Electron Microscopy in Childhood Tumors

  • M. Us-Krašovec
Conference paper


The management of childhood malignancies continues to improve. There is increased survival for many tumors and in some cases patients may even be cured. However, the therapeutic regimes may have significant adverse effects resulting in a variety of psychological and physical sequelae. Accurate morphologic diagnosis is therefore mandatory before embarking on a definitive treatment course. A pa thologist today has not only to solve the basic dilemma of whether the tumor is be nign or malignant but also to assess its histogenesis and differentiation. To achieve this, he often has to employ all the available modern diagnostic techniques: electron microscopy, immunohistochemistry, cytogenetics, etc. [18, 34].


Aspiration Biopsy Fine Needle Aspirate Round Cell Tumor Childhood Tumor Physical Sequela 
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  1. 1.
    Akhtar M, Ashraf MA, Owen E, Bakry M (1980) A simple method for processing fine-needle aspiration biopsy specimens for electron microscopy. J Clin Pathol 33:1214–1216PubMedCrossRefGoogle Scholar
  2. 2.
    Akhtar M, Ashraf A, Owen EW (1981) Application of electron microscopy in the interpretation of fine-needle aspiration biopsies. Cancer 48: 2458–2463PubMedCrossRefGoogle Scholar
  3. 3.
    Akhtar M, Ali M, Sabbah R (1985) Aspiration cytology of Ewing’s sarcoma. Light and electron microscopic correlations. Cancer 56: 2051–2060PubMedCrossRefGoogle Scholar
  4. 4.
    Akhtar M, Ali M, Sabbah R et al. (1985) Fine-needle aspiration biopsy (FNAB) diagnosis of round cell malignant tumors of childhood: a combined light and electron microscopic approach. Cancer 55: 1805–1817PubMedCrossRefGoogle Scholar
  5. 5.
    Akhtar M, Ali M, Sabbah RS et al. (1986) Aspiration cytology of neuroblastoma. Light and electron microscopic correlations. Cancer 57: 797–803PubMedCrossRefGoogle Scholar
  6. 6.
    Akhtar M, Bakry M, Nash EJ (1986) An improved technic for processing aspiration biopsy for electron microscopy. Am J Clin Pathol 85: 57–60PubMedGoogle Scholar
  7. 7.
    Altmannsberger M, Osborn M, Treuner J, Hölscher A, Weber K, Schauer A (1982) Diagnosis of human childhood rhabdomyosarcoma by antibodies to demin. The structural protein of muscle specific intermediate filaments. Virchows Arch [B] 39: 203–215CrossRefGoogle Scholar
  8. 8.
    Altmannsberger M, Osborn M, Droese M, Weber K, Schauer A (1984) Diagnostic value of intermediate filaments antibodies in clinical cytology. Klin Wochenschr 62: 114–123PubMedCrossRefGoogle Scholar
  9. 9.
    Baumal R, Khan HJ, Bailey D et al. (1984) The value of immunohistochemistry in increasing diagnostic precision of indifferentiated tumors by surgical pathologists. Histochem J 16: 1061–1078PubMedCrossRefGoogle Scholar
  10. 10.
    Berkman WA, Chowdhury L, Brow NL, Padleckas R (1983) Value of electron microscopy in cytologic diagnosis of fine-needle biopsy. Am J Roentgenol 140: 1253–1258Google Scholar
  11. 11.
    Bundtzen JL, Norback DH (1982) The ultrastructure of poorly differentiated rhabdomyosarcoma: a case report and literature review. Hum Pathol 13: 301–313PubMedCrossRefGoogle Scholar
  12. 12.
    Chess Q, Hajdu SI (1986) The role of immunoperoxidase staining in diagnostic cytology. Acta Cytol (Baltimore) 30:1–7Google Scholar
  13. 13.
    Collins VP, Ivarsson B (1981) Tumor classification by electron microscopy of fine needle aspiration biopsy material. Acta Pathol Microbiol Scand [A] 89: 103–105Google Scholar
  14. 14.
    Dalquen P (1987) Immunocytochemical cytodiagnosis of tumors. In: Seifert G, Hübner K (eds) Pathology of cell receptors and tumor markers. Fisher, Stuttgart, pp 196–201Google Scholar
  15. 15.
    De Jong ASH, Kessel-van Vark M, Albus-Lutter CE (1985) Skeletal muscle actin as tumor marker in the diagnosis of rhabdomyosarcoma in childhood. Am J Surg Pathol 9: 467–474PubMedCrossRefGoogle Scholar
  16. 16.
    Denk H, Krepler R, Artlieb U et al. (1983) Proteins of intermediate filaments. Am J Pathol 110: 193–208PubMedGoogle Scholar
  17. 17.
