extrait de thymus. Compt Rend Acad Sci 256, 1855

    CAS  Google Scholar 

  41. Hannig K (1963) Neue Anwendungen der trägerfreien kontinuierlichen Elektrophorese. Angew Chem 75, 1129

    Google Scholar 

  42. Hannig K, Wrba H (1964) Isolierung von vitalen Tumorzellen durch trägerfreie Elektrophorese. Naturforsch 19B, 860

    Google Scholar 

  43. Braunitzer G, Hobom G, Hannig K (1964) Zur Einheitlichkeit der Proteinstruktur. Z Physiol Chem 338, 276

    CAS  Google Scholar 

  44. Braunitzer G, Hobom G, Hannig K (1964) Ein einfaches Verfahren zur Selektionierung von Mutanten, Z Physiol Chem 338, 278

    CAS  Google Scholar 

  45. Mehl E, Jatzkewitz H (1964) Eine Cerebrosidsulfatase aus Schweineniere. Z Physiol Chem 339, 260

    CAS  Google Scholar 

  46. Guest JR, Yanovsky C (1965):Mutationally induced amino acid substitutions in a tryptic peptide of the tryptophan synthetase A protein. J Biol Chem 240, 679

    CAS  Google Scholar 

  47. Schlieselfeld LH, van Eys J, Touster O (1964) The purification and properties of a nucleotide pyrophosphatase of rat liver nuclei. J Biol Chem 240, 811

    Google Scholar 

  48. Rubin AL, Stenzel KH (1965) In vitro synthesis of brain protein. Proc Nat Acad Sci 53, 963

    CAS  Google Scholar 

  49. Rubin AL, Drake MP, Davison PF, Pfahl D, Speakman PT, Schmitt FO (1965) Effects of pepsin treatment on the interaction properties of tropocollagen macromolecules. Biochem 4, 181

    Google Scholar 

  50. Jaffe WG, Hannig K (1965) Fractionation of proteins from kidney beans. Arch Biochem Biophys 103, 80

    Google Scholar 

  51. Mashburn jr. TA, Hoffman P (1966) Preparative continuous-flow electrophoresis of acidic mucopolysaccharides. Anal. Biochem 16, 267

    CAS  Google Scholar 

  52. Chan SK, Tuloss I, Margollash E (1966) Primary structure of the cytochrom c from the snapping turtle (Chelydra Serpentina). Biochem 5, 2586

    CAS  Google Scholar 

  53. Bidwell E, Dike GWR, Denson KWE (1966) Experiments with factor VIII separated from fibrinogen by electrophoresis in free buffer film. Brit J Haematol 12, 583

    CAS  Google Scholar 

  54. Sarkar S (1966) Preparation of native tobacco mosaic virus protein by continuous free-flow electrophoresis. Naturforsch 2IB, 1202

    Google Scholar 

  55. Hannig K (1967) Preparative electrophoresis; in: “Electrophoresis”. Hrsg. Bier M, Acad Press, London

    Google Scholar 

  56. Klofat W, Hannig K (1967) Elektrophoretische Trennung von Chloroplastenfragmenten mit unterschiedlichem Verhältnis von Chlorophyll a: Chlorophyll b. Z Physiol Chem 348, 1332

    CAS  Google Scholar 

  57. Schweiger A, Hannig K (1967) Anwendung der trägerfreien kontinuierlichen Elektrophorese zur Reinigung von Rattenleber-Ribosomen. Z Physiol Chem 348, 1005

    CAS  Google Scholar 

  58. Francois CJ, Gilmcher MJ (1967) The isolation and amino acid composition of the alpha-chains of chicken-bone collagen. Biochim Biophys Acta 133, 91

    CAS  Google Scholar 

  59. Francois CJ, Gilmcher MJ (1967) The separation of the alpha-chains of collagen by free-flow electrophoresis. Biochem J 102–148

    Google Scholar 

  60. Nordwig AA, Dehm P, Jahn WF (1967) Carrier-free electrophoresis as a useful tool for the purification of mammalian and mold enzymes. Protides Biol Fluids Proc Colloq 15, 531

    Google Scholar 

  61. Sayre FW, Lee RT, Sandman RP, Perez-Mendez G (1967) Studies of a growth factor. Fractionation studies and amino acid composition of derived fractions. Arch Biochem Biophys 118, 58

    CAS  Google Scholar 

  62. Fröhlich C (1967) Arbeitserfahrungen mit einem trägerfreien kontinuierlichen Elektrophoreseverfahren und seine Anwendung auf Bindungsuntersuchungen. Klin Wochsch 45, 461

    Google Scholar 

  63. Schultz J, Feiberg N, John S (1967) Myeloperoxidase VIII. Separation into ten components by free-flow electrophoresis. Biochem Biophys Res Commun 28, 543

    CAS  Google Scholar 

  64. Jennings AC, Watt WB (1967) Fractionation of plant material I. Extraction of proteins and nucleic acids from plant tissues and isolation of protein fractions containing hydroxypyroline from broad bean (Vicia faba L.) leaves. J Sci Food Agri 18, 527

    CAS  Google Scholar 

  65. Klofat W, Hannig K (1967) Elektrophoretische Isolierung von Chloroplasten. Z Physiol Chem 348, 739

    CAS  Google Scholar 

  66. Heidrich HG, Hannig K (1968) The native form of beet liver catalase. Biochim Biophys Acta 168, 380

    CAS  Google Scholar 

  67. Hannig K, Krüssmann WF (1968) Die Anwendung der trägerfreien kontinuierlichen Elektrophorese zur Auftrennung der weißen Blutzellen aus Humanblut. Z Physiol Chem 349, 161

    CAS  Google Scholar 

  68. Schweiger A, Hannig K (1968) The electrophoretic isolation of protein associated with mRNA in rat liver nuclei. Z Physiol Chem 349, 943

    CAS  Google Scholar 

  69. Hannig K (1968) Die Ablenkungselektrophorese; Jahrbuch der Max-Planck-Gesellschaft, S. 117

    Google Scholar 

  70. Kern H (1968) Reindarstellung von Desoxyribonukleinsäure durch Elektrophorese. J Chromatog 32, 790

    CAS  Google Scholar 

  71. Nordwig A, Jahn WF (1968) A collagenolytic enzyme from Aspergillus oryzae. European J Biochem 3, 519

    CAS  Google Scholar 

  72. The application of free-flow electrophoresis to the separation of macromolecules and particles of biological importance; in: “Modern Separation Methods of Macromolecules and Particles”. Hrsg. Gerritson T, J. Wiley & Sons, Ins, Vol. 2 (1968)

