Abstract
Potato, Solarium tuberosum, is one of the most important crops in the temperate climate zones. It occupies the fifth position among the cultivated plant species with respect to yield and the fourth position as measured by area of cultivation (Barocka and Ross 1985). The present annual production amounts to about 300 million t harvested on about 22 million ha (Geisler 1983). The cultivation is being increased, particularly in developing countries. It tolerates all climate zones, from temperate to tropic, and from humid to arid.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Austin S, Baer MA, Helgeson JP (1985a) Transfer of resistance to potato leaf roll virus from Solarium brevidens into Solarium tuberosum by somatic fusion. Plant Sci 39:75–82
Austin S, Baer MA, Ehlenfeldt M, Kazmierczak PJ, Helgeson JP (1985 b) Intra-specific fusions in Solarium tuberosum. Theor Appl Genet 71:172–175
Austin S, Ehlenfeld MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypie variation under field conditions. Theor Appl Genet 71:682–690
Bajaj YPS (1986) Cryopreservation of potato somaclones. In: Semal J (ed) Somaclonal variations and crop improvement. Martinus Nijhoff, Dordrecht, pp 244–250
Bajaj YPS (ed) (1987) Biotechnology in agriculture and forestry 3. Potato. Springer, Berlin Heidelberg New York Tokyo
Barker H, Harrison BD (1982) Infection of potato mesophyll protoplasts with five plant viruses. Plant Cell Rep 1:247–249
Barocka KH, Ross H (1985) Kartoffel. In: Hoffmann W, Mudra A, Plarre W (eds) Lehrbuch der Züchtung landwirtschaftlicher Kulturpflanzen; Band 2: Spezieller Teil. Paul Parey, Berlin, pp 212–287
Barsby TL, Shepard JF (1983) Regeneration of plants from mesophyll protoplasts of Solarium species of the Etuberosa group. Plant Sci Lett 31:101–105
Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Somatic hybridization in the genus Solarium: S. tuberosum and S. brevidens. Plant Cell Rep 3:165–167
Binding H (1966) Regeneration und Verschmelzung nackter Laubmoosprotoplasten. Z Pflanzen-physiol 55:305–321
Binding H (1974) Regeneration von haploiden und diploiden Pflanzen aus Protoplasten von Petunia hybrida L. Z. Pflanzenphysiol 74:327–356
Binding H (1985) Regeneration of plants. In: Fowke LC, Constabel F (eds) Plant protoplasts. CRC Press, Boca Raton, Florida, pp 21–37
Binding H, Kollmann R (1985) Regeneration of protoplasts. In: Schafer-Menuhr A (ed) In Vitro Techniques. Propagation and Long Term Storage, CEC, Nijhoff Junk, Dordrecht Boston Lancaster, pp 93–99
Binding H, Mordhorst G (1984) Haploid Solarium dulcamara L.: Shoot culture and plant regeneration from isolated protoplasts. Plant Sci Lett 35:77–79
Binding H, Nehls R (1977) Regeneration of isolated protoplasts to plants in Solarium dulcamara L. Z Pflanzenphysiol 85:279–280
Binding H, Nehls R (1982) Techniques of somatic cell hybridization by fusion of protoplasts. In: Shay JW (ed) Techniques in somatic cell genetics. Plenum Press, New York London, pp 471–492
Binding H, Nehls R, Schieder O, Sopory SK, Wenzel G (1978) Regeneration of mesophyll protoplasts isolated from dihaploid clones of Solarium tuberosum. Physiol Plant 43:52–54
Binding H, Nehls R, Kock R, Finger J, Mordhorst G (1981) Comparative studies on protoplast regeneration in herbaceous species of the Dicotyledoneae class. Z Pflanzenphysiol 101:119–130
Binding H, Jain SM, Finger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine resistant biotype of Solarium nigrum with Solarium tuberosum. Part I: clonal variation in morphology and atrazine sensitivity. Theor Appl Genet 63:273–277
