Somatic Hybridization in Rice x Soybean

  • M. Niizeki
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 8)


Rice, Oryza sativa L., is the world’s most important cereal food crop in terms of caloric intake. It contains about 72% glucoside and 10% protein (Standard Tables of Food Composition, Agency of Science and Technology, Japan 1986). In terms of total world grain production it is exceeded only by wheat. Rice is a warm area crop and is grown most extensively in the humid tropical and subtropical regions of the world. In temperate climates, and at high elevations, rice is grown as a summer crop. About 90% of the worlD’s rice is grown in the People’s Republic of China, India, Japan, Korea and southeastern Asia, outside of Asia, less than 10% is produced in the USA, Brazil, Africa and southern Europe (EAO 1985). Soybean, Glycine max (L.) Merr., is the worlD’s most important crop as a source of plant protein and oil. It contains about 35% protein, 19% lipid and 24% of glucoside (Standard Tables of Food Composition, Agency of Science and Technology, Japan 1986). The USA now accounts for about 48% of the world production of soybeans. China and Brazil account for another 33% of the worlD’s total production. The remaining production is scattered among various countries in Asia and South America (FAO 1985).


Chloroplast Genome Somatic Hybrid Protoplast Culture Streptomycin Resistance Rice Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Abdullah R, Cocking EC, Thompson JA (1986) Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090CrossRefGoogle Scholar
  2. Akada S, Hirai A, Uchimiya H (1983) Studies on mode of separation of chloroplast genomes in para-sexual hybrid calli. 1. Fraction I protein composition in unseparated hybrid callus. Plant iSci Lett 31:223–230CrossRefGoogle Scholar
  3. Aviv D, Bleishman S, Arzee-Gonen P, Galun E (1984) Intersectional cytoplasmic hybrids in Nicotiana: Identification of plastomes and chondriomes in N. sylvestris + N. rustka cybrids having N. sylvestris nuclear genomes. Theor Appl Genet 67:499–504CrossRefGoogle Scholar
  4. Bajaj YPS (1983) Somatic hybridization and cryopreservation studies on rice × pea and wheat × pea protoplasts. In: Potrykus I, Harms CT, Hinnen A, Hutter R, King PJ, Shillito RD (eds) Protoplasts 1983, Birkhäuser, Basel, pp 248–249Google Scholar
  5. Barwale UB, Kerns HR, Widholm JM (1986) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167:473–481CrossRefGoogle Scholar
  6. Binding H, Nehls R (1978) Somatic cell hybridization of Viciafaba + Petunia hybrida. Mol Gen Genet 164:137–143CrossRefGoogle Scholar
  7. Chien YC, Kao KN, Wetter LR (1982) Chromosomal and isozyme studies of Nicotiana tabacum-Glycine max hybrid cell lines. Theor Appl Genet 62:301–304Google Scholar
  8. Chowhury VK, Widholm JM (1985) Callus production from photoautotrophic soybean cell culture protoplasts. Plant Cell Rep 4:289–292CrossRefGoogle Scholar
  9. Chu ZC, Wang CC, Sun CS, Xu Z, Zhu ZY, Yin GC, Bi FY (1975) Attempt at establishing a better medium for anther culture of rice by comparison between different nitrogen-sources. Sci Sin 2:484–490Google Scholar
  10. Constabel F, Weber G, Kirkpatrick JW, Rahl K (1976) Cell division of intergeneric protoplast fusion products. Z Pflanzenphysiol 79:1–7Google Scholar
  11. Coulibaly MY, Demarly Y (1986) Regeneration of plantlets from protoplasts of rice, Oryza sativa L. Z Pflanzenzlicht 96:79–81Google Scholar
  12. Deka PC, Sen SK (1976) Differentiation in calli originated from isolated protoplasts of rice (Oryza sativa L.) through plating technique. Mol Gen Genet 145:239–243CrossRefGoogle Scholar
