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Praxis der zwei-dimensionalen Elektrophorese von Proteinen

  • Chapter
Praxis der elektrophoretischen Trennmethoden

Part of the book series: Anleitungen für die chemische Laboratoriumspraxis ((LABORATORIUM,volume 23))

Zusammenfassung

Die Methode wurde unter verschiedenen Namen bekannt: High Resolution Two-Dimensional Electrophoresis, Protein Mapping, Protein Subunit Mapping, ISO-DALT System. Im Laufe der Zeit hat diese Methode jedoch eine so starke Verbreitung gefunden, so daß sich für diese Technik der allgemeine Name„ zwei-dimensionale Elektrophorese“ (2DE oder 2D) durchgesetzt hat, obwohl es sich hier um eine spezielle Form der zweidimensionalen Elektrophorese handelt. Im Folgenden wird nur auf diese spezielle Technik eingegangen. Sie wird mit der Abkürzung 2DE bezeichnet.

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Literatur

  1. Macko V, Stegemann H (1969) Hoppe-Seyler’s Z Physiol Chem 350, 917–919

    CAS  Google Scholar 

  2. Dale G, Latner AL (1969) Clin Chim Acta 24, 61–68

    Article  CAS  Google Scholar 

  3. Kenrick KG, Margolis J (1970) Anal Biochem 33, 204–207

    Article  CAS  Google Scholar 

  4. Raymond S (1964) Annals N.Y. Acad Sci 121, 350–365

    Article  CAS  Google Scholar 

  5. Margolis J, Kenrick KG (1969) Nature 221, 1056–1057

    Article  CAS  Google Scholar 

  6. Kaltschmidt E, Wittmann HG (1970) Anal Biochem 36, 401–412

    Article  CAS  Google Scholar 

  7. Barrett T, Gould HJ (1973) Biochim Biophys Acta 294, 165–170

    CAS  Google Scholar 

  8. MacGillivray AJ, Rickwood D (1974) Eur J Biochem 41, 181–190

    Article  CAS  Google Scholar 

  9. Bhakdi S, Knüfermann H, Hoelz/Wallach DF (1974) Biochim Biophys Acta 345, 448–457

    Article  CAS  Google Scholar 

  10. Stegemann H, Francksen H, Macko V (1973). Z F Naturforsch 28. 722–732

    Google Scholar 

  11. Wrighley C (1970) Biochem Gentics 4, 509–516

    Article  Google Scholar 

  12. Klose J (1975) Humangenetik 26, 231–243

    CAS  Google Scholar 

  13. Klose J (1975) In: New Approaches to the Evaluation of Abnormal Embryonic Development, Eds. Neubert D, Merker HJ, Thieme G Stuttgart, 375–387

    Google Scholar 

  14. Klose J, von Wallenberg-Pachaly H (1976) Dev Biol 51, 324–331

    Article  CAS  Google Scholar 

  15. O’Farrell PH (1975) J Biol Chem 250, 4007–4021

    Google Scholar 

  16. Scheele GA (1975) J Biol Chem 250, 5375–5385

    CAS  Google Scholar 

  17. Iborra F, Buhler JM (1976) Anal Biochem 74, 503–511

    Article  CAS  Google Scholar 

  18. Klose J, Blohm J, Gerner I (1977) In: Methods in Prenatal Toxicology. Eds. Neubert D, Merker H-J, Kwasigroch TE, Thieme G, Stuttgart, 303–313

    Google Scholar 

  19. Klose J (1979) Genetics 92 (suppl.), 13–24

    Google Scholar 

  20. Klose J, Feller M (1981) Electrophoresis 2, 12–24

    Article  CAS  Google Scholar 

  21. Klose J (1983) In: Modern Methods in Protein Chemistry, Ed. Tschesche H. Walter de Gruyter Berlin, New York, in press

    Google Scholar 

  22. Anderson NG, Anderson NL (1978) Anal Biochem 85, 331–340

    Article  CAS  Google Scholar 

  23. Anderson NL, Anderson NG (1978) Anal Biochem 85, 341–354

    Article  CAS  Google Scholar 

  24. Garrels JI (1979) J Biol Chem 254, 7961–7977

    CAS  Google Scholar 

  25. Voris BP, Young DA (1980) Anal Biochem 104, 478–484

    Article  CAS  Google Scholar 

  26. Manabe T, Tachi K, Kojima K, Okuyama T (1979) J Biochem 85 649–659

    CAS  Google Scholar 

  27. Poehling HM, Neuhoff V (1980) Electrophoresis 1, 90–102

    Article  CAS  Google Scholar 

  28. Sinclair J, Rickwood D (1981) In: Gel Electrophoresis of Proteins: A Practical Approach, Eds. Hames BD, Rickwood D, IRL Press Ltd., London, 189–218