    Domagala W, Lubinski J, Weber K, Osborn M (1986) Intermediate filament typing of tumor cells in fine needle aspirates by means of monoclonal antibodies. Acta Cytol (Baltimore) 30: 214–224Google Scholar
  18. 18.
    Favara BE (1976) Diagnostic methods in pediatric oncology. Pediatr Clin North Am 23: 55–65PubMedGoogle Scholar
  19. 19.
    Hagelquist E (1978) Light and electron microscopy studies on material obtained by fine needle biopsy. Acta Otolaryngol [Suppl] (Stockh) 354: 5–75Google Scholar
  20. 20.
    Jereb B, Us-Krašovec M, Jereb M (1978) Thin needle biopsy of solid tumors in children. Med Pediatr Oncol 4: 213–220PubMedCrossRefGoogle Scholar
  21. 21.
    Kahn HJ, Yeger H, Kassim D et al. (1983) Immunohistochemical and electron microscopic assessment of childhood rhabdomyosarcoma. Cancer 51: 1897–1903PubMedCrossRefGoogle Scholar
  22. 22.
    Kindblom LG (1983) Light and electron microscopic examination of embeded fine-needle aspiration biopsy specimens in the preoperative diagnosis of soft tissue and bone tumors. Cancer 51: 2264–2277PubMedCrossRefGoogle Scholar
  23. 23.
    Lazzaro AU (1983) Improved preparation of fine needle aspiration biopsies for transmission electron microscopy. Pathology 15: 399–402PubMedCrossRefGoogle Scholar
  24. 24.
    Leong AS (1986) Antibody probes in the diagnosis of anaplastic tumors. I. malignant round cell tumors. Pathology 18: 296–305PubMedCrossRefGoogle Scholar
  25. 25.
    Llombart-Bosch A, Blache R, Peydro-oaya A (1978) Ultrastructural study of 28 cases of Ewing’s sarcoma: typical and atypical forms. Cancer 41: 1362–1373PubMedCrossRefGoogle Scholar
  26. 26.
    Mierau GW, Favara BE (1980) Rhabdomyosarcoma in children. Ultrastructural study of 31 cases. Cancer 46: 2035–2040PubMedCrossRefGoogle Scholar
  27. 27.
    Misugi I, Misugi N, Newton WA (1968) Fine structure of neuroblastoma, ganglioneuroblastoma and pheochromocytoma. Arch Pathol 86: 160–169PubMedGoogle Scholar
  28. 28.
    Morales AR, Fine G, Horn RC (1972) Rhabdomyosarcoma: an ultrastructural appraisal. Pathol Annu 7: 81–106PubMedGoogle Scholar
  29. 29.
    Nordgren H, Akerman M (1982) Electron microscopy of fine needle aspiration biopsy from soft tissue tumors. Acta Cytol (Baltimore) 26: 179–188Google Scholar
  30. 30.
    Picker JL, Weiss LM, Medeiros LJ et al. (1987) Immunophenotypic criteria for the diagnosis of non-Hodgkin’s lymphoma. Am J Pathol 128: 181–201PubMedGoogle Scholar
  31. 31.
    Romansky SG, Crocker DW, Shaw KNF (1978) Ultrastructural studies of neuroblastoma. Cancer 42: 2392–2398PubMedCrossRefGoogle Scholar
  32. 32.
    Sehested M, Francis P, Hainau B (1983) Electron microscopy of trans-thoracic fine needle aspiration biopsies. Acta Pathol Microbiol Scand [A] 91: 457–461Google Scholar
  33. 33.
    Taylor SR, Nunez C (1984) Fine-needle aspiration biopsy in a pediatric population. Cancer 54: 1449–1453PubMedCrossRefGoogle Scholar
  34. 34.
    Triche TJ, Askin FB, Kissane JM (1986) Neuroblastoma. Ewing’s sarcoma, and the differential diagnosis of small-round-blue cell tumors. In: Finegold MS (ed) Major problems in pathology: pathology of neoplasia in children and adolescents. Saunders, Philadelphia, pp 145–195Google Scholar
  35. 35.
    Tsokos M, Linnoila RI, Chandra RS, Triche JT (1984) Neuronspecifíc enolase in the diagnosis of neuroblastoma and other small, round-cell tumors in children. Hum Pathol 15: 575–584PubMedCrossRefGoogle Scholar
  36. 36.
    Us-Krašovec M, Golouh R (1983) Cytology of small round-cell tumors of childhood (Abstract). Pathol Res Pract 178: 169Google Scholar
  37. 37.
    Valkov I, Bojikin B (1987) Fine-needle aspiration biopsy of abdominal and retroperitoneal tumors in infants and children. Diagn Cytopathol 3: 129–133PubMedCrossRefGoogle Scholar
  38. 38.
    Wills EJ, Carr S, Philips J (1987) Electron microscopy in the diagnosis of percutaneous fine needle aspiration specimens. Ultrastruct Pathol 11: 361–387PubMedCrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1988

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  • M. Us-Krašovec

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