Recent Advances in the Isolation and Culture of Protoplasts and Their Implications in Crop Improvement

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 8)


Since the first report on the regeneration of complete plants from isolated protoplasts (Takebe et al. 1971), tremendous progress has been made and a number of crops of commercial importance such as potato, tomato, tobacco, rice, linseed, alfalfa, cucumber, eggplant, lettuce and Brassica species etc. have been routinely regenerated. This has paved the way for their genetic manipulation. Consequently considerable interest has generated in two areas, i.e., the culture of protoplasts of cereals and those of trees, and a large volume of information has accumulated recently (Table 1). This has been made possible for a number of reasons, primarily due to the upsurge of activity in plant biotechnology (see Bajaj 1986a), commercial interest, the tremendous increase in the number of workers during the last 5 years, advancement of in vitro technology and automation. Moreover, it has created awareness in agriculture, especially of plant breeders. There has been inflow of funds and finances, both from the private and the government agencies which has resulted in setting up of centres of biotechnology in various countries. This has contributed a great deal for the promotion of in vitro technology which has, in fact, now become a new trend in plant science research.


Somatic Hybrid Somaclonal Variation Mesophyll Protoplast Protoplast Culture Plant Cell Tissue Organ Cult 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
    • 2
  1. 1.Punjab Agricultural UniversityLudhianaIndia
  2. 2.New DelhiIndia

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