    Google Scholar 

  29. Sidmann C (1981) In: Immunological Methods, Vol. II, Eds. Lefkovits I, Pernis B, Academic Press, New York, 57–74

    Google Scholar 

  30. Ames GFL, Nikaido K (1976) Biochemistry 15, 616–623

    Article  CAS  Google Scholar 

  31. Goldsmith MR, Rattner EC, Koehler MD, Balikov SR, Bock SC (1979) Anal Biochem 99, 33–40

    Article  CAS  Google Scholar 

  32. Görg A, Postel W, Westermeier R, Gianazza E, Righetti PC (1980) J Biochem Biophys Methods 3, 273–284

    Article  Google Scholar 

  33. Bjellqvist B, Ek K, Righetti PG, Gianazza E, Görg A, Westermeier R, Postel W (1982) J Biochem Biophys Methods 6, 317–339

    Article  CAS  Google Scholar 

  34. Tracy RP, Currie RM, Young DS (1982) Clin Chem 28, 908–914

    CAS  Google Scholar 

  35. Ornstein L (1964) Annals NY Acad Sci 121, 321–349

    Article  CAS  Google Scholar 

  36. Davis BJ (1964) Annals N Y Acad Sci 121, 404–427

    Article  CAS  Google Scholar 

  37. Klose J, Nowak J, Kade W (1980) In: Electrophoresis’ 79, Ed. Radola BJ, Walter de Gruyter, Berlin, 297–312

    Google Scholar 

  38. Bonner WM, Laskey RA (1974) Eur J Biochem 46, 83–88

    Article  CAS  Google Scholar 

  39. Laskey RA, Mills AD (1975) Eur J Biochem 56, 335–341

    Article  CAS  Google Scholar 

  40. Gruenstein EI, Pollard AL (1976) Anal Biochem 76, 452–457

    Article  CAS  Google Scholar 

  41. Walton KE, Styer D, Gruenstein EI (1979) J Biol Chem 254, 7951–7960

    CAS  Google Scholar 

  42. McConkey EH (1979) Anal Biochem 96, 39–44

    Article  CAS  Google Scholar 

  43. Choo KH, Cotton RGH, Danks DM (1980) Anal Biochem 103, 33–38

    Article  CAS  Google Scholar 

  44. Lecocqu R, Hepburn A, Lamy F (1982) Anal Biochem, in press

    Google Scholar 

  45. Kuhn O, Wilt FH (1980) Anal Biochem 105, 274–280

    Article  CAS  Google Scholar 

  46. Finger JM, Choo KH (1981) Biochem J 193, 371–374

    CAS  Google Scholar 

  47. Wilson DL, Hall ME, Stone GC, Rubin RW (1977) Anal Biochem 83, 33–44

    Article  CAS  Google Scholar 

  48. Horst MN, Mahaboob S, Basha M, Baumbach GA, Mansfield EH, Roberts RM (1980) Anal. Biochem. 102, 309–408

    Article  Google Scholar 

  49. Willard KE, Giometti C, Anderson NL, O’Connor TW, Anderson NG (1979) Anal Biochem 100, 289–298

    Article  CAS  Google Scholar 

  50. Sanders MM, Groppi VE, Browning ET (1980) Anal Biochem 103, 157–165

    Article  CAS  Google Scholar 

  51. Pulleyblank DE, Booth GM (1981) J Biochem Biophys Methods 4, 339–346

    Article  CAS  Google Scholar 

  52. O’Farrell PZ, Goodman HM, O’Farrell PH (1977) Cell 12, 1133–1142

    Article  Google Scholar 

  53. Altland K, Rossmann U (1985) Electrophoresis 6, 314–325

    Article  CAS  Google Scholar 

  54. Gianazza E, Frigerio A, Tagliabue A, Righetti PG (1984) Electrophoresis 5, 209–216

    Article  CAS  Google Scholar 

  55. Görg A, Postel W, Günther S, Weser J (1985) Electrophoresis 6, 599–604 Siehe no

    Article  Google Scholar 

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Authors
  1. J. Klose
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  2. M. Schmid
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Editor information

Editors and Affiliations

  1. Fachrichtung Anorganische Analytik und Radiochemie, Universität des Saarlandes, 6000, Saarbrücken, Germany

    Horst Wagner

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© 1989 Springer-Verlag Berlin, Heidelberg

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Klose, J., Schmid, M. (1989). Praxis der zwei-dimensionalen Elektrophorese von Proteinen. In: Wagner, H., Blasius, E. (eds) Praxis der elektrophoretischen Trennmethoden. Anleitungen für die chemische Laboratoriumspraxis, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73622-3_5

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  • DOI: https://doi.org/10.1007/978-3-642-73622-3_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73623-0

  • Online ISBN: 978-3-642-73622-3

  • eBook Packages: Springer Book Archive

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