    Google Scholar 

  73. Hannig K, Zeiller K (1969) Zur Auftrennung und Charakterisierung immunkompetenter Zellen mit Hilfe der trägerfreien Ablenkungselektrophorese. Z Physiol Chem 350, 748

    Google Scholar 

  74. Evans JJ (1969) Increased buffer density as a means of separating peroxidase isoenzymes by preparative continous-flow carrier-free electrophoresis. Anal Biochem 32, 101

    CAS  Google Scholar 

  75. Stahn R, Maier KP, Hannig K (1970) A new method for the preparation of rat liver lysosomes. J Cell Biol 46, 576

    CAS  Google Scholar 

  76. Heidrich HG, Hannig K (1970) Isolation of pure inner and outer mitochondrial membranes in preparative scale using a new technique; Advances in automatic analysis 347

    Google Scholar 

  77. Zeiller K, Pascher G, Hannig K (1970) The formation of 19S hemolysine producing cells in intestinal lymph nodes of the rat. Z Physiol Chem 351, 435

    CAS  Google Scholar 

  78. Heidrich HG, Stahn R, Hannig K (1970) The surface charge of rat liver mitochondria and of their membranes. Clarification of some controversis concerning the mitochondrial structure. J Cell Biol 46, 137

    CAS  Google Scholar 

  79. Schweiger A, Hannig K (1970) Proteins associated with rapidly labeled nuclear RNA and their occurence in rat liver cytoplasmic fractions. Biochim Biophys Acta 204, 317

    CAS  Google Scholar 

  80. de Groot K, Hoenders HJ, Leon A, Bloemendahl H (1970) Isolation of alpha-cristallin by means of continous-flow electrophoresis in a liquid film. Exp Eye Res 10, 71

    Google Scholar 

  81. Wöltgens JHM, Bonting SL, Bijvoet OLM (1970) Relationship of inorganic pyrophosphates and alkaline phosphatase activities in hamster. Molars Cals Tiss Res 5, 333

    Google Scholar 

  82. Blancot MT, Han K, Biserte G, Tacquet A (1970) Purification partielle et propriétés exo-peptidasique d’une fraction intracellulaire de mycobacterium phlei. Ann Inst Pasteur 119, 719

    Google Scholar 

  83. Alam SN, Alam RA, Harbers E (1970) Studies on deoxyribonucleoproteins VII. Purification of deoxyribonucleoproteins by carrier-free continuous electrophoresis. Biochim Biophys Acta 209, 550

    CAS  Google Scholar 

  84. Hannig K (1971) Free-flow electrophoresis; in: “Methods in Microbiology”. Hrsg. Norris JR, Ribbons DW, Acad. Press, London

    Google Scholar 

  85. Gailliard T (1971) The enzymic deacylation of phospholipids and galastolipids in plants; Biochem J 121, 279

    Google Scholar 

  86. Leon AE, Gerding JJT, de Groot K, Hoenders HJ, Bloemendal H (1971) The structure of the acidic polypeptide chains from alphacristallin. Amino acid composition, peptide mapping and N-terminus. Intern J Protein Res 3, 19

    CAS  Google Scholar 

  87. Robinson DS, Monsey JB (1971) Studies on the composition of eggwhite ovomucin. Biochem J 121, 537

    CAS  Google Scholar 

  88. Pusztal A, Watt WB (1971) Free-flow electrophoresis of proteins in phenol-containing solvents at various pH-values. Biochim Biophys Acta 251, 158

    Google Scholar 

  89. Nolan C, Margollasch E, Peterson JD, Steiner DF (1971) The structure of bovine proinsulin. J Biol Chem 246, 2780

    CAS  Google Scholar 

  90. Ryan KJ, Kalant H, Thomas E (1971) Free-flow electrophoretic separation and electrical surface properties of subcellular particles from guinea pig brain. J Cell Biol 49, 235

    CAS  Google Scholar 

  91. Zeiller K, Liebich HG, Hannig K (1971) Free-flow electrophoretic separation of lymphocytes. Two thoracid duct lymphozyte subpopulations studied after prolonged cannulation and SRBC immunication. European J Immunol 1, 350

    Google Scholar 

  92. Zeiller K, Hannig K (1971) Free-flow electrophoretic separation of lymphocytes. Evidence for specific organ distributions of lymphoid cells. Z Physiol Chem 352, 1162

    CAS  Google Scholar 

  93. Zeiller K, Hannig K, Pascher G (1971) Free-flow electrophoretic separation of lymphocytes.Separation of graft versus host reactive lymphocytes of rat spleens. Z Physiol Chem 352, 1168

    CAS  Google Scholar 

  94. Schweiger A, Hannig K (1971) Studies on the RNA-binding activity of rat liver cytosol. Purified RNA-binding factor of rat liver. Biochim Biophys Acta 254, 255

    CAS  Google Scholar 

  95. Thompson AR: Recent developments in separation methods. Proc Biochem Sept 1971, S. 35

    Google Scholar 

  96. Sotorres AM, Hörman H (1971) Entwicklung neuer Fischmehle in Argentinien II. Löslichkeit und Fraktionierung der Proteine. Arch Fischereiwiss. 22, 280

    CAS  Google Scholar 

  97. Zeiller K, Schubert JCF, Walther F, Hannig K (1972) Free-flow electrophoretic separation of bone marrow cells. Electrophoretic distribution analysis of in vivo colony forming cells in mouse bone marrow. Hoppe Seyler’s Z Physiol Chem 353, 95

    CAS  Google Scholar 

  98. Zeiller K, Holzberg E, Pascher G, Hannig K (1972) Free-flow electrophoretic separation of T and B lymphocytes. Evidence for various subpopulations of B cells. Hoppe Seyler’s Z Physiol Chem 353, 105

    CAS  Google Scholar 

  99. Heidrich HG, Kinne R, Kinne E, Saffran, Hannig K (1972) The poiarity of the proximal tubule cell in rat liver: Different surface charges for the brush-border microvilli and plasma-membranes from the basal infoldings. J Cell Biol 54, 232