Binding H, Gdrschen E, Jorgensen J, Krumbiegel-Schroeren G, Ling HQ, Mordhorst G, Witt D, Kollmann R (1986) Protoplast regeneration and fusion in agarose lenses. In: Somers DA, Gengen-bach BG, Biesboer DD, Hackett WP, Green CE (eds) VI Internat Congr of Plant Tissue and Cell Culture-Abstracts, Univ Minnesota, Minneapolis, p 318
Binding H, Witt D, Monzer J, Mordhorst G, Kollmann R (1987) Plant cell graft chimeras obtained by co-culture of isolated protoplasts. Protoplasma 141:64–73
Binding H, Görschen E, Jörgensen J, Krumbiegel-Schroeren G, Ling HQ, Rudnick J, Sauer A, Zuba M, Mordhorst D (1988a) Protoplast culture in agarose media with particular emphasis to streaky culture lenses. Botanica Acta 101:233–239
Binding H, Zuba M, Rudnick J, Mordhorst G (1988b) Protoplast gel fusion. J Plant Physiol, in press
Bokelmann GS, Roest S (1983) Plant regeneration from protoplasts of potato (Solarium tuberosum cv. Bintje). Z Pflanzenphysiol 109:259–263
Butenko RG, Kuchko AA (1980) Somatic hybridization of Solarium tuberosum L. and Solarium cha-coense Bitt. by protoplast fusion. In: Ferenczy L, Farkas GL (eds) Advances in protoplast research. Acad. Kiado, Budapest, pp 293–300
Butenko RG, Kuchko AA, Vitenko VA, Avetisov VA (1977) Production and cultivation of isolated protoplasts from mesophyll of leaves of Solarium tuberosum L. and Solarium chacoense Bitt. Sov Plant Physiol 24:541–546
Butenko RG, Chromova LM, Sednina GV, Kuchko AA, Burgutin AB, Komarnitzky IK (1984) New forms of potato created by cell and protoplast culture methods. In: Rohlich P, Bacsy E (eds) Tissue culture and RES. Akademiai Kiado, Budapest, pp 515–527
Carlberg I, Glimelius K, Eriksson T (1983) Improved culture ability of protoplasts by use of activated charcoal. Plant Cell Rep 2:223–225
Carlberg I, Glimelius K, Eriksson T (1984) Nuclear DNA content during the initiation of callus formation from isolated protoplasts of Solarium tuberosum L. Plant Sci Lett 35:225–230
Creissen GP (1984) Protoplast regeneration and selection for toxin resistance in Solarium. Ph. D. Thesis, University of London
Creissen GP, Karp A (1985) Karyotypic changes in potato plants regenerated from protoplasts. Plant Cell Tissue Organ Cult 4:171–182
Debnath SC, Wenzel G (1987) Selection of somatic fusion products in potato by hybrid vigour. Potato Res 30:371–380
Debnath SC, Schuehmann R, Wenzel G (1986) Regeneration capacity of potato protoplasts isolated from single cell derived donor plants. Acta Bot Neerl 35:233–241
De Vries SE, Bokelmann GS (1986) Regeneration of callus and plants from cell suspension protoplasts of dihaploid potato (Solarium tuberosum L.). J Plant Physiol 122:199–203
De Vries SE, Tempelaar MJ (1987) Electrofusion and analysis of potato somatic hybrids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry. 3. Potato. Springer, Berlin Heidelberg New York Tokyo, pp 211–222
De Vries SE, Van Everdink WJ, Ferwerda MA, Jacobsen E, Pijnacker LP, Feenstra WJ (1987) Studies on gene mapping in the potato using somatic hybrids of Solarium tuberosum+Nicotiana plum-baginifolia. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in protoplast research. Kluwer Acacl Publ, Dordrecht Boston London, pp 215–218
Ehlenfeldt MK, Helgeson JP (1987) Fertility of somatic hybrids from protoplast fusions of Solarium brevidens and S. tuberosum. Theor Appl Genet, 73:395–402
Fish N, Jones MGK (1987) Production and field assessment of somatic hybrids between dihaploid Solarium tuberosum and Solarium brevidens. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, p 201
Fish N, Karp A (1986) Improvements in regeneration from protoplasts of potato and studies on chromosome stability. 1. The effect of initial culture media. Theor Appl Genet 72:405–412
Fish N, Karp A, Jones MGK (1987) Improved isolation of dihaploid Solarium tuberosum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro Cell Dev Biol 23:575–580
