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Enzymes for the Isolation of Protoplasts

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Plant Protoplasts and Genetic Engineering I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 8))

Abstract

Cocking (1960) first used enzymes to isolate higher plant protoplasts. He applied culture filtrates of a cellulolytic fungus Myrothecium verrucaria to release protoplasts from tomato root tips. Later, Takebe and co-workers succeeded in applying commercially available cell wall-degrading enzymes, Macerozyme and Cellulase Onozuka, to isolate large quantities of protoplasts from a wide variety of plant tissues (Takebe et al. 1968; Otsuki and Takebe 1969). Thereafter, many enzmye preparations have been produced commercially and used successfully for the isolation of plant protoplasts. These enzmyes are divided into three categories: cellulases, hemicellulases and pectinases by their catalytic actions (Table 1). Combined use of these enzymes and development of isolation procedures have made

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Authors and Affiliations

  1. Research & Development Division, Kikkoman Corporation, 399 Noda, Noda-shi, Chiba-ken, 278, Japan

    S. Ishii

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  1. S. Ishii
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  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1989 Springer-Verlag Berlin Heidelberg

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Ishii, S. (1989). Enzymes for the Isolation of Protoplasts. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_2

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  • DOI: https://doi.org/10.1007/978-3-642-73614-8_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73616-2

  • Online ISBN: 978-3-642-73614-8

  • eBook Packages: Springer Book Archive

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