    CAS  Google Scholar 

  100. Hannig K (1972) Fortschritte der Molekularbiologie als Resultat neuer Methoden; Mitteilungen der Max-Planck-Gesellschaft 2

    Google Scholar 

  101. Schubert JCF, Zeiller K, Walther F (1972) Zellelektrophoretische Trennung menschlicher Knoehenmarkszellen; in: “Leukämien und maligne Lymphome”, Hrsg. Stacher A, Urban und Schwarzenberg, München Berlin Wien

    Google Scholar 

  102. Schweiger A, Spitzauer P (1972) Identification and partial characterization of a rat liver cytosol-protein with high affinity to RNA and DNA. Biochim Biophys Acta 277, 403

    CAS  Google Scholar 

  103. Bitar KG, Vinogradov SN, Nolan C, Weiss LJ, Margollasch E (1972) The primary structure of cytochrome c from the rust fungus Ustilago sphaerogena. Biochem J 129, 561

    CAS  Google Scholar 

  104. Nordling S, Andersson LC, Häyry P (1972) Separation of T and B lymphocytes by preparative cell electrophoresis. European J Immunol 2, 405

    CAS  Google Scholar 

  105. Häyry P, Andersson LC, Nordling S, Virolainen M (1972) Allograft response in vitro. Transplant Rev 12, 92

    Google Scholar 

  106. Rübner H, Hövel H (1972) Einfluß der Ionenstärke und Osmolarität des Nährmediums auf die Membranladung von heteroploiden Kulturzellen. Zelltrennung mit Hilfe der trägerfreien kontinuierlichen Elektrophorese. Hoppe Seyler’s Z Physiol Chem 353, 1978

    Google Scholar 

  107. Schubert JC, Walther F, Holzberg E, Pascher G, Zeiller K (1973) Preparative electrophoretic Separation of normal and neoplastic human bone marrow cells. Klin Wochschr 51, 327

    CAS  Google Scholar 

  108. Zeiller K, Pascher G. (1973) Detection of T-and B-cells specific heteroantigens on electrophore-tically separated lymphocytes of the mouse. European J Immunol 3 614

    CAS  Google Scholar 

  109. Zucker R, Jauker U, Droege W (1973) Cellular composition of the chicken thymus. Effects of neonatal bursectomy and hydrocortisoné treatment. European J Immunol 3, 812

    CAS  Google Scholar 

  110. Fawcett JS (1973) Continuous isoelectric focusing and isotachophoresis. Ann N Y Acad Sci 200, 112

    Google Scholar 

  111. Andersson LC, Nordling S, Häyry P (1973) Fractionation of T and B lymphocytes by preparative cell electrophoresis. Cellular Immunol 8, 235

    CAS  Google Scholar 

  112. Heidrich HG, Kronschnabel O, Hannig K (1973) Eine Endopeptidase aus der Matrix von Rattenlebermitochondrien; Hoppe Seyler’s Z Physiol Chem 354, 1399

    CAS  Google Scholar 

  113. Stein G, Flad HD, Papst R, Trepel F (1973) Separation of human lymphocytes according to their surface charge; Meeting of the International Society of Experimental Hematology, Paris

    Google Scholar 

  114. Stein G, Flad HD, Papst R, Trepel F (1973) Separation of human lymphocytes by freeflow electrophoresis. Biomedicine 19, 388

    CAS  Google Scholar 

  115. Hamberg U, Stielwagen P, Ervast H-S (1973) Human macroglobulin characterization and trypsin binding, purification methods, trypsin and plasmin complex formations. European J Biochem 40, 439

    CAS  Google Scholar 

  116. Warnecke P, Kruse K, Harbers E (1973) Studies on deoxyribonucleoproteins VIII. Isolation and characterization of nonhistone proteins from euchromatic and heterochromatic deoxy-ribonucleoprotein of rat liver. Biochim Biophys Acta 331, 395

    Google Scholar 

  117. Droege W, Zucker R, Hannig K (1974) Developmental changes in the cellular composition of the chicken thymus. Cellular Immunology 12, 186

    CAS  Google Scholar 

  118. Blackburn TH, Hullah WA (1974) The cell-bound protease of bacteroides amylöphilus H 18. Can J Microbiol 20, 435

    CAS  Google Scholar 

  119. Heidrich HG, Leutner G (1974) Two types of vesicles from the erythrocytes ghost membrane differing in surface charge. Separation and characterization by preparative free-flow electro-phoresis. European J Biochem 41, 37

    CAS  Google Scholar 

  120. Pendyala I Wellman A (1974) The separation of certain bases, nucleosides and nucleotides related to adenine by continuous-flow electrophoresis. Anal Biochem 59, 634

    CAS  Google Scholar 

  121. von Boehmer H, Shortman K, Nossal GJV (1974) The separation of different cell classes from lymphoid organs X. Preparative electrophoretic separation of lymphoid subclasses from mousespleen and thoracic duct lymph. J Cell Physiol 83, 231

    Google Scholar 

  122. Reis JFG, Lightfood FN, Ho-Lun Lee (1974) Concentration profiles in free-flow electrophoresis. Aichi J 20, 362

    CAS  Google Scholar 

  123. von Boehmer H (1974) Separation of T and B lymphozytes and their role in the mixed lympho-zyte reaction. J Immunol 112, 70

    Google Scholar 

  124. Henning R, Heidrich HG (1974) Membrane lipids of rat liver lysosomes, prepared by free-flow electrophoresis. Biochim Biophys Acta 345, 326

    CAS  Google Scholar 

  125. Olsen WL, Heidrich HG, Hofschneider PH, Hannig K (1974) DNA-envelope complexes isolated from E. coli by free-flow electrophoresis. J Bacteriol 118, 646

    CAS  Google Scholar 

  126. Marchalonis JJ, Cone RE, von Boehmer H (1974) Surface immunoglobulines of peripheral thymus derived lymphocytes. Immuno-Chem 2, 271

    Google Scholar 

  127. Prusik Z (1974) Free-flow electromigration separations. J Chromatog 91, 867

    CAS  Google Scholar 

  128. Goldstone AH, Urbaniak SJ, Irvine WJ (1974) Clin Exp Immunol 17, 113

    CAS  Google Scholar 

  129. Albrecht SP, Heidrich HG (1975) Beef heart submitochondrial inner and outer membranes. Biochim Biophys Acta 376, 231

