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Ultrastructural Studies of Plant Protoplast Fusion

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Book cover Plant Protoplasts and Genetic Engineering I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 8))

Abstract

A wide variety of plant protoplasts have been fused and in many cases hybrid plants have been regenerated (see reviews by Fowke and Gamborg 1980; Sehieder and Vasil 1980; Gleba and Sytnik 1984; Constabel and Cutler 1985; Bajaj et al. 1988). A number of different fusion techniques have been reported; however, the most effective involves the use of polyethylene glycol (PEG) (Kao and Michayluk 1974; Wallin et al. 1974). PEG-induced fusion is non-specific and has been used to fuse protoplasts of widely separated plant species and genera, animal cells and combinations of plant protoplasts and animal cells. Recently, electrofusion has also been used successfully to fuse protoplasts, and regeneration of hybrids has been achieved (e.g. Zimmerman et al. 1984; Schieder and Kohn 1986; De Vries and Tempelaar 1987). The major emphasis of the fusion work to date has been the development of techniques to produce hybrid plants which can be used in plant breeding programmes.

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Authors and Affiliations

  1. Department of Biology, University of Saskatchewan, Saskatoon, Canada, S7N OWO

    L. C. Fowke

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  1. L. C. Fowke
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  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1989 Springer-Verlag Berlin Heidelberg

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Fowke, L.C. (1989). Ultrastructural Studies of Plant Protoplast Fusion. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_20

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  • DOI: https://doi.org/10.1007/978-3-642-73614-8_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73616-2

  • Online ISBN: 978-3-642-73614-8

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