Characterization of T-Lymphocytes Cultured from Human Endomyocardial Biopsies

  • J. T. Fallon
  • I. Stamenkovic
  • D. M. Frisman
  • C. Leary
  • I. Palacios
  • J. T. Kurnick


Myocarditis is often characterized by a lymphocytic inflammatory infiltrate in myocardial tissue. However, human myocarditis has neither a definitive etiologic agent nor a well-characterized pathogenesis [1]. Previous investigations of clinical myocarditis have centered around potential viral etiologies and the humoral immune aspects of the acute disease and its possible sequelae, namely dilated cardiomyopathy [2, 3, 4]. A few investigations have examined circulating T lymphocytes in patients with dilated cardiomyopathy [5, 6] while others have applied immunohistochemical techniques to characterize the inflammatory infiltrates in endomyocardial biopsies of myocarditis [7, 8, 9].


Dilate Cardiomyopathy Endomyocardial Biopsy Viral Myocarditis Lymphocyte Clone Lauryl Sarcosinate 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Abelmann WH (1973) Viral myocarditis and its sequelae. Annu Rev Med 24: 145–150PubMedCrossRefGoogle Scholar
  2. 2.
    Woodruff J (1980) Viral myocarditis. A review. Am J Pathol 101: 427–479Google Scholar
  3. 3.
    Cambridge G, MacArthur CGC, Waterson AP et al. (1979) Antibodies to Coxsackie B viruses in congestive cardiomyopathy. Br Heart J 41: 692–696PubMedCrossRefGoogle Scholar
  4. 4.
    Maisch B, Deeg P, Liebau G, Kochsiek K (1983) Diagnostic relevance of humoral and cytotoxic immune reactions in primary and secondary dilated cardiomyopathy. Am J Cardiol 52: 1072–1078PubMedCrossRefGoogle Scholar
  5. 5.
    Eckstein R, Mempel W, Bolte HD (1982) Reduced suppressor cell activity in congestive cardiomyopathy and myocarditis. Circulation 65: 1224–1229PubMedCrossRefGoogle Scholar
  6. 6.
    Anderson JL, Carlquist JF, Higashikubo R (1985) Quantitation of lymphocyte subsets by immunofluorescence flow cytometry in idiopathic dilated cardiomyopathy. Am J Cardiol 55: 1550–1554PubMedCrossRefGoogle Scholar
  7. 7.
    Marboe CC, Knowles DM, Weiss MB et al. (1984) Characterization of the inflammatory infiltrate in human myocarditis—an endomyocardial biopsy study. In: Viral heart disease. Springer, Berlin Heidelberg New York, pp 74–82Google Scholar
  8. 8.
    Cassling RS, Linder J, Sears TD et al. (1985) Quantitative evaluation of inflammation from idiopathically failing or irritable hearts: experience in 80 pediatric and adult patients. Am Heart J 110: 713–720PubMedCrossRefGoogle Scholar
  9. 9.
    Schnitt SJ, Ciano PD, Schoen FJ (1987) Quantitation of lymphocytes in endomyocardial biopsies: use and limitations of antibodies to leucocyte common antigen. Hum Pathol 18: 796–800PubMedCrossRefGoogle Scholar
  10. 10.
    Kurnick JT, Leary C, Palacios IF, Fallon JT (1987) Culture and characterization of lymphocytic infiltrates from endomyocardial biopsies of patients with idiopathic myocarditis. Eur Heart J 8 [Suppl J]: 135–139Google Scholar
  11. 11.
    Mayer TG, Fuller AA, Fuller TC et al. (1985) Characterization of in vivo-activated allospecific T lymphocytes propagated from human renal allograft biopsies undergoing rejection. J Immunol 134: 258–264PubMedGoogle Scholar
  12. 12.
    Kurnick JT, Grönvik KG, Kimura AK et al. (1979) Long term growth in vitro of human T cell blasts with maintanence of specificity and function. J Immunol 122: 1255–1260PubMedGoogle Scholar
  13. 13.
    Kurnick JT, Warrens AR, Moscicki RA, Leary CP (1983) T cell subsets in human lymphocytes maintained in IL-2 medium after PHA mixed lymphocyte reaction activation. Clin Immunol Immunopathol 27: 444–451PubMedCrossRefGoogle Scholar
  14. 14.
    Stamenkovic I, Stegagno M, Wright KA et al. (1988) Clonal dominance among T lymphocyte infiltrates in arthritis. Proc Natl Acad Sci USA 85: 1179–1183PubMedCrossRefGoogle Scholar
  15. 15.
    Marrack P, Kappler J (1987) The t cell receptor. Science 238: 1073–1079PubMedCrossRefGoogle Scholar
  16. 16.
    Zeevi A, Fung J, Zerbe TR et al. (1986) Allospecificity of activated T cells grown from endomyocardial biopsies from heart transplant patients. Transplantation 41: 620–626PubMedCrossRefGoogle Scholar
  17. 17.
    Kurnick JT, Kradin RL, Blumberg R et al. (1986) Functional characterization of T lymphocytes propagated from human lung carcinoma. Clin Immunol Immunopath 38: 367–380CrossRefGoogle Scholar
  18. 18.
    Herberman RB, Ortaldo JR (1981) Natural killer cells: their role in defenses against disease. Science 214: 24–30PubMedCrossRefGoogle Scholar
  19. 19.
    Levinson A, Silver D, Seed B (1984) Minimal size plasmids containing an M13 origin for production of single-strand transducing particles. J Mol Appl Genet 2: 507–517PubMedGoogle Scholar
  20. 20.
    Reed KC, Mann DA (1985) Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res 13: 7207–7221PubMedCrossRefGoogle Scholar
  21. 21.
    Caves PK, Schultz WP, Dong E Jr, Stinson EB, Shumway NE (1974) New instrument for transvenous cardiac biopsy. Am J Cardiol 33: 264–267PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • J. T. Fallon
    • 1
  • I. Stamenkovic
    • 1
  • D. M. Frisman
    • 1
  • C. Leary
    • 1
  • I. Palacios
    • 1
  • J. T. Kurnick
    • 1
  1. 1.Department of Pathology, Department of Molecular Biology, Immunopathology Unit and Cardiac UnitMassachusetts General HospitalBostonUSA

Personalised recommendations