    Google Scholar 

  130. Dew ME, Heidrich HG (1975) The isolation of renin granules from rabbit kidney cortex by free-flow electrophoresis. Hoppe Seyler’s Z Physiol Chem 356, 621

    CAS  Google Scholar 

  131. Osmond DG, Miller RG, von Boehmer H (1975) Characterization of immunoglobulin-bearing and other small lymphocytes in mouse bone marrow by sedimentation and electrophoresis. J Immunol 114, 1230

    CAS  Google Scholar 

  132. Chant S, Silverman M (1975) Free-flow electrophoretic separation of renal tubular membranes from dog kidney. J Clinical Research 23, 650

    Google Scholar 

  133. Just WW, Leon-V JO, Werneer G (1975) Isoelectric focusing in continuous-flow electrophoresis I. Separation of mixed red blood cells or different species. Anal Biochem 67, 590

    CAS  Google Scholar 

  134. Colle A, Tonelle C, Leclerq M, Manuel Y (1975) The use of preparative continuous-flow electrophoresis to study low molecular weight proteins in urine. Clin Chim Acta 65, 223

    CAS  Google Scholar 

  135. Häyry P, Andersson LC, Gahmberg C, Roberts P, Rankl A, Nordling S (1975) Fractionation of immunocompetent cells by free-flow cell electrophoresis. Israel J Med Sci 11 (NI2), 1299

    Google Scholar 

  136. Schlegel RA, von Boehmer H, Shortman K (1975) Antigen-initiated B lymphocyte differentiation V. Electrophoretic separation of different subpopulations of AFC progenitors for unprimed IgM and memory IgG responses to the NIP determinant. Cell Immunol 16, 203

    CAS  Google Scholar 

  137. Harbers E, Hollandt H, Ellsers-König C, Schneider R, Struttmann C, Warnecke P (1975) Studies on deoxyribonucleoprotein IX. Subfractions of deoxyribonucleoproteins from rat and mouse liver. Hoppe Seyler’s Z Physiol Chem 356, 671

    CAS  Google Scholar 

  138. Melcher E, von Boehmer H, Phillips RA (1975) B lymphocyte subpopulations in the mouse. Organ distribution and ontogeny of immunoglobuline-synthezising and of mitogen-sensitive cells. Transpl Rev 25, 26

    Google Scholar 

  139. Shortman K, von Boehmer H, Lipp J, Hopper K (1975) Subpopulations of T lymphocytes, physical separation, functional specialization and differentiation, pathways of subsets of thymo-cytes and thymus-dependent peripheral lymphocytes. Transpl Rev 25, 163

    CAS  Google Scholar 

  140. Stein G (1975) Separation of human lymphoid cells by preparative cell electrophoresis. Z Immunol Exp 150, 68

    CAS  Google Scholar 

  141. Zeiller K, Löser R, Pascher G, Hannig K (1975) Free-flow electrophoresis II. Analysis of the method with respect to preparative cell separation. Hoppe Seyler’s Z Physiol Chem 356, 1226

    Google Scholar 

  142. Schwartz IL, Scott WN, Kinne R, Wyssbrod H, Roth LB, Walther R (1975) Membrane effects of neurophyseal hormones and related studies on parathyroid hormone; in: “Peptides: Chemistry, Structure and Biology”, Proceedings of the 4th American Peptide Symposium, Hrsg. Walter R, Meienhofer J, Ann Arbor Science Publ Inc, Ann Arbor, Michigan

    Google Scholar 

  143. Kinne R, Shlatz LJ, Kinne-Saffran E, Schwartz IL (1975) Distribution of membrane-bound cyclic AMP-dependent protein kinase in plasma membranes of cells of the kidney cortex. J Membrane Biol 24, 145

    CAS  Google Scholar 

  144. Heidrich HG, Olsen WL (1975) DNA complexes from E. coli. J Cell Biol 67, 444

    CAS  Google Scholar 

  145. Schubert JCF Walther F (1975) Adv Biosci 14, 299

    Google Scholar 

  146. Wigzell H (1975) International Union Cancer Tech Rep Sev 20, 140

    Google Scholar 

  147. Zeiller K, Schindler R, Liebich HG (1975) The T lymphocyte surface in development. Israel J Med Sci 11, 1242

    CAS  Google Scholar 

  148. Blumenstock F, Saba TM, Weber P, Cho E (1976) Purification and biochemical characterization of a macophage stimulating alpha-2-globulin opsonic protein. J Reticuloendothelal Soc 19, 157

    CAS  Google Scholar 

  149. Stein G (1976) Separation of human lymphoid cells by preparative cell electrophoresis III. Studies on cells of lymphoid organs in hematological normal persons. Biomedicine 24, 106

    CAS  Google Scholar 

  150. Stein G (1976) Separation of human lymphoid cells by preparative cell electrophoresis II. Free-flow electrophoretic separation of human blood cells. Biomedicine 23, 5

    Google Scholar 

  151. Dreker L, Schwarz J, Reichel W, Hilschmann N (1976) Die Primärstruktur eines monoklonalen Igl-Immunglobulins I. Reinigung und Charakterisierung des Proteins, der L-und H-Ketten, der Bromcyanspaltprodukte und der Disulfidbrücken. Hoppe Seyler’s Z Physiol Chem 357, 1515

    CAS  Google Scholar 

  152. Walther F, Schubert JCF, Schopow K (1976) Hämatol Bluttransfus 19, 47

    CAS  Google Scholar 

  153. Schopow K, Walther F, Schubert JCF (1976) European J Clin Invest 6, 312

    Google Scholar 

  154. Heidrich HG, Dew M (1976) Current problems in clinical biochemistry VI. Renal metabolism in relation to renal function, Hrsg. Schmidt U, Dubach UC. Hans Huber, Bern

    Google Scholar 

  155. Brunner G (1976) Separation of plasma-membranebound enzymes of thymocytes by free-flow electrophoresis. Hoppe Seyler’s Z Physiol Chem 367, 253

    Google Scholar 

  156. Just W (1976) Continuous-flow isoelectric focusing of the light mitochondrial fraction of rat. liver. Hoppe Seyler’s Z Physiol Chem 357, 266

