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Plant Protoplasts and Genetic Engineering I pp 147–159Cite as

Isolation and Culture of Citrus Protoplasts

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 8))

Abstract

Citrus fruits rank second only to grapes in world fruit production. Citrus production was estimated in 1985 at a total of approximately 60 million metric tons (FAO 1985). Oranges comprise the most important Citrus product in world trade, followed by grapefruit, mandarins (which have recently increased, especially on the European market) and lemons. In addition to fresh fruits, large quantities of sweet orange, grapefruit and mandarin are processed into juice, concentrates, squashes and other products.

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References

  • Barrett HC (1977) Intergeneric hybridization of Citrus and other genera in Citrus variety improvement. Proc Int Soc Citric 1977, 2:586–589

    Google Scholar 

  • Barrett HC, Rhodes AM (1976) A numerical taxonomic study of affinity relationships in cultivated Citrus and its close relatives. Syst Bot 1:105–136

    Article  Google Scholar 

  • Ben-Hayyim G, Neumann H (1983) Stimulatory effect of glycerol on growth and somatic embryogenesis in Citrus callus cultures. Z Pflanzenphysiol 110:331–337

    CAS  Google Scholar 

  • Burger DW, Hackett WP (1982) The isolation, culture and division of protoplasts from Citrus cotyledons. Physiol Plant 56:324–328

    Article  Google Scholar 

  • Button J (1978) The effect of some carbohydrates on the growth and organization of Citrus ovular callus. Z Pflanzenphysiol 88:61–68

    CAS  Google Scholar 

  • Button J, Kochba J (1977) Tissue culture in the Citrus industry (refs p 207–248). In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 70–92

    Google Scholar 

  • Cameron JW, Frost HB (1968) Genetic breeding and nucellar embryony, Vol II. In: Reuther W, Batchelor LD, Webber HJ (eds) The Citrus industry. University of California Press, Berkeley, pp 325–381

    Google Scholar 

  • Cooper C, Chapot H (1977) Fruit production with special emphasis on fruit for processing, Vol II. In: Nagy S, Show PE, Veldhuis MK (eds) Citrus science and technology. AVI Publ, Westport, pp 1–27

    Google Scholar 

  • FAO (1985) Production yearbook. FAO Rome 39:194–196

    Google Scholar 

  • Frost HB, Soost RK (1968) Seed production: development of gametes and embryos, Vol II. In: Reuther W, Batchelor LD, Webber HJ (eds) The Citrus industry. University of California Press, Berkeley, pp 292–334

    Google Scholar 

  • Galun E, Aviv D (1983) Cytoplasmic hybridization. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of Plant CeU Culture, Vol I. Macmillan, New York, pp 358–392

    Google Scholar 

  • Galun E, Aviv D (1986) Organelle transfer. Methods in Enzymology 118:595–611

    Article  CAS  Google Scholar 

  • Galun E, Aviv D, Raveh D, Vardi A, Zelcer A (1977) Protoplasts in studies of cell genetics and morphogenesis. In: Barz W, Reinhard E, Zenk MH (eds) Plant tissue culture and its bio-technological application. Springer, Berlin Heidelberg New York, pp 302–312

    Chapter  Google Scholar 

  • Gmitter FG, Moore GA (1986) Plant regeneration from undeveloped ovules and embryogenic calli of Citrus: Embryo production, germination and plant survival. Plant Cell Tissue Organ Cult 6:139–147

    Article  CAS  Google Scholar 

  • Green RM, Vardi A, Galun E (1986) The plastome of Citrus: Physical map, variation among Citrus cultivars and species and comparison with related genera. Theor Appl Genet 72:170–177

    Article  CAS  Google Scholar 

  • Hensz RA (1971) Star Ruby, a new deep-red-fleshed grapefruit variety with distinct tree characteristics. J Rio Grande Val Hortic Soc 25:54–58

    Google Scholar 

  • Hidaka T, Yamada Y, Shichijo T (1979) In vitro differentiation of haploid plants by anther culture in Poncirus trifoliata. Jpn J Breed 29:248–254

    Google Scholar 

  • Iwamasa M (1966) Study on sterility in genus Citrus with special reference to the seedlessness. Bull Hortic Res Stn Jpn (Ser B) 6:1–77

    Google Scholar 

  • Kobayashi S, Uchimiya H, Ikeda I (1983) Plant regeneration from Trovita orange protoplasts. Jpn J Breed 33:119–122

