Antiviral Activity of Human Fibroblast Interferon in Coxsackievirus B3-Infected Cultured Human Heart Cells

  • P. Kirschner
  • R. Kandolf
  • A. Canu
  • A. Heim
  • P. H. Hofschneider


Effective antiviral chemotherapy has not yet been established in the treatment of viral heart disease. The optimal goal of antiviral treatment — to restore function to the infected cells — usually appears unattainable. A realistic goal would be to inhibit virus replication and thus prevent virus spread to as yet uninfected cells. This is achieved, in part, in many natural virus infections by the endogenous interferon (IFN) system [1, 2].


Antiviral Activity Herpes Zoster Beat Frequency Specific Host Cell Infectious Progeny Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Joklik WK (1986) Interferons. In: Fields BN, Knipe DM (eds) Fundamental Virology. Raven Press, New York, pp 281–307Google Scholar
  2. 2.
    Gresser I (1984) Role of interferon in resistance to viral infection in vivo. In: Vilcek J, De Maeyer E (eds) Interferon Vol 2, Interferons and the Immune System. Elsevier, Amsterdam, pp 221–247Google Scholar
  3. 3.
    Kandolf R, Canu A, Hofschneider PH (1985) Coxsackie B3 virus can replicate in cultured human foetal heart cells and is inhibited by interferon. J Mol Cell Cardiol 17: 167–181PubMedCrossRefGoogle Scholar
  4. 4.
    Wynne J, Braunwald E (1980) The cardiomyopathies and myocarditides. In: Braunwald E (ed) Heart Disease. A Textbook of Cardiovascular Medicine. W. B. Saunders, Philadelphia, pp 1437–1498Google Scholar
  5. 5.
    Kandolf R, Hofschneider PH (1984) Effect of interferon on the replication of Coxsackie B3 virus in cultured human fetal heart cells. In: Bolte HD (ed) Viral Heart Disease. Springer, Berlin, pp 57–63Google Scholar
  6. 6.
    Kasten FH (1973) Mammalian myocardial cells. In: Kruse PFJ, Patterson MKJ (eds) Tissue Culture, Methods and Applications. Academic Press, New York, pp 72–81Google Scholar
  7. 7.
    Kandolf R, Hofschneider PH (1985) Molecular cloning of the genome of a cardiotropic Coxsackie B3 virus: Full-length reverse-transcribed recombinant cDNA generates infectious virus in mammalian cells. Proc Natl Acad Sci USA 82: 4818–4822PubMedCrossRefGoogle Scholar
  8. 8.
    Takemoto KK, Habel K (1959) Virus-cell relationship in a carrier culture of HeLa cells and Coxsackie A9 virus. Virology 7: 28–44PubMedCrossRefGoogle Scholar
  9. 9.
    Goldberg RJ, Crowell RL (1971) Susceptibility of differentiating muscle cells of fetal mouse in culture to coxsackievirus A13. J Virol 7: 759–769PubMedGoogle Scholar
  10. 10.
    Kandolf R, Ameis D, Kirschner P, Canu A, Hofschneider PH (1987) In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: An approach to the diagnosis of viral heart disease. Proc Natl Acad Sci USA 84: 6272–6276PubMedCrossRefGoogle Scholar
  11. 11.
    Blalock JE, Stanton JD (1980) Common pathways of interferon and hormonal action. Nature 283: 406–408PubMedCrossRefGoogle Scholar
  12. 12.
    Lampidis TJ, Brouty-Boyé D (1981) Interferon inhibits cardiac cell function in vitro. Proc Soc Exp Biol Med 166: 181–185PubMedGoogle Scholar
  13. 13.
    Kandolf R, Kirschner P, Ameis D, Canu A, Hofschneider PH (1987) Cultured human heart cells: a model system for the study of the antiviral activity of interferons. Eur Heart J 8, Supp J: 453–456Google Scholar
  14. 14.
    Treuner J, Dannecker G, Joester KE, Hettinger A, Niethammer D (1981) Pharmacological aspects of clinical stage I/II trials with human beta interferon in children. J Interferon Res 1: 373–380PubMedCrossRefGoogle Scholar
  15. 15.
    Heidemann E, Wilms K, Treuner J, Niethammer D (1982) Fibroblasten-Interferon zur Behandlung des Herpes Zoster. Dtsche Med Wochenschr 107: 695–697CrossRefGoogle Scholar
  16. 16.
    Heidemann E, Obert HJ (1985) Clinical trials and pilot studies with natural interferons in Germany. In: Kirchner H, Schellekens H (eds) The Biology of the Interferon System 1984. Elsevier, Amsterdam, pp 557–567Google Scholar
  17. 17.
    Aguet M, Groebke M, Dreiding P (1983) Down-regulation of interferon receptors. In: De Maeyer E, Schellekens H (eds) The Biology of the Interferon System 1983. Elsevier, Amsterdam, pp 161–170Google Scholar
  18. 18.
    Kahn CR (1979) The role of insulin receptors and receptor antibodies in states of altered insulin action. Proc Soc Exp Biol Med 162: 13–21PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • P. Kirschner
    • 1
  • R. Kandolf
    • 1
    • 2
  • A. Canu
    • 3
  • A. Heim
    • 1
    • 2
  • P. H. Hofschneider
    • 1
  1. 1.Department of Virus ResearchMax-Planck-Institut für BiochemieMartinsriedGermany
  2. 2.Department of Internal Medicine IUniversity of MunichMünchen 70Germany
  3. 3.Département de MicrobiologieUnité de Formation et de Recherche des Sciences PharmaceutiquesCaenFrance

Personalised recommendations