    CAS  Google Scholar 

  157. Sachs G, Chang H, Rabon E, Schackmann R, Lewin M, Saccomani G (1976) A non-electrogenic H+-pump in plasma membranes of hog stomach. J Biol Chem 251, 7690

    CAS  Google Scholar 

  158. Zeiller K, Pascher G, Hannig K (1976) B lymphocyte subpopulations in the mouse spleen. A study of the differentiation pathway using free-flow electrophoretically separated subpopula-tions of direct PFC progenitor cells. Immunology 31, 863

    CAS  Google Scholar 

  159. Shortman K, Fidler JM, Schlegel RA, Nossal GJV, Howard M, Lipp J, von Boehmer H (1976) Subpopulations of B lymphocytes. Physical separation of functionally distinct stages of B cell differentiation: Contemporary topics in Immunology 5, 1

    CAS  Google Scholar 

  160. Bode F, Baumann K, Kinne R (1976) Analysis of the pinocytic process in rat kidney II. Biochemical composition of pinocytic vesicles compared to brush border microvilli lysosomes and basalateral plasma membranes. Biochim Biophys Acta 433, 294

    CAS  Google Scholar 

  161. Heidrich HG, Dew M (1976) Preparative free-flow electrophoresis for isolation of membrane, organelle and cell fractions from rabbit kidney cortex. Current Problems in Clinical Biochemistry 6, 108

    CAS  Google Scholar 

  162. Jenssen HL, Eckert R, Werner H, Kohler H, Pasternak G, Friemel H (1977) Oncology 34, 159

    CAS  Google Scholar 

  163. Dumont F, Robert F (1977) Immunology 32, 989

    CAS  Google Scholar 

  164. Dumont F (1977) Physical properties of mouse peripheral lymph node cells changes during development. Annales d’Immunologie 128, 1053

    CAS  Google Scholar 

  165. Blumenstock F, Weber P, Saba T (1977) Isolation and biochemical characterization of 2-opsonic glycoprotein from rat serum. J Biol Chem 252, 7156

    CAS  Google Scholar 

  166. Crockett FT (1977) An evaluation of free-flow electrophoresis as a means of separation of human palatine tonsil cells. Alabama J Med Sci 14, 435

    CAS  Google Scholar 

  167. Baudner S, Schweiger A, Günther HO (1977) Nachweiß von Sojaeiweiß aus auf 120 °C erhitzten Fleischkonserven. Mitt Gebiete Lebensm Hyg 68, 183

    CAS  Google Scholar 

  168. Coculescu M, Zooral M, Matulevicus V (1977) Tentative identification of arginineVasotocin in the bovine pineal extract prepared by the milcu-nanu method. Revue romaine de medicine, Ser Endocrinologie 15, 27

    CAS  Google Scholar 

  169. Schally AV, Redding TW, Arimura A, Dupont A, Linthicum GL (1977) Isolation of gamma-amino butyric acid from pig hypothalami and demonstration of its prolactin release-inhibiting (PIF) activity in vivo and in vitro. Endocronology 100, 681

    CAS  Google Scholar 

  170. Chang H, Saccomani G, Rabon E, Schackmann R, Sachs G (1977) H+-transport by gastric membrane vesicles. Biochim Biophys Acta 464, 313

    CAS  Google Scholar 

  171. Sachs G, Spenney JG, Rehm WS (1977) Gastric secretion; in: “Gastrointestinal physiology, II. Intl. Rev. Physiol 12”, Hrsg. Grane RK, University Park Press, Baltimore

    Google Scholar 

  172. Saccomani G, Steward HB, Shaw D, Levin M, Sachs G (1977) Characterization of gastric mucosal membranes IX. Fractionation and purification of K-ATPase containing vesicles by zonal centri-fugation and free-flow electrophoresis. Biochim Biophys Acta 465, 311

    CAS  Google Scholar 

  173. Suter DAI, Ferber E, Brunner G (1977) A comparison, after separation by free-flow electrophoresis of thymocyte plasma membrane vesicles prepared using different methods of cell disruption. Hoppe Seyler’s Z Physiol Chem 358, 313

    Google Scholar 

  174. Heidrich HG, Dew M (1977) Homogenous cell populations from rabbit kidney cortex. J Cell Biol 74, 780

    CAS  Google Scholar 

  175. Brunner G (1977) Surface charge properties of plasma membranes after enzymic treatment. Hoppe Seyler’s Z Physiol Chem 358, 221

    Google Scholar 

  176. Bol S, van Vliet M, van Slingerland V (1981) Electrophoretic mobility properties of murine hemopoeitic cells in different stages of development. Exp. Hematol 9, 431

    CAS  Google Scholar 

  177. Levy EM, Cooperband SR, Bennett M, Schmid K (1977) Separation of natural killer and mitogen induced cellular cytotoxicity effector cells by preparative free-flow electrophoresis. Federation proceedings 36, 1325

    Google Scholar 

  178. Sato S, Maeda M, Sando N (1977) Electrophoretic mobility of haploid cells, diploid cells, asci and ascospores of saccharomyces cerevisiae. Plant and Cell Physiol 18, 931

    Google Scholar 

  179. Motomiya T, Yomazaki H, Sawasaki Y, Nakajima H (1977) The relationship among plateled age, volume and electrophoretic mobility. Thrombosis and Haemastasis 38, 1

    Google Scholar 

  180. Schubert JCF, Schopov K, Walther F (1977) Die elektrophoretische Beweglichkeit der Blut-lymphozyten bei chronischer lymphatischer Leukämie. Blut 35, 135

    CAS  Google Scholar 

  181. Spiegel K, Lutz R (1977) Meßdatenerfassung bei der Elektrophorese. Elektronik 5, 67

    Google Scholar 

  182. Levy EM, Yonkosky D, Schmid K, Cooperband SR (1977) Enrichment of the natural killer and mitogen induced cellular cytotoxicity cells using preparative free-flow high voltage electrophoresis. Preparative Biochemistry 7, 467

    CAS  Google Scholar 

  183. Sachs G, Rabon E, Chang H, Schackmann R, Sarau HM, Saccomani G (1977) A H +-pump and fusion model in stomach; in: “Hormonal receptors in digestive tract physiology”. Hrsg. Bonfils S, Gromageot P, Rosselin G. Elsevier Publ. Co., Amsterdam

