Abstract
Enteroviruses are currently detected using virus isolation followed by a neutralization test with a panel of antisera for specific typing of the virus strain. One of the problems in this method is the long time period needed for final diagnosis. In addition some coxsackievirus (CV) type A (CVA) strains can only be isolated in newborn mice. Gene technology and hybridoma methods offer new possibilities for development of reagents which can be used in enterovirus detection. The increasing knowledge on the genetic and antigenic structure, and mutual relatedness of enteroviruses further facilitates the applicability of these techniques.
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References
Bowles NE, Richardson PJ, Olsen EGJ, Archard LC (1986) Detection of Coxsackie-B-virus-specific RNA sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. Lancet I: 1120–1123
Cova L, Kopecka H, Aymard M, Girard M (1988) Use of cRNA probes for the detection of enteroviruses by molecular hybridization. J Med Virol 24: 11–18
Easton AJ, Eglin RP (1988) The detection of coxsackievirus RNA in cardiac tissue by in situ hybridization. J Gen Virol 69: 285–291
Hyypiä T, Stålhandske P, Vainionpää R, Pettersson U (1984) Detection of enteroviruses by spot hybridization. J Clin Microbiol 19: 436–438
Hyypiä T, Maaronen M, Auvinen P, Stålhandske P, Pettersson U, Stanway G, Hughes P, Ryan M, Almond J, Stenvik M, Hovi T (1987) Nucleic acid sequence relationships between enterovirus serotypes. Mol Cell Probes 1: 169–176
Kandolf R, Ameis D, Kirschner P, Canu A, Hofschneider PH (1987) In situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease. Proc Natl Acad Sci USA 84: 6272–6276
Khatib R, Probert A, Reyes MP, Khatib G, Chason JL (1987) Mouse strain variation as a factor in the pathogenesis of coxsackievirus B3 murine myocarditis. J Gen Virol 68: 2981–2988
Kohne DE, Gibbs CJ, White L, Tracy SM, Meinke W, Smith RA (1981) Virus detection by nucleic acid hybridization: examination of normal and ALS tissues for the presence of poliovirus. J Gen Virol 56: 223–233
Maples JA (1985) A method for the covalent attachment of cells to glass slides for use in immunohistochemical assays. Am J Clin Pathol 83: 356–363
Prabhakar BS, Haspel MV, McClintock PR, Notkins AL (1982) High frequency of antigenic variants among naturally occurring human coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374–376
Rotbart HA, Levin MJ, Villarreal LP (1984) Use of subgenomic poliovirus DNA hybridization probes to detect the major subgroups of enteroviruses. J Clin Microbiol 20: 1105–1108
Saiki RK, Scharf S, Faloona F, Mullis KB, Horn GT, Erlich HA, Arnheim N (1985) Enzymatic amplification of, β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science 230: 1350–1354
Sandberg M, Vuorio E (1987) Localization of types I, II, and III collagen mRNAs in developing human skeletal tissues by in situ hybridization. J Cell Biol 104: 1077–1084
Ståhandske POK, Lindberg M, Pettersson U (1984) Replicase gene of coxsackievirus B3. J Virol 51: 742–746
Strebel K, Beck E, Strohmaier K, Schaller H (1986) Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins. J Virol 57: 983–991
Tracy S (1984) A comparison of genomic homologies among the coxsackievirus B group: use of fragments of the cloned coxsackievirus B3 genome as probes. J Gen Virol 65: 2167–2172
Ukkonen P, Huovilainen A, Hovi T (1986) Detection of poliovirus antigen by enzyme immunoassay. J Clin Microbiol 24: 954–958
Yousef GE, Brown IN, Mowbray JF (1987) Derivation and biochemical characterisation of an enterovirus group-specific monoclonal antibody. Intervirology 28: 163–170
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© 1988 Springer-Verlag Berlin Heidelberg
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Hyypiä, T., Kallajoki, M., Auvinen, P. (1988). Detection and Typing of Enteroviruses by Nucleic Acid Probes and Monoclonal Antibodies. In: Schultheiß, HP. (eds) New Concepts in Viral Heart Disease. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-73610-0_11
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DOI: https://doi.org/10.1007/978-3-642-73610-0_11
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