Foroughi-Wehr B, Stolle K (1985) Resistenzselektion in vitro am Beispiel des Systems Kartof-fel/Phytophthora infestans (Mont.) de Bary. Nachrichtenbl Dtsch Pflanzenschutzdienst 37: 170–173
Foulger D, Jones MGK (1986) Improved efficiency of genotype-dependant regeneration from protoplasts of important potato cultivars. Plant Cell Rep 5:72–76
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
Geisler G (1983) Ertragsphysiologie von Kulturarten des gemäßigten Klimas. Paul Parey, Berlin
Gill BS, Kam-Morgan LNW, Shepard JF (1986) Origin of chromosomal and phenotypic variation in potato protoclones. J Hered 77:13–16
Gleddie S, Keller WA, Setterfield G (1985) Plant regeneration from tissue, cell and protoplast cultures of several wild Solanum species. J Plant Physiol 119:405–418
Gressel J, Cohen N, Binding H (1984) Somatic hybridization of an atrazine resistant biotype of Solanum nigrum with Solanum tuberosum. 2. Segregation of plastomes. Theor Appl Genet 67:131–134
Grun P, Chu LJ (1978) Development of plants from protoplasts of Solanum (Solanaceae) Am J Bot 65:538–543
Gunn RE, Shepard JF (1981) Regeneration of plants from mesophyll-derived protoplasts of British potato (Solanum tuberosum L.). Plant Sci Lett 22:97–101
Haberlach GT, Cohen BA, Reichert NA, Towill LE, Helgeson JP (1985) Isolation, culture and regeneration of protoplasts from potato and several related Solanum species. Plant Sci 39:67–74
Hassanpour-Estahbanati A, Demarly Y (1985) Plant regeneration from protoplasts of Solanum pennellii: Effect of photoperiod applied to donor plant. J Plant Physiol 121:171–174
Helgeson JP, Hunt GJ, Haberlach GT, Austin S (1986) Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance. Plant Cell Rep 3:212–214
Helgeson JP, Haberlach GT, Pohlman J, Austin S (1987) Somatic fusions of Solanum species. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 205–207
Jia J-F, Potrykus I (1981) Mesophyll protoplasts from Solanum melongena var. depressum Bailey regenerate to fertile plants. Plant Cell Rep 1:71–72
Jones MGK (1984) Protoplast and somaclonal variation research on potato. In: Innovative methods for propagating potatoes, CIP, Lima, pp 305–318
Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 15:355–367
Kao KN, Michayluk MR (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110
Karlsson SB, Eriksson T (1987) Somatic hybridization between anther-derived dihaploid lines of Solanum tuberosum L. (potato). In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 197–198
Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272
Keller WA, Melchers G (1973) The effect of high pH and calcium on tobacco leaf protoplast fusion. Z Naturforsch Sect C Biosci 28:737–741
Kemble RJ, Shepard JF (1984) Cytoplasmic DNA variation in a potato protoclonal population. Theor Appl Genet 69:211–216
Kemble RJ, Barsby TL, Wong RSC, Shepard JF (1986) Mitochondrial DNA rearrangements in somatic hybrids of Solarium tuberosum and Solarium brevidens. Theor Appl Genet 72:787–793
Kowalczyk TP, Mackenzie IA, Cocking EC (1983) Plant regeneration from organ explants and protoplasts of the medicinal plant Solarium khasianum CB Clarke var. Chatterjeeanum Sengupta (Syn. Solarium viarum Dunal). Z Pflanzenphysiol 111:55–68
Kuchko AA (1985) Interspecies somatic hybridization of wild and cultivated potatoes. In: Butenko RG (ed) Plant Cell Culture. MIR, Moscow, pp 154–174
Landsmann J, Uhrig H (1985) Somaclonal variation in Solarium tuberosum detected at the molecular level. Theor Appl Genet 71:500–505
Li GG, Constabel F (1984) Plant regeneration with callus and protoplasts of Solarium uporo Dun. J Plant Physiol 117:137–142