    Google Scholar 

  184. Kreisberg JI, Sachs G, Pretlow IG, McGuire RA (1977) Separation of proximal tubule cells by free-flow electroophoresis. J Cell Biol 93, 169

    CAS  Google Scholar 

  185. Kinne R, Schwartz IL (1977) The asymmetrical distribution of renal epithelial cell membrane function in the action of antidiuretic hormone and parathyroid hormone; in: “Disturbances in body fluid osmolarity”, Hrsg. Andeoli TE, Grantham JJ, Rector jr. FC, American Physiological Society

    Google Scholar 

  186. Kinne R (1977) Die Funktion der Nierenmembranen. Naturwissenschaften 64, 324

    Google Scholar 

  187. Brunner G, Heidrich HG, Golechi JR, Bauer HC, Stuter D, Plückhahn D, Ferber E (1977) Fractionation of membrane vesicles II. Method for separation of membrane vesicles bearing different enzymes by free-flow electrophoresis. Biochim Biophys Acta 471, 195

    CAS  Google Scholar 

  188. Chant S, Silverman M (1977) Immunologic characterization of plasma membranes from the renal proximal tubule of the dog. Kidney International 2, 348

    Google Scholar 

  189. Kirk G, Prusiner S (1977) Comparative studies on membranes from bovine choroid plexus and rat kidney cortex. Life Science 21, 833

    CAS  Google Scholar 

  190. Ostrach S (1977) Convection in continuous-flow electrophoresis. J Chromatog 140, 187

    CAS  Google Scholar 

  191. Sherbet GV (1978) The biophysical characterization of the cell surface. Academic Press, New York

    Google Scholar 

  192. Charlier G, Leimen J. Wellemans G, Strobbe R (1978) A Studie of the electrophoretic mobilities of the viruses of swine fever and bovine viral diarrhoea. Veterinary Science Comm 2, 105

    Google Scholar 

  193. Ellis S, Vodian MA, Grindeland RE (1978) Studies on the proassayable growth hormone-like activity of plasma. Recent progress in Hormone Research 34, 213

    CAS  Google Scholar 

  194. Levy EM, Silverman S, Schmid K, Cooperband SR (1978) Mitogen induced cellular cytotoxic activity as a monocycle function in human peripheral blood preparations. Cellular Immunity 40, 222

    CAS  Google Scholar 

  195. Blumenstock FA, Saba TM, Weber P (1978) Purification of alpha-2-opsonic protein from human serum and its measurement by immunoassay. J Reticuloendothelial Soc 23, 119

    CAS  Google Scholar 

  196. Yaoral M, Flegel M, Barth T, Machova A (1978) Purification of tengrammes amounts of (1)-Mercaptopropionic acid, 8-D-Arginine Vasopressin on a continuous free-flow electrophoresis. Chemical and pharmacological data of DDAVP and BY-products. Collection Czechoslov Chem Commun 43, 511

    Google Scholar 

  197. Zeiller K, Pascher G (1978) Differentiation antigens in rat lymphocyte subpopulations recognized by rabbit antisera against electrophoretically separated cells. European J Immunol 8, 469

    CAS  Google Scholar 

  198. Heidrich HG, Albracht PJ, Bäckström D (1978) Two iron-sulfur centers in mitochondrial outer membranes from beef hearts as prepared by free-flow electrophoresis. FEBS letters 95, 314

    CAS  Google Scholar 

  199. Dubacq J-P, Kader JC (1978) Free-flow electrophoresis of chloroplasts. Plant Physiol 61, 465

    CAS  Google Scholar 

  200. Chollet P, Herve P, Chassagne J, Masse M, Plagne R, Peters A (1978) Application of cell electrophoretic methods (preparative and analytical) in demonstrating the surface heterogenity of T lymphocytes in man. Biomedicine 28, 119

    CAS  Google Scholar 

  201. Giannovario JA, Griffin R, Gray EL (1978) A mathematical model of free-flow electrophoresis. J Chromatog 153, 329

    CAS  Google Scholar 

  202. Kuntz G, Stöckel P, Heidrich HG (1978) The conformer nature of the multiple forms of beef liver catalase as obtained by biomedical and small-angle X-ray scattering experiments. A model for the quarternary structure of the beef liver catalase molecule. Hoppe Seyler’s Z Physiol Chem 359, 959

    CAS  Google Scholar 

  203. Heidrich HG, Rüssmann L, Bayer B, Jung A (1979) Free-flow electrophoresis for the separation of malaria infected and uninfected mouse erythrocytes and for the isolation of free parasites (Plasmodium vinckei). A new rapid technique for the liberation of malaria parasites from their host cells. Z Parasitenkunde 58, 151

    CAS  Google Scholar 

  204. Kolin A, Ellerbroek BL (1979) Theory of simultanous nultiple streak collimation in continuous flow electrophoresis by superposition of electroosmosis and thermal convection. Separation and Purification Methods 8, 1

    CAS  Google Scholar 

  205. Puzas JE, Vignery A, Rasmussen H (1979) Isolation of specific bone cell types by free-flow electrophoresis. Calsif Tiss Intl 27, 263

    CAS  Google Scholar 

  206. Suzuki M, Sawasaki Y, Wahl S, Iwaoka H, Asada T, Nakajima H (1979) Separation of plasmodiumberghei-parasitized rat erythrocytes by means of carrier-free electrophoresis. Bull World Health Organization 57, 129

    CAS  Google Scholar 

  207. Pretlow TG, Pretlow TP (1979) Cell electrophoresis. International Review of Cytology 61, 85

    CAS  Google Scholar 

  208. Lavrov VF, Shestakova SV, Sitkovskii MV, Gulubeva NN (1979) Electrophoretic separation of lymphocytes from rat spleen and thymus and their response to mitogens in vitro. Bull Exp Biol Med 88, 1035

    Google Scholar 

  209. Vandewalle A, Heidrich HG (1979) Characterization of different populations of rabbit kidney cortical cells separated by free-flow electrophoresis using hormonestimulated adenylate cyclases. Intern J Biochem 10, IX Uppsala J Med Sci Suppl 26 no 74