    Google Scholar 

  • Kobayashi S, Ikeda I, Nakatani M (1984) Induction of nucellar callus from orange (Citrus sinensis Osb.) ovules and uniformity of regenerated plants (Japanese with English abstract). Bull Fruit Tree Res Stn Ser E (AWtsu) 5:32–54

    Google Scholar 

  • Kobayashi S, Ikeda I, Uchimiya H (1985) Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts. Plant Cell Tissue Organ Cult 4:249–259

    Article  Google Scholar 

  • Kochba J, Spiegel-Roy P (1973) Effect of culture media on embryoid formation from ovular callus of Shamouti orange (Citrus sinensis). Z Pflanzenziicht 69:156–162

    Google Scholar 

  • Kochba J, Spiegel-Roy P, Saad S, Neumann H (1978) Stimulation of embryogenesis in Citrus tissue culture by galactose. Naturwissenschaften 65:261

    Article  CAS  Google Scholar 

  • Kochba J, Spiegel-Roy P, Safran H (1972) Adventive plants from ovules and nucelli in Citrus. Planta 106:237–245

    Article  Google Scholar 

  • Murashige T, Tucker DPH (1969) Growth factor requirement of Citrus tissue culture. In: Chapman HD (ed) Proc 1st Int Citrus Symp Vol 3, Riverside CA, pp 1155–1161

    Google Scholar 

  • Ochatt SJ, Caso OH (1986) Shoot regeneration from leaf mesophyll protoplasts of wild pear (Pyrus communis var. Pyraster L.). J Plant Physiol 122:243–249

    CAS  Google Scholar 

  • Ohgawara D, Kobayashi S, Ohgawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 71:1–4

    Article  Google Scholar 

  • Soost RK (1964) Self-incompatibility in Citrus grandis (Linn.) Osbeck. Proc Am Soc Hortic Sci 84:137–140

    Google Scholar 

  • Swingle WT, Reece PC (1967) The botany of Citrus and wild relatives, Vol I. In: Reuther W, Batchelor LD, Webber JH (eds) The Citrus Industry. University of California Press, Berkeley, pp 190–430

    Google Scholar 

  • Vardi A (1977) Isolation of protoplasts in Citrus. Proc Int Soc Citric 1977, 2:575–578

    Google Scholar 

  • Vardi A (1981) Protoplast-derived plants from different Citrus species and cultivars. Proc Int Soc Citric 1981, 1:149–152

    Google Scholar 

  • Vardi A, Raveh D (1976) Cross feeder experiments between tobacco and orange protoplasts. Z Pflanzenphysiol 78:350–359

    Google Scholar 

  • Vardi A, Spiegel-Roy P (1978) Citrus breeding, taxonomy and the species problem. Proc Int Soc Citric 1978, 1:51–57

    Google Scholar 

  • Vardi A, Spiegel-Roy P, Galun E (1975) Citrus cell culture: Isolation of protoplasts, plating densities, effect of mutagens and regeneration of embryos. Plant Sci Lett 4:231–236

    Article  Google Scholar 

  • Vardi A, Spiegel-Roy P, Galun E (1982) Plant regeneration from Citrus protoplasts: Variability in methodological requirements among cultivars and species. Theor Appl Genet 62:171–176

    Article  Google Scholar 

  • Vardi A, Hutchinson DJ, Galun E (1986) A protoplast-to-tree system in Microcitrus based on protoplasts derived from sustained embryogenic callus. Plant Cell Rep 5:412–414

    Article  Google Scholar 

  • Vardi A, Breiman A, Galun E (1987) Citrus cybrids: Production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75:51–58

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Agricultural Research Organization, The Volcani Center, Institute of Horticulture, Bet Dagan, 50250, Israel

    Aliza Vardi

  2. Department of Plant Genetics, The Weizmann Institute of Science, Rehovot, 76100, Israel

    Esra Galun

Authors
  1. Aliza Vardi
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  2. Esra Galun
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Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1989 Springer-Verlag Berlin Heidelberg

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Vardi, A., Galun, E. (1989). Isolation and Culture of Citrus Protoplasts. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_9

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  • DOI: https://doi.org/10.1007/978-3-642-73614-8_9

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73616-2

  • Online ISBN: 978-3-642-73614-8

  • eBook Packages: Springer Book Archive

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