Masson J, Lecerf M, Rousselle P, Perennee P, Pelletier G (1987) Plant regeneration from protoplasts of diploid potato derived from crosses of Solarium tuberosum with wild Solanum species. Plant Sci 53:167–176
Matern U, Strobel G, Shepard J (1978) Reaction to phytotoxins in potato population derived from mesophyll protoplasts. Proc Natl Acad Sci USA 75:4935–4939
Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Cornmun 43:203–218
Möllers C, Deimling S, Wenzel G (1987) Fusion of dihaploid Solarium tuberosum protoplasts and trials of hybrid selection with phytotoxins. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koorn-neef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 199–200
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497
Nehls R (1978) Isolation and regeneration of protoplasts from Solarium nigrum L. Plant Sci Lett 12:183–187
Nehls R, Krummbiegel-Schroeren G, Binding H (1986) Development of protoplast fusion products. In: Reinert J, Binding H (eds) Results and problems in cell differentiation 12, Differentiation of protoplasts and of transformed plant cells. Springer, Berlin Heidelberg New York Tokyo, pp 67–108
Nelson RS, Creissen GP, Bright SWJ (1983) Plant regeneration from protoplasts of Solarium brevidens. Plant Sci Lett 30:355–362
Ninnemann H, Jüttner F (1981) Volatile substances from tissue cultures of potato, tomato and their somatic fusion products. Comparison of gas chromatographic patterns for identification of hybrids. Z Pflanzenphysiol 103:95–108
Okamura M (1987) Regeneration and evaluation of somatic hybrid plants between Solarium tuberosum and Lycopersicon pimpinellifolium. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koornneef A J, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 213–214
Peloquin SJ, Hougas RW, Gabert AC (1966) Haploidy as a new approach to the cytogenetics and breeding of Solarium tuberosum. In: Riley R, Lewis E J (eds) Chromosome manipulations and plant genetics. Oliver and Boyd, Edinburgh, pp 21–28
Puite KJ, Roest S, Pijnacker LP (1986) Somatic hybrid plants after electrofusion of diploid Solarium tuberosum and S. phureja. Plant Cell Rep 5:262–265
Puite KJ, Ten Broeke WRR, Schaart JG, Schel JHN (1987) Flow cytometric sorting of potato heterokaryons with inhibition of cell wall synthesis before sorting. In: Puite KJ, Dons JJM, Huizing H J, Kool A J, Koornneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 209–210
Saxena PK, Gill R, Rashid A, Maheshwari SC (1981) Plantlet formation from isolated protoplasts of Solarium melongena L. Protoplasma 106:355–359
Saxena PK, Gill R, Rashid A, Maheshwari SC (1982) Plantlets from mesophyll protoplasts of Solarium xanthocarpum. Plant Cell Rep 1:219–220
Schieder O (1982) Somatic hybridization: a new method for plant improvement. In: Vasil I, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press, New York, pp 239–253
Schilde-Rentschler L, Boos G, Ninnemann H (1987) Somatic hybridization of diploid potato lines, a tool in potato breeding. In: Puite KJ, Dons JJM, Huizing HJ, Kool AJ, Koomneef M, Krens FA (eds) Current plant science and biotechnology in agriculture, Progress in plant protoplast research. Kluwer Acad Publ, Dordrecht Boston London, pp 195–196
Schuchmann R (1985) In vitro Selektion auf Fusarium Resistenz bei der Kartoffel. Ph. D, Thesis, TU Munchen
Schumann U, Opatrny Z, Koblitz H (1980) Plant recovery from long term callus cultures and from suspension culture derived protoplasts of Solarium phureja. Biochem Physiol Pflanz (BBP) 175:670–675
Secor GA, Shepard JF (1981) Variability of protoplast-derived potato clones. Crop Sci 21:102–105
Shahin EA (1984) Isolation, culture and regeneration of potato leaf protoplasts from plants preconditioned in vitro. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants. Academic Press, Orlando, San Diego New York London Toronto Montreal Sydney Tokyo, pp 381–390
Sharzhynskaya MV, Cherep NN, Gleba Y (1982) Potato and tobacco hybrid cell lines and plants obtained by cloning individual protoplast fusion products. Sov Cytol Genet 6:42–48
Shepard JF (1980a) Abscissic acid-enhanced shoot initiation in protoplast-derived calli of potato. Plant Sci Lett 18:327–333
Shepard JF (1980b) Mutant selection and plant regeneration from potato mesophyll protoplasts. In: Rubenstein I, Gengenbach B, Phillips RL, Green CE (eds) Genetic improvement of crops: emergent techniques. Univ of Minnesota Press, Minneapolis, pp 185–219
Shepard JF (1982) Cultivar dependent cultural refinements in potato protoplast regeneration. Plant Sci Lett 26:127–132
Shepard JF, Totten RE (1977) Mesophyll cell protoplasts of potato: isolation, proliferation and plant regeneration. Plant Physiol 60:313–316
Sidorov VA, Kuchko AA, Gleba YY (1984) Genetic modification of Solarium tuberosum through protoplast culture and fusion. In: Röhlich P, Bácsy E (eds) Tissue Culture and Research Akád Kiadó, Budapest, pp 529–534
Smillie RM, Melchers G, v. Wettstein D (1979) Chilling resistance of somatic hybrids of tomato and potato. Carlsberg Res Commun 44:127–132
Sree Ramulu K, Dijkhuis P, Roest S (1983) Phenotypic variation and ploidy level of plants regenerated from protoplasts of tetraploid potato (Solarium tuberosum L. cv. Bintje). Theor Appl Genet 65:329–338
Sree Ramulu K, Dijkhuis P, Roest S, Bokelmann GS, de Groot B (1984a) Early occurrence of genetic instability in protoplast cultures of potato. Plant Sci Lett 36:79–86
Sree Ramulu K, Dijkhuis P, Roest S (1984b) Genetic instability in protoclones of potato (Solarium tuberosum L. cv. Bintje): new types of variation after vegetative propagation. Theor Appl Genet 68:515–519
Thomas E (1981) Plant regeneration from shoot-culture derived protoplasts of tetraploid potato (Solarium tuberosum cv. Maris Bard). Plant Sci Lett 23:84–88
Thomas E, Bright SWJ, Franklin J, Lancaster VA, Miflin BJ (1982) Variation amongst protoplast-derived potato plants (Solatium tuberosum vc. Maris Bard). Theor Appl Genet 62:65–68
Uhrig H (1981) Regeneration of protoplasts of dihaploid potato plants bleached by a herbicide (SAN 6706). Mol Gen Genet 181:403–405
Uijtewaal BA, Suurs LCJM, Jacobsen E (1987) Protoplast fusion of monohaploid (2n = × = 12) potato clones: identification of somatic hybrids using malate dehydrogenase as a biochemical marker. Plant Sci 51:277–284
Wallin A, Glimelius K, Eriksson T (1974) The induction of aggregation and fusion of Daucus carota protoplasts by polyethylenglycol. Z Pflanzenphysiol 74:64–80
Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single cell culture derived Solarium tuberosum L. plants and a model for their application in breeding programmes. Theor Appl Genet 55:49–55
Wenzel G, Debnath SC, Schuchmann R, Foroughi-Wehr FR (1987) Combined application of classical and unconventional techniques in breeding of disease resistant potatoes. In: Richardson DE, Jellis GJ (eds) The production of new potato varieties: Technological advances. Cambridge University Press, pp 277–288
Zimmermann U, Scheurich P (1981) High frequency fusion of plant protoplasts by electric field. Planta 151:26–32
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Zuba, M., Binding, H. (1989). Isolation and Culture of Potato Protoplasts. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_8
Download citation
DOI: https://doi.org/10.1007/978-3-642-73614-8_8
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-73616-2
Online ISBN: 978-3-642-73614-8
eBook Packages: Springer Book Archive