    Google Scholar 

  210. Voet L, Hannig K, Zeiller K (1979) Cytofluorometric analysis of R-Thy-1 antigens in various rat lymphocytes with different electrophoretic mobility and organ distributions. J Histochem Cytochem 27, 426

    CAS  Google Scholar 

  211. Pretlow TP, Steward HB, Sachs G, Pretlow TG, Pitts A (1979) Proc Am Assoc Cancer Res 20, 124

    Google Scholar 

  212. Sturk A, Burt L, Heidrich HG, Crawford N (1979) Subfractionation of platelet granules by high voltage continuous electrophoresis in a free flowing buffer film. Thromb Haemost 42, 214

    Google Scholar 

  213. Ramsey WS, Nowlan ED, Simpson LB (1980) Resolution of microbial mixtures by free-flow electrophoresis. European J Appl Microbiol Biotechnol 9, 217

    Google Scholar 

  214. Harms E, Kern H, Schneider JA (1980) Human lysosomes can be purified from diploid skin fibroplasts by free-flow electrophoresis. Proc Nat Acad Sci 77, 6139

    CAS  Google Scholar 

  215. Rustin P, Kader MJ (1980) Isolement par electrophorese en veine liquide de mitochondries de feuilles d’epinard. Compt Rend 291, 105

    CAS  Google Scholar 

  216. Ivory CF (1980) Continuous-flow electrophoresis. The crescent phenomena revisted I. Isothermal effects. J Chromatog 195, 165

    CAS  Google Scholar 

  217. Vandewalle A, Heidrich HG (1980) Characterization of different populations of rabbit kidney cortical cells separated by free-flow electrophoresis using hormone stimulated adenylate cyclase. Intern J Biochem 12, 61

    CAS  Google Scholar 

  218. Heidrich HG, Geiger R (1980) Kininogenase activity in plasmamembranes and cell organells from rabbit kidney cortex-subcellularlocalization of renal kallikrein by free-flow electrophoresis and density gradient fractionation. Kideney Intern 18, 77

    CAS  Google Scholar 

  219. Koubek K, Kristofova H, Rieger M, Hilfert I (1980) Partial purification and separation of H-2 antigens by preparative free-flow electrophoresis. Folia Biologica 26, 123

    CAS  Google Scholar 

  220. McGuire J, Miller TY, Tipps RW, Snyder RS, Righetti PR (1980) New experimental approaches to the isoelectric fractionation of cells. J Chromatog 194, 323

    CAS  Google Scholar 

  221. Jilg W, Hannig K, Zeiller R (1980) Radioionidated surface proteins of electrophoretically separated rat lymphocytes. Hoppe Seyler’s Z Physiol Chem 361, 389

    CAS  Google Scholar 

  222. Basset P, Froissart C, Vincendon G, Massarelli DR (1980) A continuous-flow isoelectric focusing system for protein fractionation, its description and application with partial purification of cholinacetyl-transpherase. Electrophoresis 1, 168

    CAS  Google Scholar 

  223. Heidrich HG, Dansforth H, Beaudoin R (1980) Free-flow electrophoretic separation of whole body plasmodium berghei sporozoites. European J Cell Biol 22, 516

    Google Scholar 

  224. Heidrich HG, Rieckmann KH (1980) Free-flow electrophoresis separation of intracellular parasites (Plasmodium falciparum). European J Cell Biol 22, 516

    Google Scholar 

  225. Sheeler P, Doolittle MH (1980) Intern. Laboratory 3, 71

    Google Scholar 

  226. Menashi S, Weintroub H, Crawford N (1980) Separation of platelet plasma membranes by high voltage electrophoresis. Proc. of the 13th Federation of the European Biochemical Societies Meeting, Jerusalem

    Google Scholar 

  227. Menashi S, Crawford N (1980) Isolation of platelet surface and intracellular membranes by high voltage free-flow electrophoresis. European J Cell Biol 22, 598

    Google Scholar 

  228. Dumont F, Reichert E (1979) Identification of feline blood B and T lymphocytes by cell electrophoresis. Comparative Immunol Microbiol and infections deseases 2, 23

    CAS  Google Scholar 

  229. Wagner H, Mang V (1981) Apparatus and application of preparative free-flow isotachophoresis; in: “Analytical Isotachophoresis”. Hrsg. Everaets FM, Elsevier Scient Publ, Amsterdam

    Google Scholar 

  230. Menashi S, Weintroub H, Crawford N (1981) Characterization of human platelet surface and intracellular membranes isolated by free-flow electrophoresis. J Biol Chem 256, 4095

    CAS  Google Scholar 

  231. Ivory CF (1981) Continuous-flow electrophoresis. The crescent phenomena revisted II. Non-isothermal effects. Electrophoresis 2, 31

    CAS  Google Scholar 

  232. Biber J, Stieger B, Haase W, Murer H (1981) Biochim Biophys Acta 647, 169

    CAS  Google Scholar 

  233. Bischoff P, Robert F, Donner M (1981) British J Cancer 44, 545

    CAS  Google Scholar 

  234. Pretlow TP, Steward HB, Sachs G, Pretlow TG, Pitts A (1981) British J Cancer 43, 537

    CAS  Google Scholar 

  235. Heidrich HG (1981) Free-flow electrophoresis in malaria research; in: “Electrophoresis’ 81”. Hrsg. Allen RC, Arnaud P, Walther de Gruyter, Berlin

    Google Scholar 

  236. Sabolovic N, Sabolovic D, Guilmin AM (1977) T and B cell surface markers on rabbit lymphocytes. Immunology 32, 581

    CAS  Google Scholar 

  237. Ilsbroux I, Vanduffel L, de Cuyper M, Joniau M, Teuchy H (1981) Partial purification of Ca2+-ATPase from pig kidney cortex by free-flow electrophoresis. Biochem Soc Trans 9, 136

    Google Scholar 

  238. Boltz jr. RC, Schmatz DM, Murray PK (1981) Evaluation of the electrophoretic separability of trypanosoma cruzi parasites stages; in: “Electrophoresis’ 81”. Hrsg. Allen RC, Arnaud P, Walther de Gruyter, Berlin

    Google Scholar 

  239. Harms E, Kartenbeck J, Darai G, Schneider J (1981) Purification and characterization of human lysosomes from EB-virus transformed lymphoblasts. Experimental Cell Research 131, 251

