Detection and Typing of Enteroviruses by Nucleic Acid Probes and Monoclonal Antibodies
Enteroviruses are currently detected using virus isolation followed by a neutralization test with a panel of antisera for specific typing of the virus strain. One of the problems in this method is the long time period needed for final diagnosis. In addition some coxsackievirus (CV) type A (CVA) strains can only be isolated in newborn mice. Gene technology and hybridoma methods offer new possibilities for development of reagents which can be used in enterovirus detection. The increasing knowledge on the genetic and antigenic structure, and mutual relatedness of enteroviruses further facilitates the applicability of these techniques.
KeywordsSilver Enhancement Nucleic Acid Probe Spot Hybridization Enterovirus Serotypes Echo Virus
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