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Praxis der ultradünnschicht-isoelektrischen Fokussierung mit Trägerampholyten und immobilisierten pH-Gradienten für ein- und zwei-dimensionale Trennungen

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Praxis der elektrophoretischen Trennmethoden

Part of the book series: Anleitungen für die chemische Laboratoriumspraxis ((LABORATORIUM,volume 23))

Zusammenfassung

Die isoelektrische Fokussierung (IEF) ist ein Elektrophoreseverfahren in einem pH-Gradienten. Hierbei werden amphotere Makromoleküle wie z. B. Peptide, Proteine, Enzyme und Hormone nach ihren isoelektrischen Punkten (pI) aufgetrennt. Die Bestimmung der Ladungsheterogenität ist ein empfindlicher Nachweis zur Unterscheidung sehr ähnlicher Moleküle. Da die getrennten Fraktionen an ihren isoelektrischen Punkten konzentriert (fokussiert) werden und nicht der Diffusion unterliegen, besitzt die Elektrofokussierung ein äußerst hohes Auflösungsvermögen. Entsprechend zahlreich sind daher die Anwendungsgebiete [1, 2].

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Authors
  1. A. Görg
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  2. W. Postel
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  3. S. Günther
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  4. R. Westermeier
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Editors and Affiliations

  1. Fachrichtung Anorganische Analytik und Radiochemie, Universität des Saarlandes, 6000, Saarbrücken, Germany

    Horst Wagner

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Görg, A., Postel, W., Günther, S., Westermeier, R. (1989). Praxis der ultradünnschicht-isoelektrischen Fokussierung mit Trägerampholyten und immobilisierten pH-Gradienten für ein- und zwei-dimensionale Trennungen. In: Wagner, H., Blasius, E. (eds) Praxis der elektrophoretischen Trennmethoden. Anleitungen für die chemische Laboratoriumspraxis, vol 23. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73622-3_4

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