    CAS  Google Scholar 

  240. Harms E, Gocham N, Schneider J (1981) Lysosomal pool of free amino acids. Biochem Biophys Res Commun 99, 830

    CAS  Google Scholar 

  241. Miller AL, Kress BC, Stein R, Kinnon C, Kern H, Schneider JA, Harms E (1981) Properties of N-acetyl-β-D-hexosaminidase from isolated normal and I-cell lysosomes. J Biol Chem 256, 9352

    CAS  Google Scholar 

  242. Vandewalle A, Köpfer-Hobelsberger B, Heidrich HG (1982) J Cell Biol 92, 505

    CAS  Google Scholar 

  243. Hansen E (1982) Blut 44, 141

    CAS  Google Scholar 

  244. Hansen E, Hannig K (1982) Antigen-specific electrophoretic cell separation (ASECS). Isolation of human T and B lymphocyte subpopulations by free-flow electrophoresis after reaction with antibodies. J Immunol Methods 51, 197

    CAS  Google Scholar 

  245. Heidrich HG, Mrema JEK, Jagd DLV, Reyes P, Rieckmann KH (1982) Isolation of intracellular parasites (Plasmodium falsiparum) from culture using free-flow electrophoresis. Separation of free parasites according to stages. J Parasitol 68, 443

    CAS  Google Scholar 

  246. Hannig K (1982) New aspects in preparative and analytical free-flow cell electrophoresis. Electrophoresis 3, 235

    CAS  Google Scholar 

  247. de Cuyper M, Joniau M, Dangreau H (1982) Mechanism of phospholipid transfer between artificial vesicles. Arch Intern Physiol Biochimie 90b, 15

    Google Scholar 

  248. Murray PK, Boltz RC, Schmatz DM (1982) Separation of individual stages of trypanosoma cruzi grown in cell culture by continuous free-flow electrophoresis. J Protozool 29, 109

    CAS  Google Scholar 

  249. Kempner D, Smolka A, Rembaum A (1982) Electrophoretic cell separation using polyacrolein microspheres. Electrophoresis 3, 109

    Google Scholar 

  250. Smolka A, Kempner D, Renbaum A (1982) Electrophoretic separation of human lymphocytes by means of immunmicrospheres. Electrophoresis 3, 300

    CAS  Google Scholar 

  251. Heidrich HG: Cell population from the renal proximal distal tubule isolated by free-flow electrophoresis; in: “Cell function and differentiation, Teil I”, Hrsg. Allan R, Liss Inc. New York

    Google Scholar 

  252. Wagner H, Blatt H, Lamberty MA (1982) Trennung und Anreicherung von Metallkationen durch kontinuierliche elektrophoretische Ionenfokussierung; in: “Atomspektrometrische Spurenanalytik”, Hrsg. Weltz B, Verlag Chemie, Weinheim

    Google Scholar 

  253. Baudner S, Schweiger A, Günther O (1982) Reinigung löslicher Maisproteine nach dem Prinzip der trägerfreien Elektrophorese und Nachweis der Proteine durch immunologische Verfahren in Bier. Lebensmitteluntersuchung und-forschung 175, 17

    CAS  Google Scholar 

  254. Wagner H, Kessler R: Free-flow field-step focusing — a new method for preparative protein focusing; in: “Electrophoresis’ 82”, Hrsg. Stathakos D, Walther de Gruyter, Berlin New York

    Google Scholar 

  255. Wagner H, Kessler R (1983) Preparative free-flow protein focusing at the boundaries between solutions of different conductivity: Fieldstep focusing; in: “Analytical and Preparative Iso-tachophoresis”, Hrsg. Holloway CJ, Elsevier Scient Publ, Amsterdam

    Google Scholar 

  256. Wagner H, Mang V, Kessler R, Manzoni R (1983) A free-flow system for preparative isotacho-phoresis; in: “Analytical and Preparative Isotachophoresis”. Hrsg. Holloway CJ, Elsevier Scient Publ, Amsterdam

    Google Scholar 

  257. Wagner H, Mang V (1983) Preparative free-flow isotachophoresis; in: “Analytical and Preparative Isotachophoresis”. Hrsg. Holloway CJ, Elsevier Scient Publ, Amsterdam

    Google Scholar 

  258. de Cuyper M, Joniau M, Dangreau H (1983) Intervesicular phospholipid transfer. A free-flow electrophoresis study. Biochemistry 22, 415

    Google Scholar 

  259. Wagner H, Mang V, Kessler R, Heydt A, Manzoni R (1984) Preparative free-flow electrophoresis of proteins, peptides and related compounds; in: “Electrophoresis’ 83”. Hrsg. Hirai H, Walther de Gruyter, Berlin

    Google Scholar 

  260. Morre DJ, Morre DM, Heidrich HG (1983) Subfractionation of the rat liver golgi apparatus by free-flow electrophoresis. European J Cell Biol 31, 263

    CAS  Google Scholar 

  261. Heidrich HG, Dansforth HD, Leef JL, Beaudoin RL (1983) Free-flow electrophoresis separation of pasmodium berghei sporozoites. J Parasitol 69, 360

    CAS  Google Scholar 

  262. Seller FR, Johannsen R, Sedlacek HH, Zeiller K (1974) Characterization of lymphocyte sub-populations of nonhuman primates separated by free-flow electrophoresis. Transpl Proc 6, 173

    Google Scholar 

  263. Baier TG, Weber G, Heinrich U, Hartmann K, Schönberg HD (1988) Analytical Biochemistry 171, 91–95

    CAS  Google Scholar 

Download references

Authors
  1. H. Wagner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. Kuhn
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Hoffstetter
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Fachrichtung Anorganische Analytik und Radiochemie, Universität des Saarlandes, 6000, Saarbrücken, Germany

    Horst Wagner

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag Berlin, Heidelberg

About this chapter

Cite this chapter

Wagner, H., Kuhn, R., Hoffstetter, S. (1989). Praxis der präparativen Free-Flow-EIektrophorese. In: Wagner, H., Blasius, E. (eds) Praxis der elektrophoretischen Trennmethoden. Anleitungen für die chemische Laboratoriumspraxis, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73622-3_6

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-73622-3_6

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73623-0

  • Online ISBN: 978-3-642-73622-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation