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Isolation and Culture of Protoplasts of Forest Tree Species

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Plant Protoplasts and Genetic Engineering I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 8))

Abstract

The development of techniques in molecular biology and biotechnology and their application to plant genetic improvement promise to be of singular importance in the future of forest genetics (Karnosky 1981; Kirby 1982; Ahuja 1984a; Libby and Rauter 1984; Powledge 1984; Torrey 1985; Bajaj 1986; Kirby and David 1987). Mass clonal propagation, in vitro selection, somatic habridization, somaclonal and gametoclonal variation and genetic transformation using suitable vectors are only a few of the procedures having direct applications in crop improvement today. A key element in applying these techniques to forest tree improvement is establishment of successful procedures for isolation of protoplasts of important forest species and for their culture and the regeneration of whole plants from protoplasts. This chapter will (1) summarize reports of successful protoplast isolation and culture for forest species by focussing on recent advances, and (2) discuss long-range applications of protoplast techniques to tree improvement.

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Author information

Authors and Affiliations

  1. Department of Biological Sciences, Rutgers University, Newark, New Jersey, USA

    Edward G. Kirby

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  1. Edward G. Kirby
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  2. Michael A. Campbell
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  3. Ricardo M. Penchel
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Editor information

Editors and Affiliations

  1. A-137 New Friends Colony, New Delhi, 110065, India

    Y. P. S. Bajaj

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© 1989 Springer-Verlag Berlin Heidelberg

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Kirby, E.G., Campbell, M.A., Penchel, R.M. (1989). Isolation and Culture of Protoplasts of Forest Tree Species. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering I. Biotechnology in Agriculture and Forestry, vol 8. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73614-8_18

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  • DOI: https://doi.org/10.1007/978-3-642-73614-8_18

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-73616-2

  • Online ISBN: 978-3-642-73614-8

  • eBook Packages: Springer Book Archive

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