  13. Donn G (1978) Cell division and callus regeneration from leaf protoplasts of Vicia narbenensis. Z Pflanzenphysiol 86:65–76Google Scholar
  14. Dudits D, Kao KN, Constabel F, Gamborg OL (1976) Fusion of carrot and barley protoplasts and division of heterokaryocytes. Can J Genet Cytol 18:263–269Google Scholar
  15. Evans DA, Sharp WR, Paddock EF (1977) Variation in callus proliferation and root morphogenesis in leaf tissue cultures of Glycine max strain T219. Phytomorphology 26:379–384Google Scholar
  16. FAO (1985) Vol. 39 Production Year bookGoogle Scholar
  17. Fluhr R, Aviv D, Edelman M, Galun E (1983) Cybrids containing mixed and sorted-out chloroplasts following interspecific somatic fusions in Nicotiana. Theor Appl Genet 65:289–294CrossRefGoogle Scholar
  18. Frearson EM, Power JB, Cocking EC (1973) The isolation, culture and regeneration of Petunia leaf protoplasts. Dev Biol 33:130–137PubMedCrossRefGoogle Scholar
  19. Fujimura T, Sakurai M, Akagi H, Negishi T, Hirose A (1985) Regeneration of rice plants from protoplasts. Plant Tissue Cult Lett 2:74–75CrossRefGoogle Scholar
  20. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158PubMedCrossRefGoogle Scholar
  21. Gamborg OL, Davis BP, Stahlhut RW (1983a) Somatic embryogenesis in cell cultures of Glycine species. Plant Cell Rep 2:209–212CrossRefGoogle Scholar
  22. Gamborg OL, Davis BP, Stahlhut RW (1983b) Cell division and differentiation in protoplasts from cell culture of Glycine species and leaf tissue of soybean. Plant Cell Rep 2:213–215CrossRefGoogle Scholar
  23. Hirai A (1982) Isoelectrofocusing of non-carboxymethylated fraction I protein from green callus. Plant Sci Lett 25:37–41CrossRefGoogle Scholar
  24. Horn ME, Sherrard JH, Widholm JM (1983) Photoautotrophic growth of soybean cells in suspension culture. Plant Physiol 72:426–429PubMedCrossRefGoogle Scholar
  25. Imamura J (1984) Callus formation from tobacco (nia 115) + Triticum durum fusion hybrid. Plant Tissue Cult Lett 1:60–61CrossRefGoogle Scholar
  26. Kao KN (1977) Chromosomal behaviour in somatic hybrids of soybean-Nicotiana glauca. Mol Gen Genet 150:225–230CrossRefGoogle Scholar
  27. Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115:355–367CrossRefGoogle Scholar
  28. Kao KN, Michayluk M (1975) Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta 126:105–110CrossRefGoogle Scholar
  29. Kao KN, Keller WA, Miller RA (1970) Cell division in newly formed cells from protoplasts of soybean. Exp Cell Res 62:338–340PubMedCrossRefGoogle Scholar
  30. Kao KN, Constabel F, Michayluk MR, Gamborg OL (1974) Plant protoplast fusion and growth of intergeneric hybrid cells. Planta 120:215–227CrossRefGoogle Scholar
  31. Kartha KK, Gamborg OL, Constabel F, Kao KN (1974) Fusion of rape seed and soybean protoplasts and subsequent division of heterokaryocytes. Can J Bot 52:2435–2436CrossRefGoogle Scholar
  32. Kyozuka J, Hayashi Y, Shimamoto K (1987) High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Theor Appl Genet 206:408–413Google Scholar
  33. Linsmaier EF, Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol Plant 18:100–127CrossRefGoogle Scholar
  34. Lu DY, Cooper-Bland S, Pental D, Cocking EC, Davey RM (1983) Isolation and sustained division of protoplasts from cotyledons of seedlings and immature seeds of Glycine max L. Z Pflanzenphysiol 111:389–394Google Scholar
  35. Maliga P, Lörz H, Lazak G, Nagy F (1982) Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana. Mol Gen Genet 185:211–215CrossRefGoogle Scholar
