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Detection and Typing of Enteroviruses by Nucleic Acid Probes and Monoclonal Antibodies

  • T. Hyypiä
  • M. Kallajoki
  • P. Auvinen
Conference paper

Abstract

Enteroviruses are currently detected using virus isolation followed by a neutralization test with a panel of antisera for specific typing of the virus strain. One of the problems in this method is the long time period needed for final diagnosis. In addition some coxsackievirus (CV) type A (CVA) strains can only be isolated in newborn mice. Gene technology and hybridoma methods offer new possibilities for development of reagents which can be used in enterovirus detection. The increasing knowledge on the genetic and antigenic structure, and mutual relatedness of enteroviruses further facilitates the applicability of these techniques.

Keywords

Silver Enhancement Nucleic Acid Probe Spot Hybridization Enterovirus Serotypes Echo Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • T. Hyypiä
    • 1
  • M. Kallajoki
    • 2
  • P. Auvinen
    • 1
  1. 1.Departments of VirologyUniversity of TurkuTurkuFinland
  2. 2.Departments of PathologyUniversity of TurkuTurkuFinland

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