  36. Medgyesy P, Menczel L, Maliga P (1980) The use of cytoplasmic streptomycin resistance. Chloroplast transfer from N. tabacum into N. sylvestris and isolation of their somatic hybrids. Mol Gen Genet 179:693–698CrossRefGoogle Scholar
  37. Menczel L, Nagy F, Kiss ZR, Maliga P (1981) Streptomycin resistant and sensitive somatic hybrids of AT. tabacum + N. knightiana. Correlation of resistance to N. tabacum plastids. Theor Appl Genet 59:191–195CrossRefGoogle Scholar
  38. Morel G, Wetmore RH (1951) Fern tissue culture. Am J Bot 38:141–143CrossRefGoogle Scholar
  39. Muller AJ, Grafe R (1978) Isolation and characterization of cell lines of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161:67–76CrossRefGoogle Scholar
  40. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar
  41. Niizeki M, Kita F (1981) Cell division of rice and soybean and their fused protoplasts. Jpn J Breed 31:161–167Google Scholar
  42. Niizeki M, Kita F, Takahashi M (1982) Cell division in fused protoplasts of rice and soybean and their selection system. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 629–630Google Scholar
  43. Niizeki M, Tanaka M, Akada S, Hirai A, Saito K (1985) Callus formation of somatic hybrid of rice and soybean and characteristics of the hybrid callus. Jpn J Genet 60:81–92CrossRefGoogle Scholar
  44. Niizeki M, Tanaka M, Saito K (1986) Response of somatic hybrid callus between rice and soybean to streptomycin. Jpn J Breed 36:75–79Google Scholar
  45. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163:85–87PubMedCrossRefGoogle Scholar
  46. Ohira K, Ojima K, Fujiwara A (1973) Studies on the nutrition of rice cell culture I. A simple, defined medium for rapid growth in suspension culture. Plant Cell Physiol 14:1113–1121Google Scholar
  47. Schenk RU, Hildebrandt AC (1972) Medium and technique for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can J Bot 50:199–204CrossRefGoogle Scholar
  48. Shillito RD, Paszkowski J, Potrykus I (1983) Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species. Plant Cell Rep 2:244–247CrossRefGoogle Scholar
  49. Toriyama K, Hinata K (1985) Cell suspension and protoplast culture in rice. Plant Sci 41:179–183CrossRefGoogle Scholar
  50. Tsai CK, Chien YC, Chou YL, Wu SX (1978) A further study on the isolation and culture of rice (Oryza sativa L.) protoplast. In: Proc Symp Plant Tissue Cult. Science Press, Peking, pp 317–324Google Scholar
  51. Wakasa K, Kobayashi K, Kanda H (1984) Colony formation from protoplasts of nitrate reductase deficient rice cell lines, J Plant Physiol 117:223–231Google Scholar
  52. Wetter LR (1977) Science Press, PekingIsozyme patterns in soybean-Nicotiana somatic hybrid cell lines. Mol Gen Genet 150:231–235CrossRefGoogle Scholar
  53. Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5:150–154CrossRefGoogle Scholar
  54. Xu ZH, Davey MR, Cocking EC (1982) Callus formation from root protoplasts of Glycine max (soybean). Plant Sci Lett 24:111–115CrossRefGoogle Scholar
  55. Yamada Y, Yang ZQ Tang DT (1985) Regeneration of rice plants from protoplasts. Rice Genet Newslett 2:94Google Scholar
  56. Yamada Y, Yang ZQ, Tang DT (1986) Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep 5:85–88CrossRefGoogle Scholar
  57. Zieg RG, Outka DE (1980) The isolation, culture and callus formation of soybean pod protoplasts. Plant Sci Lett 18:105–114CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • M. Niizeki
    • 1
  1. 1.Plant Breeding Laboratory, Faculty of AgricultureHirosaki UniversityHirosaki Aomori-ken 036Japan

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